Two new species of amphibian nematodes from Bhadra Wildlife Sanctuary, Western Ghats, India.
Author
Rizvi, Anjum Nasreen
text
Zootaxa
2009
2013
58
68
journal article
10.5281/zenodo.185867
e648f5e0-fb90-40bd-af63-cf0493ab6140
1175-5326
185867
Narsingiella clinotarsi
sp. nov.
(
Figures 3
&
4
; Table 3)
General Morphology
: Body cylindrical rounded, tapering towards extremities. Mouth surrounded by three lips, bearing two pairs of submedian papillae and a pair of lateral amphids. Lips are also provided with digitiform projections (
Fig. 4
A). Cervical cordons, broad with minute spines at the outer border (
Figs. 3
A & 4 B) Stoma, rectangular, 18– 20 µm. Head distinct, not continuous with the body contour. Pharynx rectangular with esophageal corpus, cylindrical, small pyriform non-valvated bulb opening into a terminal valvated bulb. Excretory pore anterior to bulbs.
TABLE 3
. Measurements (in mm) of
Narsingiella clinotarsi
sp.nov
and
N. narsingi
Rao.
(N = Total number of specimens; ABD= Anal Body Diameter, a = L/W; b=L/E; c = L/T; V= Vulva /L x100)
Parameters
Narsingiella clinotarsi
sp. nov.
Narsingiella narsingi
Rao
| Male Holotype |
Female Paratype |
Male |
Female |
| N 1 |
1 |
15 |
20 |
| Length(L) 3.54 |
4.04 |
3.15–5.38 |
4.14–6.56 |
| Width(W) 0.26 |
0.40 |
0.22–0.28 |
0.25–0.40 |
| Esophagus(E) 0.96 |
1.10 |
0.65–0.85 |
0.83–1.00 |
| Anterior Bulb 0.075 |
0.085 |
not stated |
not stated |
| Posterior Bulb 0.10 |
0.10 |
not stated |
not stated |
| Excretory pore 0.50 |
0.55 |
0.34–0.47 |
0.45–0.60 |
| Stoma 0.018 |
0.020 |
not stated |
not stated |
| Tail (T) 0.18 |
0.24 |
0.16–0.25 |
0.30–0.36 |
| Anus 0.33 |
0.38 |
not stated |
not stated |
| ABD 0.12 |
0.17 |
not stated |
not stated |
| Vulva - |
2.0 |
- |
2.4–4.25 |
| Spicule 0.36; 0.38 |
- |
0.30–0.52 |
- |
| Gubernaculum 0.040 |
- |
0.05–0.08 |
- |
| Caudal Papillae 6:1:7 = 14 pairs |
- |
6:0:6=12 pairs |
- |
| a 13.6 |
10.1 |
not stated |
not stated |
| b 3.68 |
3.60 |
not stated |
not stated |
| c 19.6 |
16.8 |
not stated |
not stated |
| V - |
54.4 |
not stated |
35–42 |
Female
: Gonad didelphic, amphidelphic. Vulva situated at midbody with indistinct vulval lips. Uterus filled with eggs in different developing stages. Tail, small, conical with pointed tip.
Male
: Prominent preanal sucker almost close to anus present. Caudal papillae 6:1:7 = 14 pairs, 3 pairs of preanal papillae are pedunculated and 3 pairs are sessile and grouped together. One pair of sessile adanal papillae present. Out of the 7 pairs of postanal papillae, 6 pairs are grouped together and pedunculated, whereas one pair is sessile and situated at a distance of 45 μm. Caudal alae indistinct. Spicules two and unequal, shaft of spicule trilobed. Gubernaculum small. Tail with a fine tip.
Host:
Bicoloured frog,
Clinotarsus curtipes
(Jerdon)
-Deposited in Zoological Survey of
India
, Western Ghats Field Research Station,
Calicut
, Kerala,
India
, vide registration No. ZSI/WGFRS/V/A-677.
FIGURE 3.
Photomicrographs of Female
Narsingiella clinotarsi
sp. nov.
A. Head end showing stoma (Scale bar = 10 µm).B. Head end showing cervical cordons with minute spines (Scale bar = 10 µm). C. Posterior end (Scale bar = 100 µm). D. Esophageal bulbs and intestine (Scale bar = 100µm).E. Eggs in uterus (Scale bar = 50 µm). F. Tail end (Scale bar = 50 µm).
FIGURE 4.
Photomicrographs of Male
Narsingiella clinotarsi
sp. nov.
A. Head end showing lips with digitiform projections (Scale bar = 10 µm).B. Head end showing cervical cordons with minute spines (Scale bar = 10 µm). C. Posterior end (Scale bar = 50 µm). D. Arrangement of postanal papillae (Scale bar = 10 µm). E. Preanal sucker, gubernaculum and rectum (Scale bar = 50 µm). F. Postanal pedunculated papillae (Scale bar = 10µm).G. Preanal simple papillae (Scale bar = 10 µm). H Esophageal bulbs (Scale bar = 50 µm). I. Spicule with trilobed shaft (Scale bar = 20 µm).
Locality:
Haligekerae, Muthodi Range, Bhadra Wildlife Sanctuary, Karnataka,
India
. Lat. 13 0 29’ – N; Long. 75 0 36’ – E. Altitude:
2900 ft
. above MSL (approximately).
Site of infection:
Gut.
Type
material
:
Holotype
male: ZSI/
NRS
/IV/N/381,
Paratype
female: ZSI/
NRS
/IV/N/382 (Zoological Survey of
India
, Northern Regional Station, Dehradun,
India
.).
Diagnosis and relationships.
Narsingiella clinotarsi
sp. nov.
is characterized by male having long esophagus, two unequal spicules with trilobed shaft, small gubernaculum and 14 pairs of caudal papillae in the pattern as 6:1:7 and female having long esophagus, short conical tail and vulva situated at midbody. Both the sexes with lips bearing digitiform projections.
The new species differs from the only known species,
N. narsingi
Rao
in several important characters.
Esophagus in both sexes 1/3rd of body in
Narsingiella clinotarsi
sp. nov.
and 1/8–1/6th in
N.narsingi
,
lips of both sexes with digitiform projections in
N. clinotarsi
sp.nov.
, while lips of
N.narsingi
with three pairs of onchia.
Tail of female
N
.
clinotarsi
sp.nov.
is short and conical,while tail of
N. narsingi
female is long with tapering pointed end; Vulva situated at 50% of body length in
N
.
clinotarsi
sp.nov
as opposed to 35–42% in
N. narsingi
Male of
N
.
clinotarsi
sp. nov.
possess two unequal spicules with a trilobed spicule shaft (
Figure
4
I), whereas, spicules of
N. narsingi
are equal and lack trilobed shaft. Gubernaculum is short in
N
.
clinotarsi
sp.nov
(40 µm) and long in
N. narsingi
(50–80 µm). Number and pattern of caudal papillae in these two species also differs: 6:1:7 pairs in
N
.
clinotarsi
sp.nov
and 6:0:6 pairs in
N. narsingi
. All these characters differentiate the present specimens from the only known species of the genus
N. narsingi
and justifies its new species status.
Bicoloured frog,
Clinotarsus curtipes
(Jerdon)
is a new host record for the genus
Narsingiella
.