Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes) Author Zhang, Zhi-Yuan https://orcid.org/0000-0003-2031-7518 Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang 550025, China & College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang 550025, China Author Li, Xin https://orcid.org/0000-0001-7910-1469 Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang 550025, China Author Chen, Wan-Hao https://orcid.org/0000-0001-7240-6841 Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China Author Liang, Jian-Dong https://orcid.org/0000-0002-3939-3900 Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China Author Han, Yan-Feng https://orcid.org/0000-0002-8646-3975 Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang 550025, China swallow1128@126.com text MycoKeys 2023 2023-06-29 98 167 220 http://dx.doi.org/10.3897/mycokeys.98.102816 journal article http://dx.doi.org/10.3897/mycokeys.98.102816 1314-4049-98-167 A8196420B4CC5D87B89C0AB494A018C9 Aspergillus doliiformis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov. Fig. 5 Etymology. Referring to the lantern shape of conidia. Type . Hainan Province, Haikou City, Haidian Campus of Hainan University 20°05'76"N, 110°32'91"E, from soil, 28 Aug 2019 , Z.Y. Zhang (HMAS 351867 holotype designated here, ex-type living culture CGMCC 3.20772 = GZUIFR 21.885); ibid., GZUIFR 21.883. Figure 5. Aspergillus doliiformis (from ex-holotype CGMCC 3.20772) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-k phialides and conidia. Scale bars: 10 µm ( d-k ). Description. Culture characteristics (14 d at 25 °C): Colony on PDA 23-26 mm diam., white (1A1), felty, fluffy, margin slightly undulate; reverse pale yellow (1A3) to white (1A1) from centre to margin. Colony on MEA 16 mm diam., white (1A1) at margin, olive yellow (2D8) at centre, felty, margin entire; reverse oak brown (5D6) to brownish-grey (5D2) from centre to margin. Colony on OA 26-27 mm diam., white (1A1), flocculent, margin entire, producing a diffusible faint yellow pigment; reverse greyish-yellow (2B4). Hyphae hyaline, septate, smooth, branched, 1.0-3.5 μm wide. Conidiophores solitary phialides borne laterally or terminally on vegetative hyphae. Phialides mono- to polyphialidic, hyaline, ampulliform, campaniform, cylindrical or tapering with an enlarged base, sometimes curved irregularly, occasionally branched, neck cylindrical or broadly tapering, sometimes extending sympodially from the necks, 2.5-18.0 x 1.0-5.0 µm . Conidia borne solitary or formed in long chains, hyaline, smooth or roughened, lantern, subglobose to globose, obpyriform, 3.0-5.0 x 2.5-4.5 µm (av. 4.2 x 3.4 μm , n = 50). Sexual morph not observed. Additional specimens examined. China : Hainan Province , Haikou City , Hainan General Hospital 20°00'57"N , 110°28'78" E, from soil, 28 Aug 2019 , Z.Y. Zhang , GZUIFR 21.884; Haikou People's Park , soil, 28 Aug 2019 , Z.Y. Zhang , GZUIFR 21.886 . Notes. Aspergillus doliiformis represents a new lineage within the subgenus Polypaecilum Polypaecilum , series Canini , forming a fully supported clade (ML = 100%; PP = 1.0). A. doliiformis is phylogenetically closely related to A. limoniformis and A. cylindricus . However, A. doliiformis can be distinguished from A. limoniformis by their sequence similarity (93% 502/539; 99% 847/853; 87% 370/423; 91% 762/834; 92% 724/791 similarity of ITS, LSU, TUB , RPB2 and TSR1 in A. limoniformis CGMCC 3.19323). Morphologically, the conidia of A. limoniformis are limoniform or subglobose, rather than lantern, subglobose to globose, obpyriform in A. doliiformis ( Zhang et al. 2021a ). In addition, the distinction between A. doliiformis and A. cylindricus is shown in the notes of A. cylindricus .