Revision of Desmodorinae and Spiriniinae (Nematoda: Desmodoridae) with redescription of eight known species Author Armenteros, Maickel 869FA865-9251-489D-B399-1BA828530C6D Centro de Investigaciones Marinas, Universidad de La Habana, Calle 16 Número 114, Playa, CP 11300, Habana, Cuba. Email: maickel @ cim. uh. cu (corresponding author) & urn: lsid: zoobank. org: author: 869 FA 865 - 9251 - 489 D-B 399 - 1 BA 828530 C 6 D Author Ruiz-Abierno, Alexei 4D8DE0FF-2CC1-4DD4-BE52-4C2E5B1527DF Centro de Investigaciones Marinas, Universidad de La Habana, Calle 16 Número 114, Playa, CP 11300, Habana, Cuba. Email: maickel @ cim. uh. cu (corresponding author) & urn: lsid: zoobank. org: author: 4 D 8 DE 0 FF- 2 CC 1 - 4 DD 4 - BE 52 - 4 C 2 E 5 B 1527 DF Author Decraemer, Wilfrida 61262451-D994-411F-8331-1EB9AFACA48C Royal Belgian Institute of Natural Sciences, rue Vautier 29, B- 1000, Brussels, Belgium. alexei @ cim. uh. cu & Biology Department, Ghent University, Ledeganckstraat 35, B- 9000, Gent, Belgium. wilfrida. decraemer @ naturalsciences. be & urn: lsid: zoobank. org: author: 61262451 - D 994 - 411 F- 8331 - 1 EB 9 AFACA 48 C text European Journal of Taxonomy 2014 2014-09-15 96 1 32 journal article 21940 10.5852/ejt.2014.96 e66f3a54-e3b7-401f-9e4b-19ed26462e99 2118-9773 3838898 21EC0039-C374-47FA-AA88-46954CC7C5BD Spirinia parasitifera ( Bastian, 1865 ) Figs 11 , 12 , Table 2 Spira parasitifera Bastian, 1865: 159 . Spiliphera oxycephala Bütschli, 1874: 47 . Spirinia nidrosiensis Allgén, 1933: 60 . Fig. 11. Spirinia parasitifera ( Bastian, 1865 ) . A . Anterior region of a juvenile. B . Posterior region of ♂. Table 2. Mean (range) of morphological measures for three nematode species of the subfamily Spiriniinae from the Punta Francés coral reef, Cuba. N = number of specimens, J = juvenile. For each variable the first row corresponds to males, the second to females and the third to juveniles. Abbreviations: a.b.d. = anal body diameter; Amp. Fovea c.b.d. = relation of amphidial fovea diameter to corresponding body diameter; de Man ratios are the relation of body length to body diameter (a), to pharynx length (b) and to tail length (c) respectively; c´ = relation of tail length to anal body diameter, V % = distance (relative to body length) of vulva to anterior end.

Species/Variable	Chromaspirina inaurita	Paradesmodora immersa	Spirinia parasitifera
N	3 ♂♂, 5 ♀♀, 8 J	2 ♂♂, 4 J	2 ♂♂, 2 ♀♀, 3 J
Body length (µm)	2380 (2110–2592) 2393 (1825–2701) 1226 (694–1744)	1753 (1424–2081) – 914 (277–1635)	2712 (1854–3570) 3300 (2803–3796) 1151 (1007–1307)
de Man ratio a	59 (59–60) 53 (49–55) 39 (24–50)	47 (37–58) – 30 (13–61)	39 (29–49) 43 (41–45) 28 (27–30)
de Man ratio b	19 (17–20) 20 (18–22) 12 (8–16)	14 (11–18) – 9 (5–13)	21 (18–24) 23 (21–26) 9 (8–11)
de Man ratio c	23 (23–24) 30 (21–34) 17 (12–20)	24 (22–27) – 12 (5–22)	25 (21–29) 25 (22–28) 18 (17–19)
Amp. fovea c.b.d. (%)	38 (31–43) 36 (30–52) 29 (26–33)	54 (48–59) – 41 (38–47)	36 (33–38) 37 (36–38) 42 (35–48)
Pharynx length (µm)	124 (121–129) 120 (100–129) 99 (81–119)	124 (119–128) – 96 (53 – 130)	127 (102–151) 142 (136–148) 124 (122–125)
Maximum body ø (µm)	40 (36–43) 45 (33–45) 32 (20–43)	38 (36–39) – 30 (22–44)	69 (65–73) 77 (62–92) 41 (34–46)
Tail length (µm)	101 (92–109) 81 (69–89) 78 (60–96)	75 (52–97) – 71 (51–119)	106 (88–123) 133 (130–137) 63 (60–68)
Tail length c´	2.9 (2.7–3.0) 2.6 (2.2–3.6) 2.7 (2.1–3.0)	2.6 (1.9–3.3) – 3.4 (2.0–4.3)	2.2 (2.1–2.2) 2.7 (2.6–2.8) 2.2 (2.0–2.3)
V %	55 (48–67)	–	51
Spiculum length (µm)	51 (50–52)	45 (34–55)	81 (72–89)
Gubernaculum length (µm)	24 (22–27)	19 (18–20)	28 (25–31)

Material examined 2 ♂♂ , 2 ♀♀ and 3 juveniles , deposited in the nematode collection at Centro de Investigaciones Marinas, Universidad de La Habana , CUBA . Description Cuticle finely striated in the anterior region (annuli ~ 1 μm width) but even narrower in the mid-body and tail region. Inner and outer labial sensilla papilliform (1–2 μm long), four cephalic setae (7–10 μm long) at level of amphidial fovea. Amphidial fovea cryptospiral located forward in the head (<6 μm from apex). Eight longitudinal rows of somatic setae decreasing in length from the cervical region (7–10 μm long) to papillae at mid-body and tail region. Pharynx muscular with posterior bulb and internal lining sclerotized. Cardia inconspicuous. Secretory-excretory system not observable. Tail conical, last portion without striations. Male monorchic; spicules strongly bent, with capitulum, gubernaculum a bent rod dorsally oriented; without precloacal supplements. Female didelphic, amphidelphic with reflexed ovaries, vulva a transversal aperture. Juveniles are similar to adults, except for the development of the reproductive system. Remarks S. parasitifera is a cosmopolitan species recorded from several habitats and biogeographical regions. Coles (1987) noted that the main differences among populations from widely divergent coasts ( England , Canada and USA ) were the shape of the proximal end of the spicules and the body size. He reported that specimens from warmer waters ( Florida ) had smaller body size (males: 1650–2150 µm ; females: 1650–1910 µm ) compared to specimens from temperate waters (males: 2000–3300 µm ; females: 2020–3180 µm ). However, our specimens from a tropical area (Caribbean Sea) had body size closer to those from colder waters (males: 1854–3570 µm ; females: 2803–3796 µm ). Therefore, a negative relationship between water temperature and body size is not plausible for this species. For some species we studied in this paper this negative relationships is supported (e.g., Acanthopharynx denticulatus , Paradesmodora immersa ) but not for other (e.g., Spirinia parasitifera , Chromaspirina inaurita ). The considerable geographic variation in the morphology of S. parasitifera suggests the existence of cryptic species within this nominal species. Fig. 12. Spirinia parasitifera ( Bastian, 1865 ) , SEM microphotographs of ♂ (A, B & C) and ♀ (D). A . Head in frontal view. B . Head in lateral view. C . Anal region with portion of protruding spicule. D . Vulva. Another rare sympatric species, belonging to the genus Spirinia , has been recorded in our samples with only two specimens. The species was characterized by the small body size (male: 686 µm ; female: 684 µm ) and two kinds of somatic setae (long: 28–35 μm and short 5–8 μm). It is closer to S. gnaigeri Ott, 1977 (Ott 1977: 134) ; however, body size of type specimens in Ott (1972) is larger (males: 860– 913 µm ; females: 836–955 µm ) and the length of both somatic setae shorter (long setae: 20–22 µm ; short setae: 4–5 µm ). However, more specimens should be examined in order to evaluate if this is really a new species of Spirinia .