Taxonomic and phylogenic appraisal of a novel species and a new record of Stictidaceae from coffee in Yunnan Province, China Author Lu, Li 0000-0003-0977-6414 Innovative Institute of Plant Health, Zhongkai University, Guangdong, P. R. China & Centre for Mountain Futures, Kunming Institute of Botany, Kunming 650201, Yunnan, P. R. China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, P. R. China & School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand & 6371105004 @ lamduan. mfu. ac. th; https: // orcid. org / 0000 - 0003 - 0977 - 6414 6371105004@lamduan.mfu.ac.th Author Tibpromma, Saowaluck 0000-0002-4706-6547 Centre for Mountain Futures, Kunming Institute of Botany, Kunming 650201, Yunnan, P. R. China & CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, P. R. China & saowaluckfai @ gmail. com; https: // orcid. org / 0000 - 0002 - 4706 - 6547 saowaluckfai@gmail.com Author Karunarathna, Samantha C. 0000-0001-7080-0781 Centre for Mountain Futures, Kunming Institute of Botany, Kunming 650201, Yunnan, P. R. China & CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, P. R. China & samanthakarunarathna @ gmail. com; https: // orcid. org / 0000 - 0001 - 7080 - 0781 samanthakarunarathna@gmail.com Author Thiyagaraja, Vinodhini 0000-0002-8091-4579 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50002, Thailand & Depatment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200 Thailand & vino. thiyagaraja @ gmail. com; https: // orcid. org / 0000 - 0002 - 8091 - 4579 vino.thiyagaraja@gmail.com Author Xu, Jianchu 0000-0002-2485-2254 Centre for Mountain Futures, Kunming Institute of Botany, Kunming 650201, Yunnan, P. R. China & CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, P. R. China & jxu @ mail. kib. ac. cn; https: // orcid. org / 0000 - 0002 - 2485 - 2254 jxu@mail.kib.ac.cn Author Jayawardena, Ruvishika S. 0000-0001-7702-4885 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand & ruvishika. jay @ mfu. ac. th; https: // orcid. org / 0000 - 0001 - 7702 - 4885 ruvishika.jay@mfu.ac.th Author Lumyong, Saisamorn 0000-0002-6485-414X Depatment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200 Thailand & Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand & Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand & scboi 009 @ gmail. com; https: // orcid. org / 0000 - 0002 - 6485 - 414 X scboi009@gmail.com Author Hyde, Kevin D. 0000-0002-3407-9517 Innovative Institute of Plant Health, Zhongkai University, Guangdong, P. R. China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand & Depatment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200 Thailand & Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand & kdhyde 3 @ gmail. com; https: // orcid. org / 0000 - 0002 - 3407 - 9517 kdhyde3@gmail.com text Phytotaxa 2021 2021-12-14 528 2 111 124 journal article 2965 10.11646/phytotaxa.528.2.4 e44d5d0a-d42f-4b00-b034-c52ac8a137f6 1179-3163 5778712 Fitzroyomyces yunnanensis L. Lu, K.D. Hyde & Tibpromma , sp. nov. ( Fig. 2 ) Index Fungorum number: IF 558662; Facesoffungi number: FoF 09896 Holotype :— CHINA . Yunnan Province : Xishuangbanna , on dead wood of coffee, 24 November 2020 , Saowaluck Tibpromma, XSBN-C5 ( HKAS 113186 , holotype ); ex-type living culture KUMCC 21-0002 . Saprobic on dead wood of coffee. Sexual morph: Apothecia 550–1000 × 230–370 μm (x̅ = 800 × 270 μm, n = 10), immersed at first, opening by entire pore at maturity, subglobose. Disc deeply cupulate, pale creamy, with whitepruinose margin. Exciple 25–50 μm wide (x̅ = 38 μm, n = 10), thick-walled, comprising hyaline cells of textura intricata , inner layer composed of hyaline cells of textura angularis . Hamathecium comprising asci and paraphyses. Paraphyses hyaline, 1–2 µm wide (x̅ = 1.8 μm), numerous, long filiform, unbranched, aseptate. Asci 100–140 × 5–10 μm (x̅ = 112 × 8 μm, n = 10), unitunicate, 6–8-spored, cylindrical to ovoid, broadest at middle, rounded apex and short stalked. Ascospores 80–120 × 2–3 μm (x̅ = 88 × 2.7 µm, n = 10), overlapping uni- to bi-seriate, fusiform, 12–18- septate, hyaline, not easily breaking into different sizes, smooth-walled, guttulate, without a mucilaginous sheath. Asexual morph : Undetermined. Culture characteristics:— Colonies on PDA reaching 35 mm diam, after 4 weeks at room temperature (20–25 ℃). Culture from above, convex with surface, dry, rough, white to pale brown, circular, towards the edge with irregular white aerial mycelia, spreading, slightly covered; reverse brown to yellowish, with radiating white mycelia. Etymology:— The specific epithet “ yunnanensis ” refers to Yunnan Province where the holotype was collected. Notes:— ITS blast result showed that Fitzroyomyces yunnanensis is 89% similar to F. cyperacearum ( MK499349 ). The results of LSU blast showed that our strain is 96% similar to Stictis sp. ( MH260319 ), while mtSSU results showed it to be 90% similar to Carestiella socia ( AY661678 ). Based on phylogenetic analyses, the new strain clustered with Fitzroyomyces and formed a well-separated clade with 100% ML, 1.00 BYPP boostrap support with the extant species of Fitzroyomyces . Fitzroyomyces yunnanensis differs from extant species of Fitzroyomyces in its larger apothecia, with a clear border between outer and inner layers in the exciple, and shorter asci and ascospores. This is the first Fitzroyomyces species reported from coffee. A synopsis of characters for sexual taxa in Fitzroyomyces is provided ( Table 2 ).