Microgaster godzilla (Hymenoptera, Braconidae, Microgastrinae), an unusual new species from Japan which dives underwater to parasitize its caterpillar host (Lepidoptera, Crambidae, Acentropinae) Author Fernandez-Triana, Jose https://orcid.org/0000-0003-0425-0309 Canadian National Collection of insects, 960 Carling Avenue, Ottawa, Ontario, Canada cnc.braconidae@gmail.com Author Kamino, Tetsuyuki Environmental Entomology and Zoology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University; 1 - 1, Gakuen-cho, Sakai, Osaka 599 - 8531, Japan Author Maeto, Kaoru Graduate School of Agricultural Science, Kobe University, 1 - 1 Rokkodai, Nada, Kobe 657 - 8501, Japan Author Yoshiyasu, Yutaka Environmental Entomology and Zoology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University; 1 - 1, Gakuen-cho, Sakai, Osaka 599 - 8531, Japan Author Hirai, Norio Environmental Entomology and Zoology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University; 1 - 1, Gakuen-cho, Sakai, Osaka 599 - 8531, Japan text Journal of Hymenoptera Research 2020 2020-10-30 79 15 26 http://dx.doi.org/10.3897/jhr.79.56162 journal article http://dx.doi.org/10.3897/jhr.79.56162 1314-2607-79-15 3332E63BE38E4E62BE938B040BD10E20 F84D10CA9CFB570884209A6E4D1BB835 4255353 Microgaster godzilla , Fernandez-Triana & Kamino sp. nov. Figs 1 , 2 Material examined. Holotype . JAPAN • ♀ , OPU; Honshu , Osaka Prefecture , Sakai City , Shoubu-ike Pond , 1.v.2017 , ex. Elophila turbata on 13.v.2017 , T. Kamino , voucher code: CNC924596 . Paratypes . JAPAN • 1 ♀ , 1 ♂ , CNC; Honshu , Osaka Prefecture , Moriguchi City , Yagumo-Higashimachi , 26.i.2017 , T. Kamino, voucher codes: CNC924597, CNC924599, 1 ♂ OPU; same locality and date than holotype , voucher code: CNC924598 ; 1♀ , OPU; Osaka Prefecture , Moriguchi City , Niwakubo , 30.ix.2016 ; 1♀ , 1♂ , OPU; Kyoto Prefecture , Kyoto City , Shimogamo , 15.ix.2017 . Diagnosis. Among all described species of Microgaster this species can be distinguished because of its unique combination of morphological characters. Color patterns are distinctive, especially having all legs almost entirely yellow (only apical 0.1 of metafemur, apical 0.3 of metatibia and metatarsus are dark brown to black), tegula dark brown, pterostigma brown, and metasoma dorsally with T1-T2 black and T3+ orange-yellow. Very few Microgaster species have all coxae yellow, and then the color of the tegula and/or metasoma dorsally is usually different. Beyond color, the combination of flagellomeres with relatively distinct setae (bristly), eyes convergent ventrally, face dull due to transverse, rugose sculpture (including indication of vermiculate rugosities towards sides), notauli barely marked by impression or sculpture (and overall sculpture of anteromesoscutum with fine and relatively shallow punctures), scutellar disc mostly smooth, mesopleuron without strong crenulated sulcus, T2 and T3 about same length, T3+ smooth, all tarsi with last segment enlarged, and large but simple tarsal claws are also of diagnostic value. Microgaster godzilla shares some features with the described species of Hygroplitis , most of which also have light-coloured legs, including all coxae in many species, large tarsal claws, and last segments of tarsi enlarged. However, most Hygroplitis species have the body depressed, notauli are strongly marked, antennae have three rows of placodes and the mesopleuron has a strong, crenulated sulcus. Although there is no available key that covers all described Hygroplitis and Microgaster , we found that all Palearctic, Nearctic and Oriental species previously described in those two genera differ from the diagnosis provided above for M. godzilla by at least one (usually more) characters. To facilitate future work on the genus we provide one-to-one comparisons of M. godzilla with every other previously described species of those two genera in the biogeographical regions relevant to the new species (see Suppl. material 5 for detailed comparisons). Description. Color . Head and mesosoma black; metasoma dorsally with T1-T2 black, T3+ orange-yellow; metasoma ventrally entirely orange-yellow, including hypopygium; antenna light brown; palpi orange-yellow; tegula and humeral complex dark brown; all legs almost entirely yellow (only apical 0.1 of metafemur, apical 0.3 of metatibia and metatarsus are dark brown to black); metatibial spurs white; pterostigma and most veins brown. Head . Eyes convergent ventrally; face dull due to transverse, rugose sculpture (including indication of vermiculate rugosities towards sides); frons with transverse striation; gena mostly smooth; space between ocelli with weak sculpture (a short but vague carina is barely defined); flagellomeres bristly, with relatively distinctive setae. Mesosoma . Overall sculpture of anteromesoscutum with fine and relatively shallow punctures; notauli barely marked by impression or sculpture; scutoscutellar sulcus deep and broad, with strong crenulae; scutellar disc mostly smooth, with very shallow and sparse punctures; propodeum entirely sculptured with coarse reticulation and a median, longitudinal carina; mesopleuron mostly smooth and shiny on dorsal third, mostly sculptured on ventral 0.6, without strong, crenulated sulcus; metapleuron coarsely sculptured. Metasoma . T1 and T2 strongly sculptured; T3+ smooth; T1 very broad, its width at posterior margin twice that at anterior margin, its median length 0.9 its width at posterior margin; T2 rectangular and about same length as T3; hypopygium mostly inflexible, with a weakly defined and small fold apico-ventrally; ovipositor sheaths with relatively long setae the setose part roughly half the length of metatibia. Legs . All legs with simple and large tarsal claws; all tarsi with last segment enlarged. Wings . Fore wing with relatively large and quadrangular areolet, vein R1 longer than pterostigma length, vein r longer than vein 2RS and much longer than areolet height, vein r arising beyond mid point of pterostigma; hind wing with vannal lobe entirely setose. Body measurements (in mm). Body length: 3.3; fore wing length: 3.2; ovipositor sheath length 1.28; metatibia length 0.62; metafemur length/width: 0.99/0.28; flagellomere 2 length/width: 0.26/0.07; flagellomeres 13+ missing in the two female specimens (holotype and one paratype) available to JFT for study and species description. Figure 1. Microgaster godzilla , female holotype. A habitus dorsal B habitus lateral C wings D head, frontal E details of antenna F head and mesosoma, lateral G head and mesosoma, dorsal. Distribution. The species has only been collected in the prefectures of Osaka and Kyoto in Honshu, Japan. Biology. Microgaster godzilla sp. nov. has been reared from Elophila turbata (Butler, 1881) ( Lepidoptera : Crambidae , Acentropinae ), whose larvae are aquatic. Each larva constructs a portable case from fragments of aquatic plants (e.g. Azolla spp., Trapa spp. and Spirodela spp.) and lives inside the case which is found slightly above or slightly under the water surface ( Yoshiyasu 1985 ). Females of M. godzilla walk over the floating plants while searching for hosts. Once the wasp finds a case, it repeatedly probes it with its antennae and moves around, eventually forcing the larvae to come out of the case, when it is parasitized by the wasp by quickly inserting its ovipositor. In some cases, M. godzilla completely dives underwater for several seconds, in order to search for the submerged caterpillar and force it out of the case. In all cases we observed, oviposition occurred above water, where the host larvae went trying to escape the wasp (Suppl. material 1-3). The wasp can also pierce through the case for oviposition and this behavior is almost equally frequent. Molecular data. Four DNA barcodes were obtained (two of them almost full length at 631 bp, the other two specimens rendered partial barcodes of 421 bp each). Those sequences correspond to BINBOLD:ADO8283 and are unique among all available sequences of Microgastrinae in BOLD, with the closest BIN (an undescribed Microgaster species from Papua New Guinea) differing by 34 bp (5.2%) (Suppl. material 4). Etymology. The species is named after Godzilla (Japanese: ゴジラ , Hepburn: Gōjira ), a fictional monster (kaiju) that became an icon after the 1954 Japanese film of the same name and many films afterwards. The wasp name is intended to respectfully honour one of the most recognizable symbols of Japanese popular culture worldwide. The wasp's parasitization behaviour bears some loose resemblance to the kaiju character, in the sense that the wasp (after diving underwater to search for its host, a moth caterpillar) suddenly emerges from the water (to parasitize the host), similar to how Godzilla suddenly emerges from the water in the movies. Additionally, Godzilla has sometimes been associated, albeit in different ways, with Mothra (Japanese: モスラ , Hepburn: Mosura) another kaiju that is typically portrayed as a larva (caterpillar) or adult moth. The generic boundaries between Microgaster and Hygroplitis There is some evidence that Hygroplitis may just be a species-group of Microgaster ( Fernandez-Triana et al. 2020 ). Data based on DNA barcodes strongly suggest so (e.g., Suppl. material 4; see also Smith et al. 2013 ). Hygroplitis could represent a derived group, specialized in parasitizing aquatic or semiaquatic microlepidoptera; in fact one of the two previously known aquatic microgastrine species belongs to Hygroplitis (Morley, 1936). The presence in this genus of simple tarsal claws, which are elongate and strongly curved (like in Fig. 2D-F ), has been suggested to be one of the main characteristics that allows hymenopterans to grip the substrate when entering the water looking for hosts ( Bennett 2008 ). Figure 2. Microgaster godzilla , female holotype. A details of scutellar complex and propodeum B metasoma, dorsal C metapleuron and metasoma, lateral D details of ocelli dorsal E tarsal claw F hypopygium and ovipositor. Microgaster godzilla sp. nov. could be considered to be intermediate between those two genera but is not the first Microgaster reported to be like that. Another species, Microgaster deceptor Nixon, 1968, has similar simple tarsal claws which are relatively large, and it was also considered as intermediate between Hygroplitis and Microgaster by Nixon (1968 : 55), who treated Hygroplitis as only a species group of Microgaster . Solving the generic boundaries between these genera, or perhaps synonymizing Hygroplitis under Microgaster , is beyond the scope of this paper; in any case, for the time being we prefer to place the new species within Microgaster .