Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species Author Guglya, Yuliia text Zootaxa 2021 2021-08-03 5014 1 1 158 http://dx.doi.org/10.11646/zootaxa.5014.1.1 journal article 10.11646/zootaxa.5014.1.1 1175-5326 5158589 63EEF5A6-EAE0-438F-87BC-AF5806BD3641 Amauromyza (Amauromyza) carlinae (Hering) ( Figs. 45–48 , 349–354 ) Material examined: Ukraine : Poltava Region : near Luchky , 48°57’N , 34°09’E , 14.vii.2016 , Yu. Guglya , ex Echinops sphaerocephalus ( 1♂ ) . Kharkiv Region : near Petrivske , 49°10’N , 36°58’E , 14–15.vii.2020 , Yu. Guglya , ex Echinops sphaerocephalus ( 2♂ 3♀ ) . Hosts. Asteraceae : Carlina L., Cirsium Mill. ( Benavent-Corai et al . 2005 ) . Echinops sphaerocephalus L.—a newly recorded host plant. Mine. ( Fig. 45 ) Several (up to eight) larvae together form one large whitish-brown blotch mine. Pupation takes place outside the mine. Puparium. ( Figs. 46–48 ) Reddish-brown, glossy, 2.5 mm long, with deep segmentation; surface quite smooth except for wide black spine bands. Posterior spiracles set on stout conical protuberances entirely strongly separate; orange, with five hook-like bulbs set in a circular configuration: three (smaller) located dorsally and two (larger) ventrally. Anal plate distinctly protruding above the surface of the puparium viewed from the side and directed ventrally. Cephalopharyngeal skeleton. ( Fig. 349 ) Right mouthhook much larger than the left, both with ventro-anterior portion distinctly abducted. The right mouthhook bears two sharp accessory teeth and the left hook bears one tooth. Parastomal bar short, curved dorso-anteriorly. Intermediate sclerite wide and short, 0.8× as long as maximum height of left mouthhook. The mouthhook, intermediate sclerite and dorso-anterior portion of the pharyngeal sclerite are strongly sclerotized, posterior half of the dorsal cornu and the ventral cornu much less so. The ventral cornu bears a small “closed” window. Indentation index 83. Female head. ( Figs. 350, 351 ) Brown, with antenna and face black; orbit not projecting above eye in profile; 2 orb s, 4 fr s; lunule low, narrow reaching the level of the anterior fr s; pped small, rounded; gena medially 0.15× as high as maximum height of eye. Female genitalia. ( Figs. 352–354 ) Capsule of spermatheca relatively small, 0.15× as high as height of anterior part of oviscape. Proctiger relatively wide, 2.62× as long as maximum width; cylindrical in posterior half narrowing dramatically and bearing an X-shaped well sclerotized appendage anteriorly. Surface of the posterior half covered with numerous fine spines. Posterior margin of proctiger flattened; cercus relatively wide and acute apically. Spermathecae unequal in size, dark brown, torus-shaped, without basal collar. Capsule of spermatheca 0.47× as high as maximum width. Spermathecal duct weakly sclerotized. Distribution. Spain , France , Germany , Poland , Slovakia ( Papp & Černý 2016 ). Ukraine (first record).