Barbatula leoparda (Actinopterygii, Nemacheilidae), a new endemic species of stone loach of French Catalonia
Author
Gauliard, Camille
Author
Dettai, Agnès
Author
Persat, Henri
Author
Keith, Philippe
Author
Denys, Gaël P. J.
text
Cybium
2019
43
2
169
177
http://dx.doi.org/10.26028/cybium/2019-423-005
journal article
10.26028/cybium/2019-423-005
2101-0315
12711136
8616356D-5FE1-4807-8856-217CBA972DA3
Barbatula leoparda
n. sp.
(
Figs 1-3
;
Tab. I
)
Material examined
Holotype
. –
MNHN 2018-0228,
62.6 mm
SL
♀
,
France
: Dept. Pyrénées-Orientales:
Têt River
at
Nefiach
:
N 42.697
E 2.663
,
7 Sep. 2016
, Denys and ONEMA coll.;
Paratypes
. –
MNHN 2016-0347, 4,
32.4 mm
SL
♂
,
36.84 mm
SL
♂
,
44.5 mm
SL
♂
,
61 mm
SL
♂
, collected with holotype; MNHN 2010-0997, 1,
48.2 mm
SL
♂
,
France
: Dept. Pyrénées-Orientales:
Tech River
at
Elne
:
N 42.584
E 2.970
,
14 Sep. 2010
, Denys and ONEMA coll.; MNHN erage of 218, minimum coverage 164)), genseq-1 complete 12S rDNA (GenBank accession MK518372, 950 bp, average coverage of 305, minimum coverage 93))
.
Figure 1. – Specimens of
Barbatula
spp.
in lateral view.
A
: Holotype of
Barbatula leoparda
MNHN 2018
-0228, 62.6 mm SL ♀, Têt River at Nefiach, 7 Sep. 2016, Denys and ONEMA coll.;
B
: Paratype of
Barbatula leoparda
n. sp.
MNHN 2016-0351, 46.5 mm SL ♀, Tech River at Céret, 9 Sep. 2016, Denys, Hautecoeur and ONEMA coll;
C
:
Barbatula barbatula
MNHN 2013
-0826, 72.9 mm SL ♂, stream Clauge (Rhône drainage) at La Loye, 1 Jul. 2013, Denys and ONEMA coll.;
D
: Holotype of
Barbatula quignardi
MGAB
77, 52 mm SL ♂, Lez River, Jul. 1962, Băcescu coll.
Figure 2. – Specimens of
Barbatula
spp.
in ventral view.
A
: Holotype of
Barbatula leoparda
MNHN 2018
- 0228, 62.6 mm SL ♀, Têt River at Nefiach, 7 Sep. 2016, Denys and ONEMA coll.;
B
:
Barbatula barbatula
MNHN 2013
-0826, 72.9 mm SL ♂, stream Clauge (Rhône drainage) at La Loye, 1 Jul. 2013, Denys and ONEMA coll.;
C
: Holotype of
Barbatula quignardi
MGAB
77, 52 mm SL ♂, Lez River, Jul. 1962, Băcescu coll.
Figure 3. – Mouth of holotype of
Barbatula leoparda
MNHN 2018
- 0228, 62.6 mm SL ♀, Têt River at Nefiach, 7 Sep. 2016, Denys and ONEMA coll. Abbreviations: medial indentation of upper lip (mi), mental lobe of lower lip (ml). Scale bar = 1 mm.
2016-0351, 1,
46.5 mm
SL
♀
,
Tech River
at
Céret
:
N 42.496
E 2.755
,
9 Sep. 2016
, Denys, Hautecoeur and ONEMA coll
.
Material for molecular references
Holotype
. –
MNHN 2018-0228,
France
: Dept. Pyrénées-Orientales:
Têt River
at
Nefiach
:
N 42.697
E 2.663
, genseq-1 COI (GenBank accession MK518369, 650 bp, average cov-
Diagnosis
Barbatula leoparda
larger than
47 mm
SL is distinguishable from
B. barbatula
of the same size by the presence of dark-brown blotches on the belly and the jugular area (
vs.
absence of pigmentation) (
Fig. 2
), a larger interorbital width (35.6 to 41.8% HL,
vs.
23.0 to 38.1% HL;
Fig. 4A
), a deeper caudal peduncle (12.1 to 13.0% SL,
vs.
8.0 to 12.4% SL;
Fig. 4C
), and a fewer ratio length/depth of the caudal peduncle (1.30 to 1.48,
vs.
1.38 to 2.56;
Fig. 4D
). Sub-adult specimens (
36 to 47 mm
SL) are diagnosable by a larger interorbital width (35.5 to 40.5% HL,
vs.
28.7 to 38.4% HL;
Fig. 4B
), a fewer ratio length/depth of the caudal peduncle (1.41 to 1.45,
vs.
1.45 to 2.15;
Fig. 4E
) and longer pelvic fins (15.8 to 16.5% SL,
vs.
12.4 to 15.5% SL;
Fig. 4G
).
Barbatula leoparda
larger than
47 mm
SL is distinguishable from
B. quignardi
of the same size by the presence of dark-brown blotches on the belly and the jugular area (
vs.
absence of pigmentation) (
Fig. 2
), a larger interorbital width (35.6 to 41.8% HL,
vs.
21.6 to 36.0% HL;
Fig. 4A
) and a shorter dorsal-fin length (17.1 to 20.1% SL,
vs.
18.9 to 23.9% HL;
Fig. 4F
). Subadult specimens are diagnosable
Description
Table I. – Morphometry of
Barbatula leoparda
,
B. barbatula
and
B. quignardi
for the two sizes classes (36 to 47 mm SL and> 47 mm SL). Values in parentheses: mean. Values of holotype are included in range. Bold mean values highlight morphometric differences (see Fig. 4).
|
Barbatula leoparda
n. sp.
|
Barbatula barbatula
|
Barbatula quignardi
|
| 36-47 mm SL |
> 47 mm SL Holoype |
36-47 mm SL |
> 47 mm SL |
36-47 mm SL |
> 47 mm SL |
| N |
3 |
3 |
8 |
37 |
15 |
52 |
| Standard length SL (mm) |
36.6-46.5 (42.6) |
48.2-62.6 (57.3) 62.6 |
37.8-46.9 (41.5) |
47.1-81.5 (63.6) |
36.4-46.8 (42.4) |
47.8-77.6 (59.8) |
|
In percent of SL
|
| Head length HL |
22.8-23.4 (23.1) |
22.1-23.4 (22.7) 22.1 |
21.7-25.0 (23.8) |
20.6-24.9 (22.7) |
22.4-26.7 (24.5) |
19.8-26.5 (23.2) |
| Predorsal length |
50.4-53.7 (52.3) |
50.5-53.8 (52.2) 52.3 |
51.4-54.4 (53.3) |
50.6-55.8 (52.7) |
49.5-56.4 (52.9) |
48.7-56.0 (52.5) |
| Postdorsal length |
31.7-37.7 (35.6) |
34.1-38.6 (35.6) 34.1 |
33.0-38.1 (36.5) |
33.9-42.8 (38.1) |
32.8-39.4 (36.1) |
32.7-39.6 (36.5) |
| Prepectoral length |
22.7-26.4 (24.4) |
22.8-24.8 (23.6) 22.8 |
23.7-28.0 (25.7) |
19.7-27.6 (23.3) |
21.1-29.1 (24.9) |
20.4-26.4 (23.8) |
| Prepelvic length |
53.4-56.2 (55.2) |
51.1-56.1 (53.7) 56.1 |
51.9-58.0 (54.9) |
49.1-61.6 (53.5) |
51.9-57.8 (54.3) |
50.9-56.7 (53.9) |
| Preanal length |
76.3-80.3 (78.5) |
74.0-79.5 (76.7) 76.8 |
74.1-79.6 (76.7) |
74.1-80.1 (76.1) |
75.4-83.1 (77.8) |
74.7-79.6 (77.1) |
| Caudal peduncle length CPL |
16.6-17.9 (17.2) |
17.0-17.8 (17.3) 17.0 |
15.0-20.2 (17.5) |
15.0-20.6 (17.7) |
13.1-20.4 (16.7) |
14.2-19.7 (17.3) |
| Caudal peduncle depth CPD |
11.7-12.3 (12.1) |
12.1-13.0 (12.7)
12.9
|
9.3-12.0 (10.5) |
8.0-12.4 (10.8)
|
9.5-13.2 (11.4) |
10.0-14.0 (12.2) |
| Body depth |
14.1-14.9 (14.4) |
13.4-15.8 (14.5) 14.2 |
13.8-15.8 (14.6) |
10.6-18.1 (15.0) |
13.2-16.7 (14.5) |
12.2-19.8 (15.1) |
| Pectoral-fin length |
18.9-23.0 (20.5) |
16.0-21.2 (18.4) 16.0 |
17.0-23.1 (19.3) |
13.4-23.0 (17.9) |
17.9-26.0 (20.5) |
16.0-23.9 (19.9) |
| Pelvic-fin length |
15.8-16.5 (16.3)
|
13.5-16.0 (15.1) 13.5 |
12.4-15.5 (14.5)
|
11.1-16.7 (14.0) |
13.9-18.2 (15.8) |
12.7-18.5 (15.8) |
| Dorsal-fin length |
20.9-22.7 (21.8) |
17.1-20.1 (19.0)
17.1
|
18.7-22.5 (20.2) |
16.0-24.2 (19.2) |
18.6-25.6 (21.9) |
18.9-23.9 (21.6)
|
| Anal-fin length |
15.2-17.0 (15.9) |
14.2-17.2 (15.4) 14.2 |
14.0-17.0 (15.6) |
10.0-17.9 (14.8) |
13.9-19.0 (16.6) |
13.4-19.4 (16.4) |
|
Ratio CPL/CPD
|
1.40-1.45 (1.43)
|
1.30-1.48 (1.37)
1.32
|
1.45-2.15 (1.68)
|
1.38-2.56 (1.66)
|
1.26-1.77 (1.47) |
1.14-1.71 (1.42) |
|
In percent of HL
|
| Snout length |
41.4-48.6 (44.3) |
39.5-48.3 (44.5) 45.8 |
39.7-53.8 (45.2) |
39.4-50.2 (44.3) |
36.0-47.9 (43.1) |
38.0-51.7 (44.0) |
| Eye diameter |
15.6-19.5 (17.8) |
16.0-19.3 (17.8) 18.0 |
15.9-24.6 (18.6) |
12.8-23.9 (16.3) |
14.9-21.9 (18.9) |
13.5-21.0 (17.6) |
| Postorbital length |
46.0-51.6 (48.3) |
45.7-47.0 (46.5) 46.9 |
45.7-54.2 (47.8) |
40.7-53.5 (46.9) |
38.4-48.8 (42.2) |
37.3-56.3 (43.9) |
| Interorbital width |
35.5-40.5 (37.6)
|
35.6-41.8 (38.0)
41.8
|
28.7-38.4 (33.3) |
23.0-38.1 (30.9) |
21.4-35.6 (29.4) |
21.6-36.0 (29.4) |
Figure 4. – Boxplots highlighting morphometric differences between
Barbatula leoparda
(in grey) and the two other species
Barbatula barbatula
and
Barbatula quignardi
, for the two sizes classes (36 to 47 mm SL and> 47 mm SL).
only by a larger interorbital width (35.5 to 40.5% HL,
vs.
21.4 to 35.6% HL; Fig. 4B).
For general appearance, see figure 1; morphometric data are provided in table I. Medium sized species. The stone loaches belonging to
B. leoparda
present a marbled pigmentation on the body with brown-dark blotches on the belly and in the jugular area for specimens larger than
47 mm
SL (
Fig. 2
). The lateral profile is straight and the head is round- ed. The body is cylindrical in front of the dorsal-fin origin and the tail is laterally compressed. The body width is equal on all its length, except for the head. The anterior and posteri- or nostrils are close to each other without any space between them. The mouth bears 3 pairs of barbels, and the last one located at the corners does not reach the posterior part of the eye. The upper lip is slim with a medial incision not exceeding the half of the width of the upper lip (
Fig. 3
). The mental lobes on the lower lip are not well marked (
Fig. 3
). This species has III simple and 7 to 8 branched dorsal fin rays (
holotype
III7), III simple and 5 to 6 branched anal fin rays (
holotype
III5), I simple and 9 to 11 branched pectoral fin rays (
holotype
I11), I simple and 6 to 7 branched pelvic fin rays (
holotype
I7), and 14 to 18 branched caudal-fin rays (
holotype
18). The lateral line is complete. Specimens larger than
47 mm
SL have a proportionally deeper caudal peduncle and a smaller dorsal fin than smaller specimens. Largest known specimen
62.6 mm
SL.