Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China Author Huang, Shengting College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China Author Xia, Jiwen https://orcid.org/0000-0002-7436-7249 College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China Author Zhang, Xiuguo Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China Author Sun, Wenxiu College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China text MycoKeys 2021 2021-02-19 78 49 77 http://dx.doi.org/10.3897/mycokeys.78.60878 journal article http://dx.doi.org/10.3897/mycokeys.78.60878 1314-4049-78-49 5BCC2C78F2F856C3B93134F805790F02 Diaporthe osmanthi H. Long, K.D. Hyde, & Yong Wang bis, MycoKeys 57: 120. (2019) Figure 7 Description. Conidiomata pycnidial, globose, 5-10 pycnidia grouped together, dark brown to black, exuding creamy to yellowish conidial droplets from central ostioles. Conidiophores hyaline, smooth, densely aggregated, branched, cylindric-clavate, 20.5-32.0 x 1.8-2.4 μm . Conidiogenous cells phialidic, hyaline, terminal, cylindrical, straight, 14.0-20.5 x 1.5-2.0 μm , tapered towards apex. Alpha conidia hyaline, aseptate, fusiform, tapering towards both ends, guttulate, 7.3-9.3 x 1.8-2.3 μm (mean = 8.5 x 2.0 μm , n = 20). Beta conidia hyaline, aseptate, filiform, curved, 22.0-28.5 x 1.0-2.0 μm (mean = 27.2 x 1.3 μm , n = 20). Gamma conidia not observed. Sexual morph not observed. Culture characteristics. Cultures incubated on PDA at 25 °C in darkness, growth rate 12.0-13.5 mm diam/day, cottony with abundant aerial mycelium, sparse at margin. With several concentric rings of dense hyphae, white on surface, white to pale brown on reverse. Specimen examined. China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 19 April 2019, S.T. Huang. On diseased leaves of Litchi chinensis ( Sapindaceae ) HSAUP194.21, living culture SAUCC194.21. Figure 7. Diaporthe osmanthi (SAUCC194.21) a infected leaf of Litchi chinensis b, c surface and reverse of colony after 15 days on PDA d conidiomata e-g conidiophores and conidiogenous cells h, i beta conidia j, k alpha conidia. Scale bars: 10 μm ( e-k ). Notes. Diaporthe osmanthi was originally described from the leaves of Osmanthus fragrans ( Oleaceae ) in Guangxi province, China ( Long et al. 2019 ). In the present study, phylogenetic analyses (Fig. 1 ) indicated that the strain SAUCC194.21 is closely related to Diaporthe osmanthi and D. podocarpi-macrophylli ( Gao et al. 2017 ). Morphological comparison indicated that this strain was most similar to D. osmanthi by the size of alpha conidia and beta conidia. We therefore identify this strain as belonging to D. osmanthi .