Reconciliation between neontology and paleontology in the Gryllidea (Orthoptera, Ensifera): reinterpreting the venation of the stridulatory apparatus in crickets Author Josse, Hugo Institut de Systématique, Evolution, Biodiversité (ISYEB) Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, case postale 53, 57 rue Cuvier, F- 75231 Paris cedex 05 (France) & Géosciences Rennes, UMR 6118, Université de Rennes, 35000 Rennes (France) hugojosse98@gmail.com Author Faberon, Léo Institut de Systématique, Evolution, Biodiversité (ISYEB) Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, case postale 53, 57 rue Cuvier, F- 75231 Paris cedex 05 (France) Author Schubnel, Thomas Institut de Systématique, Evolution, Biodiversité (ISYEB) Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, case postale 53, 57 rue Cuvier, F- 75231 Paris cedex 05 (France) Author Nel, André Institut de Systématique, Evolution, Biodiversité (ISYEB) Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, case postale 53, 57 rue Cuvier, F- 75231 Paris cedex 05 (France) Author Desutter-Grandcolas, Laure Institut de Systématique, Evolution, Biodiversité (ISYEB) Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles, case postale 53, 57 rue Cuvier, F- 75231 Paris cedex 05 (France) text Zoosystema 2023 2023-12-13 45 24 769 801 https://sciencepress.mnhn.fr/sites/default/files/articles/pdf/zoosystema2023v45a24.pdf journal article 10.5252/zoosystema2023v45a24 1638-9387 10376749 8D29DD39-7ADC-4B36-8E95-3A3F930301D6 FOREWING VENATION PATTERN IN GRYLLOTALPIDAE According to our hypothesis, the venation of the lateral field of mole crickets is almost the same as in the crickets s. str. and fossil families. Indeed, even if their bases are very close, we can identify the C, Sc, R and M+CuA ( Fig. 7 ). Lateral field. In the proximal half of the wing, Sc, R and M+CuA veins are almost parallel to each other. The C is fused basally with the Sc, and is composed of several slender branches directed anteriorly. Sc has the same branching shape as in Grylloidea , and we also do not distinguish between ScA and ScP in mole crickets. R, posterior to Sc, is strong and convex. M+CuA is the most posterior vein of the lateral field. Four distal branches are usually present in the lateral field and in the so-called ‘flexible zone’ ( Fig. 7 ): The two most anterior veins are identified as RA and RP, while the other two may be MA and MP. RA and RP are both strong, but the RP appears to be slightly concave compared to the very convex RA. MA is clearly stronger than MP and convex, while MP is concave. Just at the base of the flexible zone, a small cell is visible. According to our hypothesis, this small cell could be homologous to the lanceolate cell of Grylloidea and fossil taxa ( Fig. 8 ). This interpretation necessitates numerous vein reorganisation (i.e., change of vein polarity and thickness), but it is well-supported by similarities between this cell and the lanceolate cell of fossil crickets (as in † Angarogryllus angaricus , Fig.8A ), as shown by: the presence of a curved crossvein r-m between R and M; the presence of a fold crossing part of M+CuA after its point of contact with r-m and then crossing this cell; the relative positions of the veins that define the lanceolate cell of crickets; and the relative convexities of the four distal veins of this area (RA/RP and MA/MP). According to this scheme, r-m would have been reinforced in the continuity of the M+CuA in some species as Scapteriscus sp. ( Fig. 8C ), while the weak transverse vein that borders basally the lanceolate cell would be the ‘real’ M+CuA. But this pattern is not present in all Gryllotalpidae : for example, in Gryllotalpa sp. ( Fig. 8B ), r-m is slightly oblique (as in some † Protogryllidae ) and weaker than the main veins (except the part of M+CuA that forms the base of the lanceolate cell, which is as weak as in Scapteriscus sp. ). In fact, the polarity and orientation of the veins delimiting this cell are variable according to the species, making the identification of the vein bordering distally the lanceolate cell very complicated: it will be called here ‘r-m2’ because of its relative position ( Fig. 8 ), but we are aware of three possible interpretations for these veins. A first hypothesis would consider this very strong and convex ‘r-m2’ as another anterior branch of MA, but this would imply a change in convexity distally, when this branch merges with RP (because RP+MA would be concave). Another hypothesis is that ‘r-m2’ is the result of the capture of the base of RP (before its fusion with MA, see in † Angarogryllus angaricus , Fig. 8A ) by the very distal part of RP (after its separation with MA, Fig. 8A ), a scheme that could apply to some Grylloidea (like Phyllogryllus sp. , see Fig. 4B ). Finally, r-m2 could just be a reinforced crossvein between R and M. Clearly, the origin of the ‘r-m2’ vein cannot be determined with certainty according to our observations. FIG . 5. — Hypothesis of primary venation homology in male Grylloidea with particular forewing venation: A , B , species with ‘shortened’ wings; B , C , species with ‘reduced’ stridulatory apparatus. A , Landreva sp. (MNHN-EO-ENSIF9775, Gryllidae ); B , Nemobius sylvestris (Bosc, 1792) (MNHN-EO-ENSIF9786, Trigonidiidae ); C , Tafalisca lineatipes Bruner,1916 (MNHN-EO-ENSIF9760, Oecanthidae ); D , Aphonomorphus sp. (MNHN-EO-ENSIF9764, Oecanthidae ).Abbreviations: ‘ha’, distally opened harp; ‘mi’ distally opened mirror; others and colour code: see text. Grey dash lines represent folds. Scale bars: 1 mm. FIG . 6. — Hypothesis of primary venation homology of male forewing of † Protogryllidae ( A , B ) and † Baissogryllidae ( C -E ): A , † Angarogryllus angaricus ( Sharov 1968 ) , PIN 1873-16; B , † Falsipseculum karatavicum ( Sharov 1968 ) , PIN 3791/1345; C , † Neosharategia paradoxa Gorochov, 1992 , PIN 4270-210a; D , † Baissogryllidae sp., CCNH-293; E , † Anglogryllus lyristes Gorochov et al. , 2006 ,MNEMG 2003.46. Abbreviations: “ha” , distally opened harp; “mi” , distally opened mirror, others and colour code, see text. Grey dash lines represent folds. Scale bars: 1 mm. FIG . 7. — Hypothesis of primary venation homology of forewing of male Scapteriscus sp. MNHN-EO-ENSIF3069 ( Gryllotalpidae ).Abbreviations and colour code: see text. Grey dash lines represent folds. Scale bar: 1 mm. As in crickets, mole crickets have a median fold between M+CuA and CuPa ( Fig. 8 ). This fold is clearly less marked than in the Grylloidea . We can also notice that two distal folds border the flexible zone: one crossing the lanceolate cell and the other running along CuA+CuPaα. Dorsal field. From the most anterior to the most posterior, the following veins are present: CuPa, CuPb, a simple PCu, AA and AP ( Fig. 8 ). CuPa begins with a more or less straight trajectory oriented toward the distal margin; it then curves slightly posteriorly before dividing into an anterior CuPaα and a posterior CuPaβ. CuPaα merges with the CuA at its base. In the observed specimens, CuA+CuPaα is simple. CuPaβ is oblique and bent proximally on its first part, then curves sharply to orient distally and begins a trajectory parallel to the CuA+CuPaα ( Fig. 8 ). An oblique and strongly reinforced secondary vein d2 is in continuity with the base of CuPaβ and connects it to the anal node ( Fig. 8 ). CuPb is very blurred at its base, although it originates from the same basivenal bulla as CuPa (see Desutter-Grandcolas et al. 2017 ). CuPb is strongly curved at right angle and bears teeth on its ventrally side (= stridulatory file). A strong longitudinal vein identified as a secondary vein connects CuPa with CuPb in the harp. On several mole cricket specimens that we observed (as in Scapteriscus sp , Fig. 7 ; Appendix 3 : Fig. S7C ), we noticed the presence of a short vein that we identify tentatively as a ‘cup spl’ (see Discussion). A large cell is present between d2 and CuPb, posterior to the harp; it is not homologous to the harp, nor to the mirror of the Grylloidea , because it is not delimited by the same veins. We propose to name it the subharp cell. The PCu has a characteristic curved and rounded base. CuPb and PCu merge at the plectrum level, before separating distally to the plectrum and running rather parallel to the longitudinal axis of the wing ( Fig. 8 ). The AA vein is often well-differentiated from the base, while AP sometimes forms a network of small undifferentiated veins at its base. The anal veins fuse at the level of the plectrum before dividing again distally and running parallel to the longitudinal axis of the wing. FIG . 8. — Hypotheses of primary venation homology of forewing of A , † Angarogryllus angaricu s ( Sharov 1968 ) (PIN 1873-16, † Protogryllidae , cf fig. 6A); B , Gryllotalpa sp. (MNHN-EO-ENSIF3938, Gryllotalpidae ); C , Scapteriscus sp. (MNHN-EO-ENSIF3068, Gryllotalpidae ). Abbreviations and colour code: see text. Scale bars: 1 mm. In the distal half of the dorsal field, between the CuA+CuPaα and the posterior margin, vein configuration is highly variable among the species, or even between the two wings of the same species, or the two wings of the same individual (as in Gryllotalpa sp. , Appendix 3 : Fig. S7A, B ). Additional veins, which we consider as intercalary secondary veins, may even be inserted between the main veins ( Fig. 8 ). However, if vein fusions or branching may vary in this area, all these veins are generally parallel to each other and run parallel to the longitudinal axis of the wing.