Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species Author Li, A. - H. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China & China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China Author Yuan, F. - X. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China & North Minzu University, Yinchuan, Ningxia, 750030, China Author Groenewald, M. Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands Author Bensch, K. Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands Author Yurkov, A. M. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, 38124, Germany Author Li, K. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China Author Han, P. - J. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China Author Guo, L. - D. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China Author Aime, M. C. Purdue University, Department of Botany and Plant Pathology, West Lafayette, IN, 47901, USA Author Sampaio, J. P. UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal & PYCC - Portuguese Yeast Culture Collection, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal Author Jindamorakot, S. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand Author Turchetti, B. Department of Agriculture, Food and Environmental Sciences & Industrial Yeasts Collection DBVPG, University of Perugia, Perugia, 74 - I- 06121, Italy Author Inacio, J. School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, BN 2 4 GJ, UK Author Fungsin, B. TISTR Culture Collection, Thailand Institute of Scientific and Technological Research (TISTR), 35 M 3, Technopolis, Khlong Ha, Khlong Luang, Pathum Thani, 12120, Thailand Author Wang, Q. - M. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China & College of Life Sciences, Hebei University, Baoding, Hebei Province, 071002, China wangqm@im.ac.cn Author Bai, F. - Y. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China baify@im.ac.cn text Studies In Mycology 2020 2020-01-28 96 17 140 journal article 10.1016/j.simyco.2020.01.002 PMC7082220 32206137 10497182 Genolevuria pseudoamylolytica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828740. Fig. 7F . Etymology : the specific epithet pseudoamylolytica refers to the similar colony morphology to that of Genolevuria amylolytica . Culture characteristics : In YM broth, after 7 d at 17 °C, cells are ellipsoidal and subglobosal, 2.9– 5.2 × 3.3– 7.7 μm and single, budding is polar ( Fig. 7F ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, mucoid, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced. Physiological and biochemical characteristics : Glucose fermentation is absent. Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin (weak), soluble starch, D-xylose (weak), Larabinose, D-arabinose, D-ribose, L-rhamnose (weak), Dglucosamine, N-Acetyl-D-glucosamine, D-mannitol (weak), Dglucitol (weak), Methyl-α- D-glucoside and salicin are assimilated as sole carbon sources. Methanol, ethanol, glycerol, erythritol, ribitol, galactitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive. Physiologically, G. pseudoamylolytica differs from the two closely related species, G. amylolytica and G. tibetensis , in its inability to assimilate ribitol and succinate and the ability to assimilate L-sorbose and potassium nitrate ( Table S1.2 ). Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443 T ; (C, D) Koc. ischaemi CGMCC 2.3565 T ; (E) Koc. nitrophila CGMCC 2.3465 T ; (F) G. pseudoamylolytica CGMCC 2.5809 T ; (G) Tr. shuangheensis CGMCC 2.5615 T ; (H) V. melezitolyticum CGMCC 2.3472 T ; (I) V. pseudopenaeus CGMCC 2.3165 T ; (J) V. europaea CGMCC 2.3099 T ; (K) Ca. follicola CGMCC 2.3447 T ; (L) Ca. simaoensis CGMCC 2.3580 T ; (M) Kwoni. ovata CGMCC 2.3439 T ; (N) Te. helanensis CGMCC 2.4450 T ; (O) Te. globosa CGMCC 2.5648 T ; (P) Te. korlaensis CGMCC 2.3835 T . Bars = 10 μm. Typus : China , Daliangzi river national forest park , Heilongjiang province , obtained from a leaf of an unidentified plant, Aug. 2014 , Q.-M. Wang ( holotype CGMCC 2.5809 T preserved in a metabolically inactive state, ex-type CBS 13955 = HLJ1B6).