Revision of the genus Neozavrelia Goetghebuer, Thienemann, 1941 (Diptera Chironomidae) from Eastern Siberia and the Russian Far East, with the description of new species Author Orel, Oksana V. text Zootaxa 2021 2021-03-01 4938 3 251 297 journal article 7894 10.11646/zootaxa.4938.3.1 02750558-eeb0-417a-813c-7c34b00a1979 1175-5326 4569580 872AB368-FAFB-499B-B3E5-FB08BAC911A7 Neozavrelia kuzmychikha sp. nov. ( Figs 39–52 ) zoobank.org:act: 2FD6C0BF-C25C-4F48-BE48-4454355CE358 Type material. Holotype : male, RUSSIA , KHABAROVSK TERRITORY, basin of the Bureysky Reservoir , Kuzmichiha River , 9 km from the estuary, 21.vii.2005 , leg. E. Makarchenko. Paratype : 1 male , 1 pupa , same data, 21.vii.2005 , leg. E. Makarchenko. Derivatio nominis. From the type locality Kuzmichiha River. Diagnosis. Male: darkly coloured species, with wing 1.8 mm long and AR 0.75–0.80; ultimate flagellomere 376–384 μm. Length of 2–4 flagellomeres of maxillary palpomeres 416–440 μm; 2 nd palpomere swollen. R with 11–15, R 1 with 1–2, R 4+5 with 2, M 1+2 with 18 setae, M 3+4 , Cu 1 and An without setae. LR PI 1.36–1.50. Basitarsus of mid leg with 2–3 sensilla chaetica. Mid tibia with 1 spur.Anterior margin of tergite IX triangular form with well developed lateral lobes.Anal point slender, with narrowed and strongly elongated tip. Superior volsella oval-shaped, bearing 3 strong apical and 8–9 fine dorsal setae, microtrichia present. Stem straight and parallel-sided, 27–34 μm long. Pupa: thoracic horn fusiform with a pointed apex, without spines, 146 μm long; base of thoracic small, rounded, diameter 17 μm. Abdomen 2.5 mm long; hook row with 130 spines; tergites II–III with band of pale shagreen in the anterolateral corners; tergite VII without shagreen; anal comb with dark 11 apical and 18 dorsal teeth; anal lobe with dark strong spines on posterior third and 16 brown lamelliform setae; on the conjunctives of segments III/IV and V/VI present double plumose or simple setae. FIGURES 39–42. Details of the structure of the male of Neozavrelia kuzmychikha sp. nov. 39—maxillar palp; 40—total view of hypopygium; 41—superior volsella, dorsal view; 42—thoracic horn. Scale bar 50 μm. FIGURES 43–48. The male of Neozavrelia kuzmychikha sp. nov. 43—head, dorsal view; 44—antenna; 45—apical part of mid tibia; 46—tibia and basitarsus of fore leg; 47, 48—hypopygium. Scale bar 50 μm. Description . Adult male (n=2). Total length 2.2 mm ; wing length 1.8 mm . Total length/wing length 1.2. Colouration . Ground colour of thorax, scutellum, maxillary palpomeres and abdomen, legs yellowish brown; halters yellowish; antenna, scutal stripes, median band on distal half of mesonotum and postnotum dark brown. Head (39, 43–44). Frontal tubercles reduced in the form of small spots 3–7 μm in diameter, situated on swellings of frontal lobes. Temporal setae 8–12. Clypeus with 15–20 setae. Antenna with 10 flagellomeres, 864–880 μm long; ultimate flagellomere 376–384 μm. AR 0.75–0.80. Maxillary palpomeres 2–4 combined 416–440 μm long, their individual lengths (in μm): 48: 96: 112: 184; second palpomere swollen. Antenna length/palp length 2.0–2.1. Thorax . Acrostichals 10–14, dorsocentrals 6–7, prealars 1. Scutellum with 9 setae. Wing width 0.64 mm . VR 1.18. R with 11–15, R 1 with 1–2, R 4+5 with 2, M 1+2 with 18 setae, M 3+4 , Cu 1 and An without setae. Cell r 2+3 , r 4+5 with setae. Brachiolum with 1 seta. Legs (see Table 5 ). Apical curved tibial spur of fore leg 34–41 μm long ( Fig. 46 ). Combs of mid and hind tibiae separated; mid tibia with 1 spur ( Fig. 45 ), 20–31 μm long; on hind tibia each comb bears straight or slightly curved spur, 34–41 μm long. Basitarsus of mid leg with 2–3 sensilla chaetica. TABLE 5 . Lengths (in µm) and proportion of legs of N. kuzmychikha sp. nov.

P	f	t	ta1	ta2	ta3	ta4	ta 5
P1	714–735	762	630–693	420	273–294	189–210	126
P 2	777	588–630	252–294	168–189	126–137	95–116	95–116
P3	798	630–756	378–420	273	231	147	126

TABLE 5. (Continued)

P	LR	SV	BV	BR
P 1	1.36–1.50	1.70–1.90	1.78–1.81	2.5–3.0
P2	0.43–0.47	4.79–5.42	3.18–3.28	3.3–5.6
P3	0.50–0.67	3.40–4.11	2.35–2.49	7.2

Hypopygium ( Figs 40–41 , 47–48 ). Anal tergite with posterolateral lobes 24–27 μm long in dorsal view. The anal point stout, parallel-sided, rounded apically, dorsally cover with small spinulae and bearing 5–7 lateral setae on each side of anal point, 34 μm long and 17 μm wide on dorsal view. Gonocoxite 155 μm long, along the inner margin with 4–5 setae. The width of the transverse sternapodema 61 µm . Superior volsella oval-shaped (44–51 μm long, 20 μm wide), bearing 3 strong apical and 8–9 fine dorsal setae, microtrichia present. Digitus 34 μm long and 10 μm wide. Stem of median volsella straight and parallel-sided, 27–34 μm long and 14 μm wide, bearing slender lamelliform setae; total length of median volsella 71–85 μm. Inferior volsella curved and rounded apically, 119 μm long, with 14–18 setae. Gonostylus straight and stout, 102 μm long and expanded at about median (31–51 μm). HR 1.50–1.53. Pupa (n=1, male). Cephalothorax ( Figs 42 , 49 ). Head lost. Thoracic horn fusiform with a pointed apex, without spines, 146 μm long and 34 μm wide; base of thoracic small, rounded, diameter 17 μm. Antepronotals 2 (1 median 119 μm long and 1 lateral seta broken). Precorneals 3 (1st 153 μm long, 2nd 119 μm long, and 3rd seta lost). Dorsocentrals 4 (1 st pair 51 μm long; 2 nd pair 85 μm long); distance between Dc2 and Dc3 238 μm. Abdomen 2.5 mm long ( Figs 50–52 ). Hook row 400 μm long with 130 spines on tergite II. Tergites II–VI with a pair of longitudinal oval patches of dark spines on anterior part. Length longitudinal patches on tergites II–VI (in μm): 119: 143: 160: 170: 170. Tergites II–III with band of pale shagreen in the anterolateral corners. Tergite VII without shagreen, VIII and IX with a pair patches of shagreen medially. Comb of segment VIII 78 μm wide, with dark 11 apical and 18 dorsal teeth. Anal segment 323 μm long and 306 μm wide, each anal lobe with dark strong spines on posterior third and 16 brown lamelliform setae. Double plumose or simple setae visible on the conjunctives of segments III/IV and V/VI ( Fig. 50 ). Segments II–VII with 3 simple L setae (on segment VII 2th L seta longest and stronger), VIII with 1 L seta, IX with 2 pair lamelliform setae. Larva unknown. Remarks. The males of N. kuzmychikha sp. nov. the structure of the hypopygium is most similar to N. kedrovaya sp. nov. , but differ well in the following combination of features: the length of the antenna and maxillary palp, wing chaetotoxia, and the presence of only one spur on the middle tibia (see Table 6 ). TABLE 6. The males of genus Neozavrelia Goetghebuer & Thienemann, 1941 from Eastern Siberia and the Russian Far East

Characters	N. kedrovaya sp. nov. (n=15)	N. kuzmychikha sp. nov. (n=2)	N. nadezhdae sp. nov. (n=6)	N. paramushirica sp. nov. (n=3)	N. minuta ( Linevich, 1963 ) our data (n=3)	N. yakuefea ( Sasa, Suzuki, 2000 ) our data (n=3)
Total length, mm	2.5–3.0	2.2	2.4	2.0	2.8–3.4	1.7–1.9
Wing length, mm	1.8–2.4	1.8	1.80–1.88	1.72–1.80	2.60–2.72	1.3–1.4
Frontal tubercles length, µm	absent or ø 3	ø 3–7	ø 3–7	absent or ø 3	absent	absent
Maxillar palp length, µm	504–624	416–440	424–480	416–464	600–656	401–449
Form of 2nd palpomere	parallel-sided	swollen	parallel-sided	parallel-sided	parallel-sided	parallel-sided
Antenna length, µm	928––1132	864–880	824–1056	792–832	1192–1256	640–760
Ultimate flagellomere, μm	408–600	376–384	280–480	288–312	600–664	184–320
The tip of the antenna	normal	normal	normal	slightly swollen	normal	swollen
AR	0.79–1.15	0.75–0.80	0.51–1.13	0.56–0.61	0.99–1.14	0.40–0.73
Ac	11–20	10–14	10–16	13–16	17–22	10–12
Dc	6–12	6–7	7–12	5–10	10–13	5–8
Scts	6–13	9	9–12	6–12	8–11	4–9
R	17–29	11–15	13–26	14–18	17–21	9–17
R1	8–14	1–2	4–14	2–4	0–4	3–7
R4+5	12–25	2	5–14	3–7	11–16	5–7
M1+2	16–50	18	20–40	20–32	20–26	16–25
M3+4	3–16	0	15–24	1–6	4–6	5–16
Cu1	0–11	0	2–14	0	0	0–2
An	0––17	0	0–25	0	0–9	0
LR1	1.30–1.50	1.36–1.50	1.17–1.48	1.41–1.52	1.27–1.37	1.46–1.61
BR1	2.5–4.5	2.5–3.0	2.86–2.88	2.13–3.00	2.0–2.8	2.5–3.0
Number spurs on tP2	2	1	2	2	2	2
Number of sensilla chaetica on ta1P2	2–5	2–3	1–5	0	2–4	0

......continued on the next page TABLE 6. (Continued)

Characters	N. kedrovaya sp. nov. (n=15)	N. kuzmychikha sp. nov. (n=2)	N. nadezhdae sp. nov. (n=6)	N. paramushirica sp. nov. (n=3)	N. minuta ( Linevich, 1963 ) our data (n=3)	N. yakuefea ( Sasa, Suzuki, 2000 ) our data (n=3)
Anterior margin of tergite IX	triangular	triangular	straight	triangular	triangular	straight
Anal point length, µm	34–61	34	44–51	14–24	48–51	27–34
Anal point width (median), µm	17–24	17	24	24	17	17
Superior volsella length, µm	48–58	44–51	51–71	44–54	51–54	34–51
Superior volsella width, µm	27–37	20	17–31	17	27–31	14
Microtrichia on superior volsella	absent/present	present	absent	present/absent	present	absent
Digitus length, µm	27–34	34	34–37	34	34	24–34
Stem of median volsella length, µm	34–48	27–34	27–44	34–41	44–51	24–37
Gonocoxite length, µm	146–170	155	126–153	126–150	194–238	119–122
Gonostylus length, µm	102–129	102	102–136	95–102	133–150	78–88
HR	1.26–1.67	1.50–1.53	1.13–1.43	1.23–1.57	1.41–1.66	1.39–1.52

Pupa of N. kuzmychikha sp. nov. is most similar to the pupa of N. kedrovaya sp. nov. , but differs well from the latter fusiform thoracic horn and the presence of double plumose or simple setae on the conjunctives of segments III/IV and V/VI, and also the presence of 1 lateral seta on segment VIII, medially located shagreen on tergite VIII. Whereas, the pupa of N. kedrovaya sp. nov. has a triangular thoracic horn and the conjunctives without shagreen, tergite VIII without lateral setae and shagreen on it located in the proximal part (see Table 10 ). Distribution. Russian Far East ( Khabarovsk Territory).