Description of the puparium of Cyphomyia albitarsis (Diptera: Stratiomyidae: Clitellariinae) from Colombia Author Torres-Toro, Juliana Grupo de Entomología, Universidad de Antioquia, Medellín, Colombia & corresponding author: e-mail: julianaerobia @ gmail. com Author Wolff, Marta Grupo de Entomología, Universidad de Antioquia, Medellín, Colombia text Acta Entomologica Musei Nationalis Pragae 2019 Acta. Ent. Mus. Natl. Pragae 2019-12-27 59 2 555 560 http://dx.doi.org/10.2478/aemnp-2019-0048 journal article 10.2478/aemnp-2019-0048 1804-6487 5340551 1358645B-C7D9-4F1B-8088-5DC028D82D8A Cyphomyia albitarsis ( Fabricius, 1805 ) ( Figs 1–5 ) Stratiomys albitarsis Fabricius, 1805: 80 . Type locality: “America meridionali.” SYNTYPE : 1 ♀ [The Natural History Museum of Denmark , Copenhagen, Denmark , UZMC] (original description). Cyphomyia fenestrata Macquart, 1846: 48 . Type locality: Mexico , Yucatán , Merida Syntypes ♀ , J [unknown depository institution] (synonym). Stratiomys albitarsis : WOODLEY (2001: 158 , 2011: 410 ). Material examined. COLOMBIA : Caldas ,Anserma,Vereda San Pedro, Finca La Marqueza, trunk of papaya, 1700 m , A. L. Montoya leg. Eight larvae collected on 6.i.2015 , five emerged on 9.ii.2015 . Puparia and adults: 3 ♀♀ (CEUA-109379, CEUA-109376, CEUA-109377); 2 JJ (CEUA-109378, CEUA-83782); 1 puparium not emerged into adult (CEUA-109375); 2 last instar larvae not emerged into adult (CEUA- 83967, CEUA-109381). Description. Puparium ( Figs 3–4 ). Length 14.2– 17.0 mm, comprising head ( Fig. 3 ), three thoracic segments ( Figs 3–4 ) and eight abdominal segments ( Fig. 4 ); cuticle brownish, erected dorsal setae mostly pubescent. Head ( Fig. 3 ). Subtriangular, roughly as long as one tenth of entire puparium length, longer than wide. Labrum triangular. Antenna short, rising dorsolaterally in anterior part of head. Eyes prominent, arising in middle of head capsule; lateral setae bipartite at apex; bulbous tubercles posterior to each eye. Mandibular-maxilary complex well developed; ventrocraneal sulcus evident. Chaetotaxy: 2 pairs of labral setae (Lb), 2 pairs of clypeofrontal setae (Cf), 1 pair of dorsolateral setae (Dl), 2 pairs of lateral setae (L) and 3 pairs of ventral setae (V). Thorax. Prothorax subrectangular with anterior spiracles prominent; mesothorax and metathorax rectangular with curved side edges. Chaetotaxy: prothorax in dorsal view with 2 pairs of anterodorsal setae (Ad), 3 pairs of dorsal setae (D1) and 1 pair of dorsolateral setae (Dl). Ventrally, 1 pair of ventrolateral setae (Vl) and 2 pairs of ventral setae (V), one small and simple (V 1), and one strong and tripartite (V 2) ( Fig. 3 ); mesothorax in dorsal view with 1 pair of anterodorsal setae (Ad), 1 pair of dorsal setae (D) and 1 pair of dorsolateral setae (Dl). Ventrally, 1 pair of ventrolateral setae (Vl) and 2 pairs of ventral setae (V), one tiny and simple (V 1), and one tripartite (V 2); metathorax in dorsal view with 2 pairs of anterodorsal setae (Ad), 1 pair of dorsal setae (D), 1 pair of dorsolateral setae (Dl) and 1 pair of lateral setae (L). Ventrally, 1 pair of ventrolateral setae (Vl) and 2 pairs of ventral setae (V), one small and simple (V 1), and one tripartite (V 2) ( Fig. 4 ). Abdomen ( Fig. 4 ). Pupal respiratory horn well preserved, arising from segments 2–5; segments 1–7 similar in shape. Dorsally with 3 pairs of dorsal setae (D), 1 pair of dorsolateral setae (Dl) and 1 pair of conspicuous and long lateral setae (L). Ventrally, with 2 pairs of ventrolateral setae (Vl) and 3 pairs of ventral setae (V), with (V 1) shorter than (V 2) and (V 3). Segment 6 with elliptical sternal patch. Segment 8 semicircular in shape, exhibits posterior spiracle dorsally just before apex (Ps); anal slit (Asl) much shorter than segment 8, surrounded by two perianal grooves. Chaetotaxy: dorsally with 1 pair of dorsal setae (D) and 2 pairs of lateral setae (L). Ventrally, 4 pairs of ventral setae (V), 1 pair of subapical setae (Sa) and 1 pair of apical setae (Ap). Comments. As in other described puparia of Stratiomyidae species, the features of the mature larvae are still evident, except for the emerging suture well prominent and the presence of pupal respiratory horns. Only in one puparium, some of the horns did not reach full length. Fig. 3. Puparium of Cyphomyia albitarsis ( Fabricius, 1805 ) , capsule cephalic and thoracic segment one. Left, dorsal view; right, ventral view. Scale bar = 1 mm. Fig. 4. Puparium of Cyphomyia albitarsis ( Fabricius, 1805 ) , thoracic segments two and three, and abdominal segments. Left, dorsal view; right, ventral view. Scale bar = 1 mm. Fig. 5. Adult of Cyphomyia albitarsis ( Fabricius, 1805 ) (♀), habitus, lateral view. Table 1. Comparison between the known immature stages of nine Cyphomyia species.

Species	Size (mm)	Head	Thorax	Abdomen	Reference
C. albitarsis	14.2–17.0	Head longer than wide.	Prothorax with 3 pairs	Abdominal segments 1–7 with 1 pair	Present work
Ocular region with	of dorsal setae (D) and	of dorsolateral setae.
one bulbous tubercle	2 pairs of ventral setae	Arrangement of dorsal setae (D)
posterior to each eye.	(V).	displaced from the center to the
Pro-, meso- and meta-	posterior margin. Arrangement of
thorax with ventral seta	ventral setae (V) almost in the same
(V2) tripartite.	plane.
Setation mostly pubes-	Setation mostly pubescent.
cent.
C. aurifrons	~16.0	Head short, moderate-	Prothorax with 3 pairs	Abdominal segments 1–7 with 2	XEREZ et al. (2004)
ly flat.	of anterodorsal setae	pairs of dorsolateral setae (D).
Posterior area of eyes	(Ad).
without tubercles.	Pro-, meso- and meta-
thorax with ventral setae
(V2) tripartite
C. bicarinata	~11.7	Head longer than wide.	Prothorax with 1 pair of	Abdominal segment 1 with 2 pairs	MCFADDEN & JAMES
excluding	Ocular tubercles presu-	dorsolateral setae (Dl),	of dorsal setae (D), the inner longer	(1969)
head and	mably absent.	2 pairs of anterodorsal	than the outer seta.
prothorax	setae and 2 pairs of	Abdominal segment 8 with 1 pair
dorsal setae (D).	of dorsal setae (D), 2 pairs of lateral
setae (L) and 1 pair of apical setae
(Ap).
C. erecta	~13.3	Head longer than wide.	Prothorax with 1 pair of	Abdominal segment 1 with 2 pairs	MCFADDEN & JAMES
excluding	Ocular tubercles	dorsolateral setae (Dl)	of dorsal setae (D) of similar length.	(1969)
head and	presumably present an-	and 3 pairs of dorsal	Abdominal segment 8 with 1 pair
prothorax	teriorly and posteriorly	setae (D), dorsal integu-	of dorsal setae (D), 1 pair of lateral
of each eye.	ment with small, dark	setae (L) and one pair of apical setae
transverse plates arran-	(Ap).
ged in linear pattern
near lateral margin.
C. leucocephala	~14.0	Head longer than wide.	Prothorax with ventral	Abdominal segments 1–7 with 3	IIDE (1963) ; XEREZ et
Ocular region with	setae (V) bipartite.	pairs of dorsal setae (D) and 2 pairs	al. (2004)
one bulbous tubercle,	of dorsolateral setae (Dl); ventrally,
immediately posterior	3 pairs of ventral setae (V) and 2
each eye.	pairs of ventrolateral setae (Vl).
C. marginata	~20.1	Head longer than wide.	Not mentioned.	Abdominal segment 1 with 3 pairs	MCFADDEN (1967) ;
of dorsal setae (D), decreasing in	XEREZ et al. (2004)
length towards the most distal one.
Abdominal segment 8 with long anal
slit (As) ventrally, 2 pairs of lateral
setae (L), 4 pairs of ventral setae (V)
and 2 pairs apical setae (Ap).
C. picta	~15.0	Head elongated and	Prothorax with 3 pairs	Abdominal segments 1–7 with 2	XEREZ et al. (2004)
projected forward.	of anterodorsal setae	pairs of dorsolateral setae (Dl).
Posterior area of eyes	(Ad).
without tubercles.	Pro-, meso- and meta-
thorax with ventral setae
(V2) tripartite.
C. pilossisima	~18.0	Head short, only	Prothorax with 2 pairs	Segments 1–7 with 1 pair of dorsola-	JAMES (1957)
slightly longer than	of anterodorsal setae	teral setae (Dl).
wide.	(Ad) and 2 pairs of
Ocular area with two	dorsal setae (D).
bulbous tubercles pos-
teriorly of each eye.
C. souzalopesi	8.5–16.5	Head longer than wide,	Setation densely plumose.		IIDE (1967)
ocular region without
tubercles

The body shape, mosaic appearance of integument and general chaetotaxy present in Cyphomyia albitarsis described here are similar to the other species, but some marked differences were observed. The arrangement of setae on the prothorax of C. albitarsis differs from C. aurifrons and C. picta in the number of anterodorsal setae, with 2 pairs in C. albitarsis and 3 pairs in the other two. C. pilossisima differs in having 2 bulbous tubercles behind each eye and prothorax with only 2 pairs of dorsal setae. Some setae in C. albitarsis have a brushy appearance, being sometimes bipartite at apex or tripartite in the entire length, and pubescent, although they never show a plume-like appearance as seen in C. souzalopesi described by IIDE (1967) . Additional external features are compared with the other known species of the genus in Table 1 . Distribution. Neotropical: Bolivia , Brazil , Colombia , Costa Rica , Ecuador , Guyana , Honduras , Mexico , Panama , Paraguay , Peru , Trinidad , Venezuela ( WOODLEY 2001:158 ).