A taxonomic revision of genus Labrundinia Fittkau, 1962 (Diptera: Chironomidae: Tanypodinae) Author Silva, Fabio Laurindo Da Author Fonseca-Gessner, Alaide Aparecida Author Ekrem, Torbjørn text Zootaxa 2014 3769 1 1 185 journal article 46323 10.11646/zootaxa.3769.1.1 ceef5966-d5f4-4683-847c-7fbd85661e59 1175-5326 250728 705567C6-C912-4A19-9DE2-726254E17076 Labrundinia neopilosella Beck and Beck, 1966 ( Figs 42–44 ) Labrundinia neopilosella Beck and Beck, 1966 : 341 (description of adults and immatures); Roback 1971 : 278 (description of male); Roback 1987: 193 (description of immatures). Material examined. Type material: Holotype male with larval exuviae, USA : Florida , Flagler County, Rayonier Ditch, 3.vi.1964 , W. Beck ( USNM 69125). Paratype : 1 male with pupal and larval exuviae same data as holotype except for 27.ii.1965 . Additional material: 3 larvae , USA : North Carolina , Forsyth, Belews Lake, 16.ix.1985 , W. Beck. 1 male with pupal exuviae, South Carolina , Pickens, Clemson University, 16.ix.1985 , P. L. Hudson; 1 larva as previous except for Anderson, Fish Pond, 25.iii.1977 , P. H. Carlson and T. R. White. Diagnostic characters. Labrundinia neopilosella differs from other Labrundinia species by the combination of the following Characters. Adult male : abdominal tergite I pale brown, T II–IV with continuous brown transverse band near proximal margin, T V–VIII completely brown; hypopygium pale brown, sternapodeme with reduced anterior process. Pupa : thoracic horn ovoid; preapical indentation absent; thoracic horn about 2.5–2.9 times as long as wide. Larva : Surface of head capsule smooth, lateroventral spine group present with several conspicuous spines; subbasal seta of posterior parapod serrated; bifid claw with U-shaped lower indentation. Description. Adult male (n = 4 unless otherwise stated) Size. Total length 1.6–2.0 (3) mm. Wing length 1.0– 1.2 mm . Total length/wing length 1.33–1.75 (3). Wing length/ profemur length 2.80–2.85 (3). Coloration . Head pale brown with dark brown occipital margin; pedicel and antenna brown; maxillary palp pale brown. Thorax brown with dark scutum; antepronotum pale brown; supraalar callus pale brown. Wing membrane transparent without any marking. Legs pale. Femur I–III pale. Tibia I–II pale, Ti III pale brown. Tarsomere 1–5 pale to pale brown. Abdominal tergite I pale brown, II–IV with continuous brown transverse band near proximal margin, T V–VIII completely brown; hypopygium pale brown ( Fig. 42L ). Head ( Figs 42A–E ). Temporal setae 10–14 (3), uniserial ( Fig. 42B ). Eye ratio 0.84–0.96 (2). Tentorium ( Fig. 42C ) 98–128 (2) Μm long. Clypeus 58–92 (2) Μm long, 53–63 (2) Μm wide at largest part, bearing 12–17 setae. Cibarial pump 144–165 (3) Μm long ( Fig. 42D ), with anterior margin concave. Lengths of palpomeres 1–4 (in Μm): 23–29 (3); 41–47 (3); 88–92 (3); 90–120 (2); palpomeres 5 broken off. Antennal flagellum 582–678 (3) Μm long, diameter of pedicel 96–108 Μm, apical setae single ( Fig. 42E ), AR 1.15–1.19 (3). Thorax . Antepronotum with 3–5 (2) lateral setae. Acrostichals 30–36 (2), biserial, diverging evenly posteriorly, starting close to antepronotum and almost reaching scutellum; dorsocentrals 18–26, irregularly uniserial; prealars 7–10; supraalars 3. Anapleural suture ratio 0.35–0.50 (2). Scutellum with transverse row of 8–10 (3) setae and group of numerous fine anterior setae. Wing ( Fig. 42F ). Width 0.3 mm . Costa 0.8–1.0 mm long. R2+3 absent. VR 0.64–0.71. WW 0.26–0.30. Brachiolum with 2 setae. Squama setiferous. Legs ( Figs 42G–K ). Fore leg: width at apex of tibia 32–39 Μm ( Fig. 42G ), tibia with single, apical and pectinate spur 15–16 (3) Μm long ( Fig. 42H ), with three lateral teeth and two preapical setae; ta1-4 without any preapical pseudospurs. Mid leg: width at apex of tibia 31–39 Μm long ( Fig. 42I ), tibia with single, apical and pectinate spur 17–18 (3) Μm long with three lateral teeth ( Fig. 42J ) and two preapical setae; ta1-4 each with two preapical pseudospurs. Hind leg: width at apex of tibia 29–39 Μm long ( Fig. 42K ), tibia without spur; comb with 5 (2) bristles; ta1-4 without any preapical pseudospurs. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 27 . Hypopygium ( Fig. 42M ). Tergite IX arched, with 10–14 posterior setae. Membranous anal point rounded, notched apically. Phallapodeme 39–49 (3) Μm long. Sternapodeme with reduced anterior process. Gonocoxite cylindrical, 84–96 Μm long, 41–46 Μm wide, with slightly concave inner margin. GcR 1.86–2.23. Gonostylus simple and slender, 58–63 Μm long; megaseta 9–12 (3) Μm long. HR 1.36–1.53. HV 2.64–3.10 (3). Adult female (n = 1) Size. Total length 0.8 mm . Wing length 0.9 mm . Total length/wing length 0.98. Wing length/ profemur length 2.68. Coloration . Head, pedicel, antenna and maxillary palp pale brown. Thorax pale brown with dark scutum. Wing membrane transparent without any marking. Legs pale. Abdomen completely pale brown, not very discernible. Seminal capsules brown. TABLE 27. Lengths (in µm) and proportions of leg segments in Labrundinia neopilosella Beck and Beck , adult male (n = 2–3 unless otherwise stated). fe ti ta1 ta2 ta3 p1 414–453 452–484(1) 254–274 207–218 133–145 p2 487–523 352–416 453–507 180–242 133–141 p3 469–477 484–586 460–500 219–242 148–179 ta4 ta5 LR BV SV p1 103–109 62–63 0.60–0.63 2.26–2.38 2.95–3.34 p2 101–102 63–70 1.30–1.38 2.68–2.69 1.68–1.74 p3 101–109 63–78 0.95–098 2.45–2.48 1.86–1.95 Head . Temporal setae 10, uniserial. Eyes ratio 1.04. Tentorium 107 Μm long. Clypeus 74 Μm long, 71 Μm wide at largest part, bearing 19 setae. Cibarial pump 142 Μm long, as in male. Lengths of palpomeres 1–5 lengths (in Μm): 34; 52; 65; 92; 147. Antennal flagellum 299 Μm long, diameter of pedicel 49 Μm, AR 0.42. Thorax . Antepronotum with 6 lateral setae. Acrostichals 36, irregularly biserial, starting close to antepronotum; dorsocentrals 32, irregularly uniserial; prealars 11; supraalars 3. Scutellum with transverse row of 9 setae and group of numerous fine anterior setae. Wing . Width 0.3 mm . Costa 0.7 mm long. R2+3 absent. VR 0.82. WW 0.35. Brachiolum with 2 setae. Squama setiferous. Legs . Fore leg: width at apex of tibia 36 Μm, tibia with single, apical and pectinate spur 14 Μm long, with three lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Mid leg: width at apex of tibia 28 Μm, tibia with single, apical and pectinate spur 18 Μm long, with three lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Hind leg: width at apex of tibia 27 Μm, tibia without spur; comb setae not observed; preapical pseudospurs on ta1-4 not observed. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 28 . TABLE 28. Lengths (in µm) and proportions of leg segments in Labrundinia neopilosella Beck and Beck , adult female (n = 1). fe ti ta1 ta2 ta3 p1 334 313 242 141 107 p2 470 361 – – – p3 417 484 441 241 100 ta4 ta5 LR BV SV p1 85 62 0.77 2.25 2.67 p2 – – – – – p3 99 77 0. 91 2.59 2.04 Genitalia. Gonapophysis VIII broadly rounded, 67 Μm long. Coxosternapodeme 108 Μm long. Postgenital plate rounded. Cerci oval-quadrate, 38 Μm long and 21 Μm wide; with 4 elongated setae. Labia with inconspicuous microtrichia. Notum length 108 Μm long. Seminal capsules oval, 50 Μm long, 30 Μm wide, with conical shaped necks. Length ratio SCa/No 0.47. Pupa (n = 4 unless otherwise stated) Size. Abdomen1.7–1.8 (3) mm long in male, 1.8 (1) mm long in female. Coloration . Exuviae mostly pale brown; thoracic horn and apex of anal lobe brown. FIGURES 42A–M. Labrundinia neopilosella Beck and Beck , adult male. A. Head, frontal view. B. Temporal setae. C. Tentorium. D. Cibarial pump. E. Apex of antenna. F. Wing. G. Apex of fore tibia. H. Fore tibial spur. I. Apex of mid tibia. J. Mid tibial spur. K. Apex of hind tibia with comb. L. Abdominal coloration pattern, dorsal aspect. M. Hypopygium, left: ventral aspect, right: dorsal aspect. FIGURES 43A–E. Labrundinia neopilosella Beck and Beck , pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect. FIGURES 44A–I. Labrundinia neopilosella Beck and Beck , larva. A. Head with chaetotaxy and detail of lateroventral spine group, left: ventral aspect, right: dorsal aspect. B. Antenna. C. Apex of antenna. D. Maxillary palp. E. Mandible. F. Ligula and paraligula. G. Pecten hypopharyngis. H. Subbasal seta of posterior parapod. I. Bifid claw of posterior parapod. Cephalothorax ( Figs 43A–C ). Frontal apotome smooth ( Fig. 43A ). Wing sheath smooth 0.7–0.8 (3) mm long. Thoracic horn 219–231 Μm long and 76–101 Μm wide ( Fig. 43B ), THR 2.54–2.91, preapical indentation absent. Membranous preapical papilla 22–37 Μm long ( Fig. 43C ), PTH 0.10–0.17, aeropyle tube simple, elongated, 28–29 (2) Μm long; plastron plate small. Horn sac well developed, completely filling respiratory atrium. Reticulation of respiratory atrium indistinct. Basal lobe wedge-shaped. Thoracic comb with 8–10 conical tubercles ( Fig. 43B ). Abdomen ( Figs 43D–E ). Tergite I with scar 114–120 (2) Μm long. T I–VI without shagreen, T VII–VIII with shagreen basally concentrated. Abdominal chaetotaxy as in figure 43D. Abdominal segment VII with 4 LS-setae. A VIII with 5 LS-setae. Anal lobe 234–258 (3) Μm long and 148–171 Μm wide ( Fig. 43E ), outer margins sclerotized, with 8 (2) spines, longest spine 14–16 (3) Μm long, inner margins of lobes membranous. ALR 1.58–1.64 (3). Male genital sac extending somewhat beyond apex of anal lobe. 4th instar larva (n = 8 unless otherwise stated) Coloration . Head pale yellow; postoccipital margin brown. Second antennal segment brown; distal tooth of mandible and apex of ligula brown. Abdomen pale yellow; procercus and anal setae pale brown. Posterior parapod claws all pale yellow. Head ( Fig. 44A ). Length 438–463 (4) Μm, 254–297 (4) Μm wide. IC 0.56–0.75. Surface smooth; lateroventral spine group present, well developed, with 7–10 (7) spines; posteroventral spine group absent. Ventral cephalic setae S9, S10 and SSm forming a more or less straight line diagonal to longitudinal axis of head capsule ( Fig. 44A ). Antenna ( Figs 44B–C ). Length 246–288 Μm, A1 148–189 Μm long, with ring organ located 0.77–0.86 from base, A2 68–78 Μm long. AR 1.85–2.13 (7). Blade longer than A2 over-reached by accessory blade. Maxilla ( Fig. 44D ). Basal palp segment 24–38 Μm long and 8–18 Μm wide at the middle, with ring organ 0.76– 0.91 (6) from base. PR 2.14–3.20 (3). APR 4.80–7.60. Mandible ( Fig. 44E ). Length 60–73 Μm. Sensillum campaniformium located 0.68–0.85 from apex, basal tooth bifid, with seta subdentalis projecting from sloping end towards apical tooth, accessory tooth present, AMD 2.28– 3.05. Mentum and M appendage . Dorsomental teeth reduced; pseudoradula uniformly granulate. Hypopharyngeal complex ( Figs 44F–G ). Ligula 52–65 Μm long, 24–27 Μm wide, with row of 5 teeth. IO 0.95– 0.99 (7), MO 1.08–1.15. Paraligula bifid, 20–29 Μm long, inner tooth 19–26 (7) Μm long, shorter than outer tooth. Pecten hypopharyngis with 8 teeth almost equal in size. Body ( Figs 44H–I ). Without lateral fringe. Anterior parapods with simple claws. Procercus 125–151 (6) Μm long, 22–40 Μm wide, with 7 anal setae 494–650 (5) Μm long. L/ W 4.60 –6.00 (5). Supraanal seta well developed. Anal tubules 173–210 (4) Μm long. Posterior parapod 251–251 (4) Μm long; subbasal seta on posterior parapod serrated, with 5–9 (7) spines basally ( Fig. 44H ); parapod apex with numerous simple claws; bifid claw with Ushaped lower indentation ( Fig. 44I ). B/C 0.88–0.96 (7). Remarks. Adult males of Labrundinia neopilosella appear most similar to those of L. setosa in the abdominal coloration. However, the completely brown abdominal tergite VI and the pale brown hypopygium present in L. neopilosella contrast the anteriorly brown abdominal tergite VI (anterior ¾) and the dark brown hypopygium in L. setosa . The pupae of L. neopilosella appear most similar to those of L. becki but can be distinguished from this species by the insertion of aeropyle tube into the upper margin of the preapical indentation in the thoracic horn. The larvae of L. neopilosella resemble those of L. becki and L. longipalpis , but may be separated from the former by the basally serrated subbasal seta and the bifid claw with U-shaped lower indentation of the posterior parapod. The subequal inner and outer teeth of the ligula and the pecten hypopharyngis with 7 teeth distinguish larvae of L. neopilosella from those of L. longipalpis . Labrundinia neopilosella is widespread in the United Stated (Roback 1987). The larvae were collected in in small lakes, creeks and unpolluted pools.