New or little-known Boreoheptagyia (Diptera, Chironomidae) in China inferred from morphology and DNA barcodes Author Lin, Xiao-Long https://orcid.org/0000-0001-6544-6204 College of Life Sciences, Nankai University, Tianjin, 300071, China Author Yu, Hai-Jun https://orcid.org/0000-0002-2460-3805 Center of Animal husbandry and Fisheries, Bijiang Agriculture and Rural affairs Bureau, Tongren, Guizhou, 554300, China Author Wang, Xin-Hua College of Life Sciences, Nankai University, Tianjin, 300071, China Author Bu, Wen-Jun College of Life Sciences, Nankai University, Tianjin, 300071, China Author Yan, Chun-Cai Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, 300387, China Author Liu, Wen-Bin https://orcid.org/0000-0001-6916-0692 Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, Tianjin Normal University, Tianjin, 300387, China skyliuwenbin@163.com text ZooKeys 2021 2021-05-28 1040 187 200 http://dx.doi.org/10.3897/zookeys.1040.66527 journal article http://dx.doi.org/10.3897/zookeys.1040.66527 1313-2970-1040-187 F7D0867DFB4948CAB89EFC27B06474E1 DAAA46DB28C3544797D60D5DE169BDFB Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992) Figs 1 , 2 , 3 , 4 Diamesa kurobebrevis Sasa & Okazawa, 1992: 58. Toyamadiamesa kurobebrevis Sasa & Kikuchi, 1995: 205. Boreoheptagyia kurobebrevis Endo, 2002: 12; Makarchenko et al. 2008 : 8; Ashe and O'Connor 2009 : 265. Material examined. Male (NKU & BOLD sample ID: LGS63), 1 male , China , Guizhou Province , Qiandongnan Miao and Dong Autonomous Prefecture , Leishan , Leigongshan Natural Reserve , Fangxiang , Getoucun , 26.396014°N , 108.260933°E , 1070 m a.s.l. , Malaise trap , 10-30. i. 2020 , leg. H.-J. Yu. 1 female , same as above . Diagnostic characters. B. kurobebrevis can be distinguished from other related species in having: antenna with five flagellomeres; wing membrane covered with microtrichia on entire surface. Costal extension 175 μm long. R with 31 setae, R1 with 34 setae, R4+5 with 31 setae. Superior volsella rounded; inferior volsella finger-like and well-sclerotized; gonostylus with 2 small megasetae. Adult male ( N = 1). Total length 2.95 mm. Wing length 2.60 mm. Total length/wing length 1.13. Wing length/length of profemur 2.17. Coloration (Fig. 1 ). Head, thorax and abdomen mostly dark brown. Ground color of scutum yellow, stripes and postnotum dark brown, scutellum brown, abdominal tergites almost uniformly yellow, hypopygium dark brown, femur yellow in basal three-quarter with brown ring apically, tibia pale medially with brown rings in basal one-fifth and distal one-third. Figure 1. Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992), male adult in ethanol. Head . Antenna with five flagellomeres. AR 0.82. Temporal setae 8, not separable into inner and outer verticals. Clypeus with 20 setae. Tentorium 75 µm long; 25 µm wide. Lengths of palpomere 1-5 (in µm ): 38, 50, 105, 158, 250. Length ratio of palpomeres 5/3: 2.38. Thorax (Fig. 2B ). Antepronotum with 9 anterolateral setae; acrostichals 28; dorsocentrals 17 in two rows; prealars 10. Scutellum with 46 setae. Figure 2. Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992), male adult A wing B thorax C hypopygium. Scale bars: 200 µm . Wing (Fig. 2A ). Brachiolum with 5 setae; wing membrane with macrotrichia on entire surface; costal extension 175 µm long. Distribution of setae on veins: R, 31; R1, 34; R4+5 3. Anal lobe well-developed; squama with 16 setae. VR 1.08. Legs . Length (in µm ) of spurs of: P1, 40; P2, 55 and 55; P3, 80 and 50. Width (in µm ) of tibial apex of: P1, 60; P2, 70 µm ; P3, 100. Comb on hind tibia with Comb of hind tibia with 12 setae. Lengths (in µm ) and proportions of legs as in Table 2 . Table 2. Lengths (in µm ) and proportions of legs of Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992) in China, male ( N = 1).

-	fe	ti	ta1	ta2	ta3	ta4	ta5	LR	BV	SV	BR
P1	1200	1380	920	500	270	80	130	0.67	3.57	2.80	2.64
P2	1300	1220	750	480	240	70	120	0.61	3.59	3.36	3.50
P3	1260	1450	900	500	250	70	90	0.62	3.97	3.01	3.89

Hypopygium (Fig. 2C ). Tergites IX without anal point. Tergites IX with 30 setae. Gonocoxite 300 µm long. Superior volsella rounded, 60 µm long. Inferior volsella well-sclerotized, finger-like, bearing 17 setae. Gonostylus 140 µm long, with two small megasetae. HR 2.14; HV 2.11. Genitalia of female ( N = 1) (Figs 3 , 4 ). Gonocoxite IX 100 µm long, 45 µm wide, with 7 setae. Cercus 88 μm long, 75 μm wide, with 47 setae and covered with macrotrichia. Seminal capsule 158 μm long, 75 μm wide, sclerotized part 98 μm long. Figure 3. Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992), female adult. Figure 4. Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992), female adult A genitalia with gonapophysis VIII and seminal capsules, ventral B cercus. Scale bars: 50 µm .