A new species of Bambuina de Mello, Horta & Bolfarini, 2013 (Orthoptera: Grylloidea: Phalangopsidae: Luzarinae) from the Parque Nacional do Itatiaia, Brazil Author Souza-Dias, Pedro G. B. 0000-0002-7130-1324 Departamento de Entomologia, Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, São Cristóvão, Rio de Janeiro, RJ, Brazil. pedrogdias @ gmail. com; https: // orcid. org / 0000 - 0002 - 7130 - 1324 pedrogdias@gmail.com Author Borille, Maria V. A. 0000-0001-9827-8454 Departamento de Entomologia, Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, São Cristóvão, Rio de Janeiro, RJ, Brazil. & mvaborille @ gmail. com; https: // orcid. org / 0000 - 0001 - 9827 - 8454 mvaborille@gmail.com Author Campos, Lucas Denadai De 0000-0003-2922-1571 Departamento de Zoologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, travessa 14, n. 101, 05508 - 090, Cidade Universitária, São Paulo, SP, Brazil. lcdenadai @ gmail. com; https: // orcid. org / 0000 - 0003 - 2922 - 1571 lcdenadai@gmail.com text Zootaxa 2022 2022-11-30 5214 1 130 140 journal article 199051 10.11646/zootaxa.5214.1.6 c8c70b7a-1200-4308-9d7f-ecdd6f08c93f 1175-5326 7382079 1457EBE2-5B38-419A-8963-65DF7EF9A262 Bambuina zikani Souza-Dias & Borille n. sp. Figs. 1–4 Etymology. Species named after Joseph Francisco Zikán (1881–1949), a Czech entomologist who greatly contributed to the knowledge of the Brazilian insect fauna in the first half of the last century. Zikán conducted expeditions to collect insects in several localities of the Atlantic Forest, mainly in the Parque Nacional do Itatiaia (Itatiaia National Park) area, where he worked as a naturalist for many years. During his lifetime, Zikán assembled large collections, mainly Lepidoptera, Coleoptera, and Hymenoptera, which are now housed at the Instituto Oswaldo Cruz (IOC) ( Rio de Janeiro ). Type locality. Brazil , Rio de Janeiro , Itatiaia . Type material. Holotype , allotype , and paratypes : 5 males and 4 females ( MNRJ ) ; 1 male and 1 female ( MZSP ) . Holotype : Brasil , R[io de]J[aneiro], Itatiaia, P [arque]N[acional] do Itatiaia / Ao longo da estrada, entre o Brejo da Lapa e a Casa de Pedra — 2100 m / 20.X.2018 / Souza-Dias , Redu , & Campos col. | LDC_150 | MNRJENT6-29221 ( MNRJ ), condition: one foreleg removed for DNA preservation, one foreleg detached, specimen dissected . Allotype : P[arque]N[acional] do Itatiaia / Brasil , R[io de]J[aneiro], Itatiaia / Escadaria da Piscina Natural do Maromba / Alt. 1000 m / 22°26′09.1″S , 44°37′31.2″W / 12–18.III.2017 / L.D. Campos ; S.S. Nihei ; F.M. Gudin ; D.M.A. Garcia ; R.P.V. Dios col. | PSD 155 | MNRJ-ENT6-29214 ( MNRJ ), condition: one foreleg removed for DNA preservation, specimen dissected . Paratype males. (1) Same data as for holotype | PSD 488 |MNRJ-ENT6-29219 ( MNRJ ), condition: one foreleg removed for DNA preservation, one foreleg missing, specimen dissected; (2) Same data as for allotype | PSD 152 |MNRJ-ENT6-29218 ( MNRJ ), condition: one foreleg removed for DNA preservation, one foreleg detached, specimen dissected; | PSD 489 | PSD 182 |MNRJ-ENT6-29222 ( MNRJ ), condition: both forelegs removed for DNA preservation, specimen dissected; (1) Expedição Solstício, Ponto 02 / R[io de]J[aneiro], Resende, P [arque]N[acional] do Itatiaia, / caminho do Abrigo Rebouças—/ 22 o 21′52.12″S , 44 o 43′24.88″W alt. 2450 m / 26.xii.2019 / AB Kury , MA Medrano & DR Pedroso leg. ( MNRJ ), condition: both forelegs detached, FWs detached; (2) Brasil , R[io de]J[aneiro], Itatiaia / P[arque]N[acional do] Itatiaia. Caminho do abrigo Rebouças / 22 o 21′52.12″S , 44 o 43′24.88″W — 2450 m / 26.xii.2019 / Kury, Medrano, Pedroso cols ( MNRJ ) ( MZSP ), condition: one foreleg missing, one foreleg detached, specimen dissected . Paratype females. (3) Same data as for allotype | PSD 154 | MNRJ-ENT6-29216 ( MNRJ ), condition: one foreleg removed for DNA preservation; PSD 183 |MNRJ-ENT6- 29215 ( MNRJ ), same condition as previous paratype ; PSD 186 | MNRJ-ENT6-29227 ( MNRJ ), same condition as previous paratypes ; (2) Same data as for holotype | PSD 487 | MNRJ-ENT6-29217 ( MNRJ ), condition: one foreleg removed for DNA preservation, one foreleg detached, specimen dissected; MNRJ-ENT6-29224 ( MZSP ), condition: one foreleg missing . Diagnosis . This species is distinguished from Bambuina bambui by the following characters: proximal part of males FWs folded, metanotal glandular structures absent. Male: FWs shorter than B. bambui , not surpassing the posterior margin of first abdominal tergite, without longitudinal vein; PsP1 inner margin short, outer margin elongated, pointed; PsP2 less developed. Female: copulatory papilla rounded, proximal margin flattened, with central, distal protuberance rounded. FIGURE 1. Bambuina zikani n. sp. Living specimens: A, B and D, male; C, female. Description . Male . General body coloration in several shades of brown, marbled ( Figs. 1 , 2 ). Head . Head elongated, in lateral view ( Fig. 2E ). Dorsum pubescent, light to medium brown, marbled ( Fig. 2B ). Occiput and vertex light to medium brown ( Figs 2B–D ). Fastigium medium brown, longer than wide, narrower than scape, separated from vertex by thin line yellowish brown. Three ocelli present, rounded, central flattened at bottom ( Fig. 2A ); eyes obovate ( Figs. 2C–E ). Antennal scape inner half medium brown, outer half light brown ( Figs. 2A, C–E ). Antenna not annulated; antennomeres medium brown, uniform ( Fig. 1 ). Frons medium brown, with two maculae light brown below antennal scapes, four spot circular, light brown, on inferior part, two horizontal lines light brown below eyes ( Fig. 2A ). Frontoclypeal suture yellowish brown, with two dark brown spots ( Fig. 2A ). Clypeus and labrum light brown; mandibles medium brown ( Figs. 2A, E ). Maxillary palpi elongated, thin, medium to light brown, articles 3, 4, and 5 almost same-sized, apex of article 5 slightly upcurved, whitish ventrally ( Figs. 2A, E ). In lateral view, gena yellowish brown from the eye margin to posterior border, medium brown on inferior portion, with an ascendant line medium brown on posterior border ( Figs. 2C, E ). FIGURE 2 . Bambuina zikani n. sp. A, male, head, frontal; B, male, head and thorax, dorsal; C, male, head and thorax, lateral; D, head and thorax, lateral (showing secretions of FWs); E, male, head, lateral; F, male, metanotum, dorsal; G, female, FW (red arrow); H, male, supra anal plate; I, male, subgenital plate; J, female, supra anal plate; K, female, subgenital plate. Scales: 1mm. FIGURE 3 . Bambuina zikani n. sp. Male and female genitalia. Male genitalia: A, dorsal; B, ventral; C, lateral; D, posterior. Female genitalia: E, dorsal; F, dorsolateral; G, lateral Scales, 1mm. Abbreviations see Material and Methods. Thorax . Pronotum DD wider than long, slightly pubescent, light to dark brown, marbled, median portion with two large blotches light brown ( Fig. 2B ); inflated, divided by sagittal line light brown; cephalic margin almost straight, caudal margin slightly convex ( Fig. 2B ); LL medium brown, ventro-cephalic angle rounded, ventro-caudal angle oblique, ventral margin gradually ascendant ( Figs. 2C, D ). Legs . FI and II medium brown, annulated with light brown ( Fig. 1 ). TI and II medium brown annulated with light brown ( Fig. 1 ). TI with two same-sized apical spurs, TII with two same-sized apical ventral spurs. FIII light brown, three bands of several stripes medium brown on outer surface, apical third dark brown, annulated with light brown ( Fig. 1 ). TIII medium brown, annulated with light brown. subapical spurs 4/4, the first near apical spurs; no spines between subapical spurs 1 and 2; 4-5 spines between subapical spurs 2 and 3, 3 and 4; serrulation above subapical spurs. Apical spurs more developed on inner side; inner apical spurs: median one longer (iam), dorsal almost same sized (iad), ventral smallest (iav) (iam>iad>iav); outer apical spurs: median one longer (oam), dorsal (oad) little longer than ventral (oav) (oam>oad>oav). Basitarsi I, II and III anteriorly light brown, posteriorly medium brown, pubescent ( Fig. 1 ). Abdomen . light to medium brown, marbled ( Fig. 1 ). Supra anal plate light to medium brown, anterior margin concave, distal angles rounded, posterior margin straight ( Fig. 2H ). Subgenital plate medium brown, light brown centrally, anterior margin sub-straight, posterior margin rounded ( Fig. 2I ). Cerci medium brown. Male. FWs medium brown, coriaceous, pubescent, short, not triangular (in comparison with. B. bambui ) and not reaching posterior margin of first abdominal tergite ( Figs. 1A, B , 2B ); without developed veins ( Fig. 4A ); proximal part folded ( Figs. 4B, C ); apical part light brown, thickened, folded, glandular ( Figs. 2B–D , 4 ). Metanotal glandular projections absent ( Fig. 2F ). Male genitalia . Pseudepiphallus: basal portion of pseudepiphallic sclerite medially constricted ( Fig. 3A ); pseudepiphallic arms longer than ectophallic apodeme, apex larger than basis, curved inwards, covered with bristles ( Figs. 3A, C, D ). PsP1 broad, with two lobes: one internal, short, truncated; one external, elongated, pointed, visible in dorsal view ( Figs. 3A–C ). PsP2 shorter than PsP1, between pseudepiphallic sclerite and PsP1, visible in posterior view ( Figs. 3A, C, D ). Ectophallic invagination: EctAp well developed, robust, shorter than pseudepiphallic arm, slanted outwards ( Figs. 3A, B ); arc located posteriorly to the basal margin of pseudepiphallus; ventral projections of ectophallic invagination shorter than EctAp, robust ( Fig. 3B ); EctF membranous, surrounding endophallic sclerite. Endophallus: End crest shaped, EndAp present ( Figs. 3B, D ). FIGURE 4. Bambuina zikani n. sp. Male right FW. A, dorsal; B, ventral; C, lateral. Scales, 1mm. Female . Larger than male, coloration similar ( Fig. 1 ). Micropterous, FW very reduced ( Fig. 2G , red arrow). Supra anal plate pilose, light to medium brown, anterior margin slightly concave, posterior margin rounded ( Fig. 2J ); subgenital plate pilose, light to yellowish brown, anterior margin slightly concave, posterior margin centrally concave ( Fig. 2K ); ovipositor shorter than FIII. Female genitalia. Copulatory papilla rounded, proximal margin flattened, dark brown, with central protuberance rounded, distal, upper portion membranous ( Figs. 3E–G ). Remarks . Individual morphological variation was observed in the male genitalia of some specimens: the external lobe of PsP1 of the holotype is longer and the apex more acute than in paratypes . Measurements (mm). Males (n=6), mean (range): HW, 3.98 (3.86–4.1); IOD, 2.63 (2.3–2.8); PL, 2.9 (2.7–3.1); PW, 4.9 (4.7–5.2); FWL, 2.92 (2.4–3.4); FWW, 1.94 (1.6–2.1); LFIII, 17.72 (17–18.8); LTIII, 19.71 (19.2–21.1). Females (n=6), mean (range): HW, 4.43 (4.4–4.9); IOD, 2.85 (2.7–3.1); PL, 3.13 (2.8–3.3); PW, 5.55 (5.1–5.9); LFIII, 18.47 (17–19); LTIII, 20.32 (19.5–23); OL, 15.87 (14.7–23).