Taxonomic revision of Afrotropical Laccophilus Leach, 1815 (Coleoptera, Dytiscidae) Author Bistroem, Olof Author Nilsson, Anders N. Author Bergsten, Johannes text ZooKeys 2015 542 1 379 http://dx.doi.org/10.3897/zookeys.542.5975 journal article http://dx.doi.org/10.3897/zookeys.542.5975 1313-2970-542-1 026407877355425BAB10BF1674510F12 Taxon classification Animalia Coleoptera Dytiscidae Laccophilus alluaudi Regimbart , 1900 Figs 35-36, 233-234, 392, 531 Laccophilus alluaudi Regimbart 1900 : 373 (original description, faunistics); Zimmermann 1920a :16 (catalogue); Guignot 1955d : 67 (discussion); Guignot 1955f : 141 (faunistics, discussion); Guignot 1959a : 544, 548 (description, faunistics); Guignot 1959c : 76, 77, 78, 79 (discussion, faunistics); Guignot 1959e : 72 (faunistics); Guignot 1961a : 931 (faunistics); Bertrand and Legros 1971 : 244 (faunistics, biology); Wewalka 1980 : 724, 726 (faunistics, discussion); Rocchi 1991 : 79, 86 (faunistics); Nilsson 2001 : 240 (catalogue, faunistics); Pederzani and Rocchi 2009 : 95 (faunistics, list); Nilsson 2015 : 208 (catalogue, faunistics). Type locality. Madagascar: Diego Suarez. Type material, studied (5 exs). Lectotype (by present designation): male: "Madagascar Diego-Suarez Ch. Alluaud 1893 / male symbol / Cotype / Museum Paris col. Guignot" (MNHN). - Paralectotypes: Same data as in lectotype, but two of the specimens with female symbol (4 exx. MNHN; habitus in Fig. 392). Additional material studied (189 exs.): Madagascar: "Foret d'Ambre Lat -12.4754 Lon 49.2173 coll. Balke & Monaghan, BMNH(E)670568_MSL007: B10, 19.11.04 / Laccophilus alluaudi Regb . det. Bergsten" (1 ex. NHRS); same data but "BMNH(E)670572_MSL007: CO2" (1 ex. NHRS); "Antsiranana II Mt d'Ambre , Grande cascade stream parallel to GC in deep very steep gorge, mostly isolated pools 17.11. 2004, N: - 12,49920 E : 49,17600, 800 m Balke et al" (29 exs. NHRS, 4 exx. MZH); "Antsiranana II Foret d'Ambre , small water hole in dry streambed, gardenland at edge of dry forest 19.11. 2004 N-12,47540 , E49,21730 , 545m leg. Balke" (2 exs. NHRS); "Antsiranana 1 Mtd French Streampool 12.11. 2004 N-12,33360 , E49,35350 , 171 m, leg Balke et al" (12 exs. NHRS); "Antsiranana 1 Mt. d'Ambre 16.11. 2004, N-12,52830 , E49,17253 , 1020 m Balke et al." (1 ex. NHRS); "Toli, NW Ft Dauphin, forest, watersource, P54E: N: E:: m 19.5. 2006 Bergsten et al. / Laccophilus alluaudi det. Bergsten/BMNH(E): <?94192> DNA voucher / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Toli, NW Ft. Dauphin, creek with gravel, stones and sand in rainforest along the creek, in small water holes and ( Madaglymbus ) in waterpool on a large rock with wood and leaves 19.5. 2006 N-24°45.583, E46°51.821, 300 m Bergsten et al." (2 exs. NHRS); "Ambilobe, Anjiabe Ambony Antsabe: Galoko Mts, hygropetric cascade; alt. 50 m, P2506M N - 13.6093 E 48.7212 , 23.11. 2004 leg. Monaghan, Andriamparany, Balke / BMNH(E) <794160> DNA voucher / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Anjiabe Ambony, Ambilobe Antsabe stairways-like cascade with vertical steps, exposed, extremely hot day 23.11. 2004 N-13,60930 , E48,72120 , 303 m Balke et al." (17 exx. NHRS); "Ambilobe, Anjiabe Ambony Antsabe: waterhole in streambed on clearing: 50 m, P25MD11 N - 13.648 E 48.721 , 21.11. 2004, leg. Balke et al/BMNH(E) <794189> DNA voucher / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Toli, Taolanaro; Isaky Ivondro, Foret Manangotry, running water, P67B: N: -24.859: E: 46.862: 310 m, 9.4. 2007 leg. Ranarilalatiana et al / DNA voucher BMNH <830767> MSL399:H4 / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Ants, Sambava, Marojejy, Marojejy NP: Forest stream P57BI01: N: -14.437: E: 49.773: 464 m, 6.12. 2006 leg. Isambert et al. / DNA voucher BMNH <830690> MSL399:A11 / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Toam, Ambatondrazaka, Zahamena; Zahamena NP: Stream P60BI08 N: -17.505: E: 48.724: 1054 m, 30.12.2006 leg. Isambert et al. / DNA voucher BMNH <830696> MSL399:B5 / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); same data but "P60BI06 N: -17.508: E: 48.724: 1068 m" and "BMNH <830701> MSL399:B10" (1 ex. NHRS); same data but "Zahamena NP, Analamaintsoa 1st stream before Camp 1 within a few m from the GPS point P60BI06, 30.12. 2006 N-17,50617 , E48,72400 , 1068 m" (4 exs. NHRS); "TOAM Ambatondrazaka Zahamena NP on the way to camp 2 to Fenerive Est "Route des contrebandiers" P60BI29 02.1. 2007, N: - 17,54167 E : 48,72183, 1322m leg. Isambert et al" (37 exs. NHRS); same data but "Analamaintsoa, 2nd stream btw Camp 1 and 2, stream of pools 31.12. 2006 N-17,51850 , E48,72217 , 1075 m" (4 exs. NHRS); same data but "Analamaintsoa, 3rd stream before Camp 1, stream of pools, almost dry P60BI08, 30.12. 2006, N-17,50500 , E48,72450 , 1054 m Isambert et al" (10 exx. NHRS); "Montagne des Francais, Lat. -12.3336 Lon. 49.3535 leg. Balke & Otke, BMNH(E)_671210 MSL014; 1/1/1904 / DNA voucher BMNH <671210> MSL014: H02/ Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Saratanana, leg. Lees & Ranaivosolo MNH(E)_672835_MSL028; 1/1/1904 /DNA voucher BMNH <672835> MSL028: B05 / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Antsabe Lat -13.6093 Lon 49.7212, Balke leg. BMNH(E) _670700_MSL008 23.9. 2004/1904/DNA voucher BMNH <670700> MSL008: E10/ Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "Ants, Nosy Be: Lokobe R.N.1, 50 m, 2004/DNA voucher BMNH(E) <794171> / Laccophilus alluaudi det. Bergsten" (1 ex. NHRS); "R.N.I. Lokobe Nosy Be, 50 m, 15.12. 2004 Ravo" (1 ex. NHRS); "Mahajanga: Boeny Mahavavy Kinkony RS. S16.05648 , E045.76371 , 55 m.a.o., 5.12. 2009 water net, field, Bergsten et al. (19 exs. NHRS); same data, add: "000000465 NHRS-JLKB" (1 ex. NHRS); "Mahajanga: Melaky: Tsingy de Bemaraha NP, S19.03572 , E044.77507 , 66m m.a.o., 15.12. 2009, water net, field, Bergsten et al." (12 exs. NHRS); same data but " S18.75643 , E044.71398 , 119 m.a.o., 17.12. 2009" (2 exs. NHRS); same data but " S19.03419 , E044.77499 , 41 m.a.o., 15.12. 2009" (2 exs. NHRS); same data but " S18.75724 , E044.71239 , 72 m.a.o., 17.12. 2009" (3 exx. NHRS); same data, add: "000000468 NHRS-JLKB" (1 ex. NHRS); same data but " S19.14114 , E044.81245 , 45 m.a.o., 14.12. 2009/000000466 NHRS-JLKB" (1 ex.NHRS); "Prov. Antsirarana, P.N. Montagne d'Ambre , elev. 960 m 26-29.1. 2001/ N -12*30'52" E 49*10'53" leg. Irwin & al, malaise trap" (1 ex. CAS); "Prov. Antsirarana, P.N. Montagne d'Ambre , elev. 1125 m 30.5.-6.6. 2001/ N - 12°31'13" E 49°10'45" leg. Irwin & Hala, malaise trap" (1 ex. CAS); "Prov. Antsirarana, P.N. Montagne d'Ambre , elev. 1125 m 21-26.4. 2001/ N - 12°31'13" E 49°10'45" leg. Irwin & Hala, malaise trap" (1 ex. CAS); "Prov. Antsirarana, Sakalava beach, dwarf littoral forest 10 m, 13-20.8. 2001/ N - 12°15'46" E 49°23'51" Irwin & Hala leg., malaise trap - across sandy trail" (1 ex. CAS); "Andjamangirana (Majunga) 19.10. 2001/stream in dry forest, upstream. Rice field area (road to Tsaratanana) 200 m a.s.l., 30,8 °C, 0.008 mS/cm/Gerecke & Goldschmidt leg." (1 ex. BMNH, 1 ex. MZH); "Anjozorobe (Antananarivo) Ravoandrina Riv. Ampanakamonty 21.7.2001 / 1280 m asl, 12,8 °C, 0,078 mS/cm/Gerecke & Goldschmidt leg." (1 ex. BMNH); "SE-Mad. Res . Nat. Integr. de Andohahela (NW Ft. Dauphin) Parcelle 1 (versante E) - 300 m foresta pluviale 26.5. 1991/Bartolozzi, Taiti, Raharimina leg. / Laccophilus alluaudi Reg . det. Rocchi 1991 "(2 exs. CSR); "E-Mad. Ampamoho nr Andilamena 1200-1300 m asl, 18-20.1. 1995 Dunay & Janak" (1 ex. MZH); "Fiananrantsoa, Mania River S Ilaka, 900 m 27 rd km NNW Ambositra 23.10. 2001 Schuh leg." (1 ex. NMW); "Foret de Fito, ex. coll. Dr. Breuning" (1 ex. MRAC); "Prov. de Tamatave, Foret de Perinet 17.7. 1970 Pederzani / Laccophilus lateralis Sharp det. Pederzani" (1 ex. CSR; determination uncertain). Diagnosis. Laccophilus alluaudi forms together with Laccophilus comes , Laccophilus tigrinus , Laccophilus pseustes and Laccophilus furthi a distinct group of species characterized by quite similar colour pattern of body and male genitalia exhibiting same ground plan. Penis of Laccophilus alluaudi , Laccophilus comes and Laccophilus furthi is not evenly curved in lateral view as in Laccophilus tigrinus , but angled. Longitudinal markings of Laccophilus comes reach humeral region while in Laccophilus alluaudi and Laccophilus furthi corresponding markings fade away before reaching humeral region. Finally, penis (dorsal aspect) in Laccophilus alluaudi is strongly twisted, while almost straight in Laccophilus furthi . Furthermore Laccophilus comes penis is in lateral view medially slightly depressed and immediately after angle towards apex there is a minute, sharp knob which is lacking in Laccophilus alluaudi . Description. (See description of Laccophilus comes ; only diagnostically important differences noted): Body length 3.4-3.9 mm, width 1.9-2.1 mm. Dorsal, colour pattern (Fig. 392); slightly less pronounced in comparison with Laccophilus comes (Fig. 390). Elytra: Longitudinal markings brownish to ferrugineous; less pronounced in comparison with material of Laccophilus comes ; especially humeral region with reduced dark markings (Fig. 392). Ventral aspect: Apical ventrite as in Fig. 35. Male genitalia: Penis in lateral aspect from base region, almost straight to extreme apex, which is slightly bent upwards; in dorsal aspect, penis bent at right but less so than in Laccophilus comes (Figs 233-234). Female apical ventrite as in Fig. 34. Distribution . Madagascar (Fig. 531). Also reported from the Comoros (e.g. Guignot 1958 b). Collecting circumstances. Label data indicate that Laccophilus alluaudi occurs in both standing and running waters. Collected in a creek with gravel, stones and sand in rainforest along the creek, in small water holes and together with Madaglymbus in water pool on a large rock with wood and leaves.