Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China Author Li, Wen-Li School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China Author Liang, Rui-Ru https://orcid.org/0000-0001-7727-0998 School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China Author Dissanayake, Asha J. https://orcid.org/0000-0002-8061-8884 School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China Author Liu, Jian-Kui https://orcid.org/0000-0002-9232-228X School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China ljiankui@gmail.com text MycoKeys 2023 2023-05-23 97 71 116 http://dx.doi.org/10.3897/mycokeys.97.103118 journal article http://dx.doi.org/10.3897/mycokeys.97.103118 1314-4049-97-71 6292387F797752B1AE9167726B3A8D11 Diplodia seriata De Not., Micr. Ital. Dec. 4: 6. (1942). Fig. 11 Description. Saprobic on decaying branches of Camellia oleifera . Sexual morph: Ascomata 301-343 x 293-340 ( x̄ = 322 x 316 μm , n = 10), more or less subglobose, solitary or gregarious, semi-immersed, medium brown to dark brown, unilocular, papillate, ostiolate. Ostiole 72-78 μm diam., conical or circular, central, papillate, periphysate. Peridium 33-44 μm wide, composed of dark brown, 4-6 layers of textura angularis . Pseudoparaphyses 2-2.5 μm wide, hyaline, branched, septate. Asci 112-141 x 27.5-30 μm ( x̄ = 126 x 28.5 μm , n = 30), clavate, stipitate, bitunicate, containing eight, biseriate ascospores. Ascospores 31.5-32.5 x 12-13.5 μm ( x̄ = 32 x 13 μm , n = 30), L/W ratio = 2.5, broadly fusiform to oval, widest in the middle, both ends obtuse, hyaline, moderately thick-walled, smooth, becoming brown when aged. Asexual morph : Not observed. Figure 11. Diplodia seriata (HUEST 22.0073, new host record) a-c appearance of ascomata on natural substrate d vertical section of ascomata e section of peridium f-i asci j-n ascospores. Scale bars: 100 μm ( d ); 10 μm ( e, j-n ); 20 μm ( f-i ). Culture characteristics. Ascospores germinate on PDA within 12 h. Colonies growing on PDA, reaching a diameter of 4 cm after five days at 25 °C, effuse, velvety, with entire to slightly undulate edge. Material examined. China , Sichuan Province , Jiangyou City , shuanghe County , 31°54'10"N , 104°55'57"E , elevation 656 m , on dead branches of Camellia oleifera , 10th June 2021 , W.L Li , 288 (HUEST 22.0073), living culture UESTCC 22.0072 . Notes. The morphology of the taxa isolated from decaying woody oil plants is similar to Diplodia seriata . In the multi-gene phylogenetic analysis, our new collection clustered with the ex-type strain of Di. seriata (CBS 112555) with strong bootstrap support. Diplodia seriata has been isolated from a wide range of hosts (121 species) and has a worldwide distribution (reported in 46 countries) ( Batista et al. 2021 ). This is the first report of Di. seriata isolated from Camellia oleifera .