Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China Author Zhang, Zhaoxue Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Author Liu, Rongyu Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Author Liu, Shubin Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Author Mu, Taichang Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Author Zhang, Xiuguo Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China Author Xia, Jiwen https://orcid.org/0000-0002-7436-7249 Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China zhenjunxue@126.com text MycoKeys 2022 2022-04-14 88 171 192 http://dx.doi.org/10.3897/mycokeys.88.82229 journal article http://dx.doi.org/10.3897/mycokeys.88.82229 1314-4049-88-171 298CC096859B58659552F4D3388873F6 Pestalotiopsis licualicola K. Geng, Y. Song, K.D. Hyde & Yong Wang bis, Phytotaxa 88 (3):51. (2013) Fig. 5 Description. Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on PDA: Conidiomata solitary, scattered, black, raising above surface of culture medium, subglobose. Conidiophores cylindrical, hyaline, smooth-walled. Conidiophores often indistinct. Conidiogenous cells discrete, hyaline, simple, filiform, 5.5-10.0 μm long. Conidia 18.0-24.5 x 4.0-5.5 μm , mean +/- SD = 20.5 +/- 1.9 x 5.3 +/- 0.3 μm , fusiform, straight to slightly curved, 4-septate, smooth, greyish brown; basal cell conical, hyaline, thin-walled, 2.8-6.0 μm long; with three median cells, dark brown, concolorous, septa and periclinal walls darker than the rest of the cell, together 11.5-16.0 μm long, mean +/- SD = 13.2 +/- 1.2 μm ; second cell from base 3.4-5.5 μm ; third cell 3.3-4.7 μm ; fourth cell 3.5-5.1 μm ; apical cell hyaline, conic to subcylindrical, 3.1-5.3 μm ; with 1-3 tubular apical appendages (mostly 1) without knobs, arising from the apex of the apical cell, 10.0-20.5 μm long, mean +/- SD = 16.0 +/- 4.0 μm ; basal appendage filiform, short. Figure 5. Pestalotiopsis licualicola (SAUCC210087) a diseased leaf of Ilex chinensis b surface of colony after 7 days on PDA c reverse of colony after 7 days on PDA d conidiomata f, g, j, k conidiogenous cells with conidia e, h, i, l, m conidia. Scale bars: 10 μm ( e-m ). Culture characteristics. Colonies on PDA reaching 70.0-80.0 mm diam after 7 d at 25 °C, growth rate 9.0-12.0 mm/day, edge entire, whitish to pale honey coloured, with sparse aerial mycelium on the surface, with black, gregarious conidiomata; reverse similar in colour. Specimen examined. China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of Ilex chinensis , HSAUP210087, living culture SAUCC210087; on diseased leaves of Ilex chinensis , HSAUP210088, living culture SAUCC210088. Notes. In the present study, two strains (SAUCC210087 and SAUCC210088) from symptomatic leaves of Ilex chinensis were clustered to Pestalotiopsis licualicola clade ( Geng et al. 2013 ) based on phylogeny (Fig. 1 ). Morphologically, our strains were the same as P. licualicola , which was originally described with an asexual morph on leaves of Licuala grandis in China. The sexual morph of P. licualicola was undetermined yet. This is the first time this species has been reported in Ilex chinensis ( Aquifoliaceae ) in China.