Two new species of Cyclocybe (Agaricales, Tubariaceae) from China Author Zhu, Xin-Yu 0000-0001-8654-7485 State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand & zhuxinyu 2112356 @ gmail. com; https: // orcid. org / 0000 - 0001 - 8654 - 7485 zhuxinyu2112356@gmail.com Author Nyima, Tenzin 0009-0002-9337-6877 Tibet Plateau Institute of Biology, Lhasa 850000, People’s Republic of China & danzeng 220227 @ 163. com; https: // orcid. org / 0009 - 0002 - 9337 - 6877 Author Vizzini, Alfredo 0000-0001-8390-6446 Dipartimento di Scienze Della Vita e Biologia Dei Sistemi, Università di Torino, Viale P. A. Mattioli 25, 10125 Torino, Italy & alfredo. vizzini @ unito. it; https: // orcid. org / 0000 - 0001 - 8390 - 6446 alfredo.vizzini@unito.it Author He, Mao-Qiang 0000-0002-9300-7484 State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China & hemaoqiangleo @ gmail. com; https: // orcid. org / 0000 - 0002 - 9300 - 7484 hemaoqiangleo@gmail.com Author Phurbu, Dorji 0000-0003-3001-5740 Tibet Plateau Institute of Biology, Lhasa 850000, People’s Republic of China & puduo @ 126. com; https: // orcid. org / 0000 - 0003 - 3001 - 5740 Author Zhao, Rui- Lin 0000-0003-4028-7934 State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China & College of Life Sciences, University of Chinese Academy of Sciences, Huairou District, Beijing, 100408, People’s Republic of China & zhaorl @ im. ac. cn; https: // orcid. org / 0000 - 0003 - 4028 - 7934 zhaorl@im.ac.cn text Phytotaxa 2023 2023-10-12 620 1 33 46 http://dx.doi.org/10.11646/phytotaxa.620.1.3 journal article 273177 10.11646/phytotaxa.620.1.3 04fac18b-5aeb-4ddf-9eb4-b8bd6f6418ab 1179-3163 10015455 Cyclocybe dinggyensis R.L. Zhao & X.Y. Zhu sp. nov. Fungal Names No: 571269 FoF number: 13982 Figs 3C, D , 5 FIGURE 4. Microscopic features of Cyclocybe suberebia (HMAS 286977, holotype) A, Basidiospores; B, Basidia; C, Pleurocystidia; D, Cheilocystidia; E, Pileipellis hymeniphorm. FIGURE 5. Microscopic features of Cyclocybe dinggyensis (HMAS 286979, holotype) A, Basidiospores B, Basidia C, Pleurocystidia D, Cheilocystidia E, Pileipellis hymeniphorm. Diagnosis . Basidioma slender. It differs from C. erebia in the number of spores, C. erebia is bisporic, C. dinggyensis is tetrasporic. It differs from C. erebioides in annulus, C. erebioides with a wide and complex membranous annulus, C. dinggyensis with a simple membranous annulus, and C. erebioides with clamp-connections, but C. dinggyensis clampless hyphae. Etymology: the epithet “dinggye” refers to Dinggyê County where the type specimen is from. Description. Basidiomes small. Pileus 20 mm in diam., broadly convex to convex, not distinctly depressed or umbo, hygrophanous, viscid, glabrous, brown to darker brown, generally darker in the center and almost brown in the margin, margin distinctly striate, not involute. Context thin, up to 1 mm , concolorous with pileus surface. Lamellae subdecurrent, thick, crowded, with series of 1–3 lamellulae, light brown. Stipe 43 × 4 mm , central, cylindrical, hollow, dry, fibrous, with annular zone, concolorous with pileus surface, daker at the base. Annulus single, concolorous with lamellae, membranous, persistent, upturned. Odor not distinctive. Taste indistinct. Basidiospores 7.4–9.2 × 3.6–5.2 μm, [x = 8.4 ± 0.4 × 4.4 ± 0.4, Q = 1.7–2.2, Q m = 1.9 ± 0.1, n =20], oblong, subellipsoid to subamygdaliform,smooth, thick-walled, some oil-like droplets, no germ pore, brownish to brown in. Basidia 22.4–35.6 × 6.2–8.8 μm, clavate, 4-spored, most middle constrained, thin walled, hyaline. Pleurocystidia 46.7–59.7 × 9.2–13.0 μm, generally utriform, sometimes cylindrical, thin-walled, hyaline, numerous. Cheilocystidia 26.7–42.7 × 7.3–13.2 μm, similar to pleurocystidia but lacking cylindrical, mostly utriform, thin walled, hyaline. Hymenophoral trama subregular, consisting of 3.0–7.0 μm-wide hyphae. Pileipellis hymeniform formed by vesicular to clavate elements, 11.2–23.6 × 6.7–11.1 μm. Pileocystidia not observed. Caulocysidia not observed. Clamp-connections absent. Habitat, ecology and distribution. Solitary on the ground, known from Tibet , China ; from July to October. Material examined. China . Tibet : Shigatse , Dinggyê county ( 27°55’15” N , 87°21’37” E ), alt. 3060 m , 29 July 2022 , collected by Mao-Qiang He , Bin Cao , Jia-Xin Li. ZRL20220414 ( Holotype : HMAS 286979 ) .