Capsicum carassense (Solanaceae), a new species from the Brazilian Atlantic Forest Author Barboza, Gloria E. Author de Bem Bianchetti, Luciano Author Stehmann, Joao Renato text PhytoKeys 2020 140 125 138 http://dx.doi.org/10.3897/phytokeys.140.47071 journal article http://dx.doi.org/10.3897/phytokeys.140.47071 1314-2003-140-125 60272EC138C25DB7BACD87DA6781CE80 Capsicum carassense Barboza & Bianchetti sp. nov. Figs 1 , 2 , 3 Diagnosis. Capsicum carassense is morphologically most similar to C. mirabile Mart., but differs in its moderate to dense pubescence, narrowly elliptical to lanceolate leaf blade with acute to obtuse apices, longer calyx appendages (up to 5 mm) and larger corollas (up to 20 mm in diameter). Type. Brazil. Minas Gerais: Catas Altas, RPPN Serra do Caraca , trilha da gruta de Lourdes, apos a capelinha, 20°05'41"S , 43°28'52"W , 1386 m elev., 26 Oct 2014 (fl), J.R. Stehmann, L.L. Giacomin, G.E. Barboza & S. Knapp 6347 ( holotype [two sheets]: BHCB acc.#174038 [BHCB0019940_1!, BHCB0019940_2!]; isotypes : CORD [CORD00006968!], RB [RB 01220059, acc. # 674586!]; MBM). Description. Shrubs (0.8-) 1-2 (-3) m tall, with the main stem somewhat thick and sparsely branched, the branches dichotomous and spreading horizontally. Stems hollow, angled with ridges; young stems green, striate, moderately to densely pubescent with simple, uncinate and antrorse uniseriate 3-5 (-6)-celled eglandular trichomes 0.2-0.7 mm long, yellowish-brown when dried, the nodes green or purple; bark of older stems brown, pubescent, striate; lenticels absent. Sympodial units difoliate, the leaves geminate or the leaves solitary in the bifurcation of the branches; the leaves of a geminate pair anisophyllous in size. Leaves simple, membranaceous to chartaceous, discolourous, dark green above, paler beneath; adaxial surface moderately pubescent with simple trichomes like those of the stem, especially on the veins; abaxial surface moderately pubescent like the adaxial surface, but with less frequent glandular trichomes with a unicellular stalk and a multicellular head; major leaves with blades 6-16 cm long, 0.9-2.5 cm wide, narrowly elliptic to lanceolate; the major veins 4-6 on each side of midvein, the midvein prominent and the secondary veins obscure, the base attenuate, the margins entire and moderately pubescent, the apex acute to obtuse; petioles 0.2-0.6 cm long, moderately pubescent; the minor leaves 2.9-3.9 cm long, 0.5-0.8 cm wide, narrowly elliptic; the major veins 2-3 (-4) on each side of midvein, the base attenuate, the margins entire, moderately pubescent, the apex obtuse; petioles 0.2-0.4 cm long, moderately pubescent. Inflorescence with the flowers in fascicles of 2-4; pedicels (1.2-) 1.5-2 (-2.2) cm long, slightly angled, erect to oblique, green, geniculate at anthesis, moderately pubescent. Buds ellipsoid, cream with greenish-yellow pigmentation. Flowers 5-merous, all perfect. Calyx 1.2-1.6 mm long, 2.5-3 mm wide, cup-shaped, thin, light green to cream, the margin truncate, pubescent with abundant antrorse curved 3-5-celled eglandular trichomes and sparse short glandular trichomes with a dark elongate, multicellular head and short unicellular stalk (see Fig. 1E, F ), the calyx appendages 5, (2.5-) 3-4 (-5) mm long, green, thick, erect, cylindrical, inserted very close to the margin, with the same indument as the calyx tube. Corolla (8-) 10-12 mm long, 13-20 mm in diameter, stellate, thick, with abundant interpetalar tissue, white near the lobe margins and greenish-yellow in the middle and base without, white with 5 purple spots covering the base of the lobes and the throat with a cream centre within, lobed 1/2 or less to the base, the tube 4.5-5 mm long; pubescent in the throat and the base of the lobes with long glandular trichomes with a globose peltate unicellular head and a 2-3-celled stalk inside, the lobes 4.5-6.5 mm long, 5-8 mm wide, broadly triangular to triangular, the tips cucullate, the margins densely pubescent. Stamens subequal; filaments 2.7-3.1 (-4.1) mm long, white, glabrous, inserted on the corolla ca. 1 mm from the base, with inconspicuous auricles; anthers 1.5-1.9 mm long, elliptic, the thecae blue, the pollen whitish-cream. Ovary 1.3-1.5 mm long, ca. 1.2 mm diam., light green, subglobose to ovoid, glabrous; nectary ca. 0.3 mm high, conspicuous; style 4.3-5 (-7) mm long, white, clavate, glabrous; stigma ca. 0.2 mm long, ca. 0.7 mm wide, cream, discoid. Fruit a globose-depressed berry 6-7 mm in diameter, green when immature, yellowish-green when mature, glabrous, pungent, the pericarp hyaline with very long giant cells, the endocarp alveolate; stone cells absent; fruiting pedicels 1.8-2.5 cm long, pendent and slightly curved, slightly angled and widened at the apex; fruiting calyx ca. 4 mm in diameter, persistent, not accrescent, discoid, yellowish-green, the appendages spreading, green, fleshy and cylindrical. Seeds 7-13 per fruit, 3.5-4 mm long, 2.5-3 mm wide, ellipsoidal to reniform, brownish-black to black, the seed coat deeply reticulate, with small spine-like projections. Chromosome number not known. Figure 1. Capsicum carassense Barboza & Bianchetti A flowering branch B-D leaf morphology E eglandular trichome of the stem F glandular trichome of the calyx G, H flower buds in different stages of development I flower in anthesis (note the geniculate pedicel) J opened corolla K fruit L fruiting calyx A-K from Bianchetti et al. 1364. Drawn by L. Bianchetti. Figure 2. Capsicum carassense Barboza & Bianchetti A habit, showing the typical lanceolate leaves B inflorescence with geniculate pedicels C flower, in frontal view D globose-depressed fruit A-C from Stehmann 6347 D from Agra 7268. Photos by J.R. Stehmann. Scale bars: 2 cm ( A ); 1 cm ( B ); 5 mm ( C-D ). Distribution. Capsicum carassense is endemic to south-eastern Minas Gerais (Fig. 3 ), growing mainly in the Serra do Caraca and other nearby mountainous areas (Serra do Gandarela, Serra Geral, Serra do Capanema, Serra Sao Geraldo), between 1000-1390 m elevation. Figure 3. Map showing the distribution of Capsicum carassense (triangle) and C. mirabile (square) in south-eastern Brazil. Colour map was reproduced and adapted from Google Earth Pro. Abbreviations. MG, Minas Gerais; ES, Espirito Santo; RJ, Rio de Janeiro; SP, Sao Paulo. Ecology. The population studied in the field at Serra do Caraca inhabits the understorey of the semi-deciduous montane Atlantic Forest, in a shaded and moist environment. Information about pollination and dispersal is not yet known. Phenology. In flower from October to January, also in May; fruiting in December, February and April. Etymology. The new species is named in allusion to its restricted habitat in the Serra of Caraca and surrounding areas (Minas Gerais, Brazil). Preliminary assessment of conservation status. Following the IUCN Red List Criteria ( IUCN 2019 ), this species is considered Endangered (EN) B2 a,b (iii, iv). We suggest this category, because of the species' very restricted geographic distribution (EOO <483.4 km2, AOO <32 km2), as well as the increasingly degraded habitat quality, especially associated with the extensive iron mining activities in the region (see below). Specimens examined. Brazil . Minas Gerais : Mun. Catas Altas, Serra do Caraca , near Santa Barbara, trilha a Capela y Gruta de Lourdes, 20°05'39"S , 43°28'45"W , 1430 m elev., 26 Apr 2010 (fr), M.F. Agra et al. 7268 (BHCB, JPB, RB, UT); Serra do Caraca , ca. 70 km sudeste de Belo Horizonte, proximo ao Mosteiro do Caraca , 17 Nov 1977 (fl), N.D. da Cruz et al. 6291 (SP, RB, UEC); Barao de Cocais, Parque Natural do Caraca (PNC), a 150 m do monasterio , perto da Fonte do Bode, 1250 m elev., 19°56'S , 43°28'W , 22 Apr 1986 (fr), L. Bianchetti et al. 512 (CEN); Barao de Cocais, PNC, 31 May 1992, L. Bianchetti et al. 1363, 1364, 1367, 1368 (CEN); PNC, muy cerca del monasterio, en el bosquecillo vecino al Fonte de Bode, ca. 1250 m elev., 21 Apr 1986 (fr), A.T. Hunziker et al. 25206 (CORD); PNC, muy cerca del monasterio, 1250-1300 m elev., 10 Dec 1986 (fl), A.T. Hunziker et al. 25256 (CORD, BM); Serra do Caraca , 4 Dec 1999 (fl), R.C. Mota 106 (BHCB); at the same locality, 18 Dec 2002 (fl), R.C. Mota 2260 (BHCB); at the same locality, 5 Jan 2005 (fl), R.C. Mota 2653 (BHCB); RPPN Caraca , Trilha para Tanque Grande, 03 Dec 2013 (fr), J. Ordones et al. 2229 (BHZB); Serra do Caraca , 1600 m elev., 12 Sep 1990 (fl), J.R. Stehmann et al. s.n. (ESA 33757); Serra do Caraca , Tanque Grande, 16 Feb 2012 (fr), J.R. Stehmann & F.S. Faria 6269 (BHCB); na trilha para o Tanque Grande, 20°06'05"S , 43°29'33"W , 1249 m elev., 26 Oct 2014 (fl), J.R. Stehmann et al. 6344 (BHCB, RB); Mun. Ouro Preto, Mina da Capanema (Mina da Serra Geral), RPPN Vale, near town of Glaura, trail to Cabeza do Macaco, 20°13'04"S , 43°35'05"W , 1691 m elev., 29 Apr 2011 (fr), M.F. Agra et al. 7340 (BHCB); estrada da torre-Samarco Mineracao-Antonio Pereira, 16 Dec 1996 (fl, fr), M. Brosehel & J. Craig 406 (RB); RPPN Capanema, 19 Oct 2015 (fl), M.O. Pivari et al. 2768 (BHCB); Mun. Santa Barbara, Serra de Gandarela/C2, 20°3'24"S , 43°41'28.60"W , 1637 m elev., 26 Nov 2008 (fl), F.F. Carmo & L.C. Ribeiro 3527 (BHCB); Santa Barbara, 20°00'52"S , 43°40'13"W , 1497 m elev., 17 Dec 2014 (fl), F.D. Gontijo et al. 589 (BHCB); Rio Acima, Serra do Gandarela, 20°05'52"S , 43°41'12"W , 12 Dec 2011 (fl), C.V. Vidal & R.L. de Paula 1157 (BHCB); Without municipality: habitat in irriguis lapidosis Serra do S. Geraldo, w/d, C.F.P. von Martius s.n. (M 0171537). Discussion. Capsicum carassense belongs to the Atlantic Forest clade (sensu Carrizo Garcia et al. 2016 , as Capsicum aff. mirabile ). For many years, this species has long been confused with C. mirabile in herbaria ( Bianchetti 1996 , Barboza per. obs.). Both species share similar traits, such as habit, geniculate pedicels at anthesis, number of calyx appendages, the shape and colour of the corolla, colour and pungency of the fruits and blackish seeds. They can be easily distinguished by the indumentum, shape of the major leaf and its length/width ratio, length of the calyx appendages, corolla size and ecology and distribution (see Table 1 for contrasting details). Table 1. Differences between C. carassense and C. mirabile . Abbreviations. BA: Bahia, ES: Espirito Santo, RJ: Rio de Janeiro, SP: Sao Paulo, MG: Minas Gerais.

Character	Capsicum carassense	Capsicum mirabile
Indumentum	Moderately to densely pubescent	Glabrate to sparsely pubescent
Major leaf shape	Narrowly elliptical to lanceolate, apex acute to obtuse	Elliptical to ovate, rarely narrowly elliptical, apex acuminate to long acuminate
Major leaf length/width ratio	(4-) 5-10 (-16)	(2-) 2.5-4 (-4.9)
Buds colour	Cream with greenish-yellow tones	Purple
Calyx appendages	Long appendages (2.8-) 3-4 (-5) mm	Short to long appendages
	-	(0.4-) 0.5-1.5 (-3) mm
Corolla size	(8-) 10-12 mm long	(6-) 7.5-12 mm long
	13-20 mm in diameter	(9-) 10-13 mm in diameter
Distribution and ecology	Endemic to Serra do Caraca and surrounding areas (MG); mostly in semi-deciduous montane forests	Widely distributed in eastern and southern Brazil (BA, ES, RJ, SP, MG); mostly in dense ombrophilous montane forests

Capsicum carassense is a pubescent low shrub with very narrow leaves and large white corollas with purple-spots. The shape and length/width ratio of the leaves of C. carassense are very close to the description of C. mirabile var. grandiflorum Sendtn. ( Sendtner 1846 ) but Sendtner also stated that this variety had "floribus majoribus" and "planta glaberrima". The diameter of the corolla measured in three flowers in the F neg. 2871 of the destroyed varietal holotype (Sellow 209, Herb. Reg. Berolinense) is not more than 1 cm (https://collections-botany.fieldmuseum.org/project/6454), thus these two traits, size of the corolla and lack of pubescence fit with the concept of C. mirabile rather than C. carassense . Capsicum carassense and C. mirabile differ in geographic distribution, with the former inhabiting mostly the understorey of the semi-deciduous montane forests of the southernmost areas of the Espinhaco Range in Minas Gerais, while the latter has a wider distribution ( Barboza and Bianchetti 2005 ), growing mostly along the dense ombrophilous montane forest of south-eastern Brazil, an area characterised by high rainfall and humidity and the absence of a pronounced dry season ( Silva Magnago et al. 2007 ). Both species have a contact zone at the municipalities of Mariana and Ouro Preto, in the Serra do Itacolomi, where C. carassense , as well as two other species, C. mirabile and C. villosum Sendtn., were recorded. There is no information about edaphic preferences for these species, nor possible events of hybridisation in this contact area. All collections of the new species come from the Iron Quadrangle in Minas Gerais, except one historical Martius specimen at M [M!, photo n° 6522 at F!], collected in the Serra de Sao Geraldo between Mariana and Presidio de Sao Joao Batista (today the municipality of Visconde do Rio Branco). This material, a syntype of C. mirabile ( Sendtner 1846 ), was examined when Barboza (2011) lectotypified C. mirabile . She stated that "the third syntype [ ... ] is unusually pubescent for this species". Here, we re-examined this specimen housed at M (M-0171537) and concluded that it actually belongs to C. carassense . The relationships amongst the species belonging to the Atlantic Forest clade appear to be fully resolved and an apparent phase of rapid speciation has been suggested for this lineage ( Carrizo Garcia et al. 2016 ). In spite of the morphological similarity between C. carassense and C. mirabile , these species are not closely related phylogenetically, as C. carassense (as Capsicum aff. mirabile in Carrizo Garcia et al. 2016 ) is not sister to C. mirabile , but occurs in a different subclade of the Atlantic Forest clade. In the analysis of Carrizo Garcia et al. (2016) , C. mirabile appears closest to C. villosum var. muticum Sendtn. The new species deserves conservation attention, because few populations are known and most of them are distributed in the Iron Quadrangle and associated with remnants of native forests. This area was assessed as priority for conservation in the state of Minas Gerais ( Drummond et al. 2005 ), with high animal and plant diversity and extensive threats, especially from iron mining activities ( Jacobi et al. 2011 , Salles et al. 2018 ). The impacts on native vegetation, especially forests, are high because of the activities associated with iron and bauxite mining as the building of dams and urban expansion, all increase deforestation pressures at regional-scale ( Sonter et al. 2014 ).