A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene rolamossa Iniesta & Ferreira, 2013 Figs 30D , 117–118 , 164I , 166H , 172A , 178K , 187 ; Supp. file 4: Figs 217C , 219B Pseudonannolene rolamossa Iniesta & Ferreira, 2013c: 77 , figs 2a–c. Pseudonannolene rolamossa – Gallo & Bichuette 2019: 48 . Diagnosis Males of P. rolamossa differ from those of all other species of the genus by having a solenomere with a subtriangular and elongated ectal process exceeding in length the rounded apicomesal process ( Fig. 118D ). Etymology Noun in apposition, taken from the State Park “Parque Estadual do Rola Moça” where the species was found ( Iniesta & Ferreira 2013c ). Material examined Holotype BRAZIL • ♂ ; Minas Gerais , Nova Lima , Rola Moça I cave ; [ -20.020857 , -43.812518 ]; 22 Mar. 2012 ; R.L. Ferreira and M. Souza-Silva leg.; ISLA 4004 . Paratypes (total: 1 ♀ ) BRAZIL • 1 ♀ ; same collection data as for holotype; ISLA 4005 . Other material (total: 7 ♂♂ , 2 ♀♀ , 4 immatures ) BRAZIL – Minas Gerais • 1 ♂ ; Brumadinho , Serrinha 02 cave ; [ -20.151476 , -44.201095 ]; 784 m a.s.l. ; M.P. Oliveira leg.; ISLA 15054 • 1 ♂ ; PBR_18 cave; 15–20 Mar. 2010 ; R. Bessi et al. leg.; IBSP 5903 • 1 ♂ , 2 immatures ; Nova Lima, TUTA-14 cave ; [ -19.993344 , -43.849412 ]; 763 m a.s.l. ; M.P. Oliveira leg.; ISLA 15038 • 1 ♂ ; Rio Acima , ABOB_0028 cave; [ -20.087775 , -43.790650 ]; 743 m a.s.l. ; 13 Jun. 2019 ; Equipe Spelayon leg.; IBSP 7766 • 1 immature ; same collection data as for preceding; IBSP 7767 • 2 immatures ; same collection data as for preceding; IBSP 7769 • 1 ♂ ; same collection data as for preceding; IBSP 7772 • 1 ♂ , 2 ♀♀ ; same collection data as for preceding; IBSP 7773 • 1 ♂ ; Mariana, GS_25 cave; [ -20.365015 , -43.414773 ]; 780 m a.s.l. ; 16 Jan.–11 Feb. 2011 ; Bessi et al. leg.; ISLA 6594 . Descriptive notes MEASUREMENTS . 60–62 body rings (1–2 apodous + telson). Males: body length 56 mm ; maximum midbody diameter 4 mm . Females: body length 58 mm ; maximum midbody diameter 4.1 mm . COLOR . Body color brownish grey; head, antennae, collum, and legs darker; prozonites anteriorly greyish; metazonites with a medial darker band and a posterior lighter one. HEAD . Antennae long ( Fig. 164I ), just reaching back to end of ring 6 when extended dorsally; antennomeres elongated; relative antennomere lengths 1<2<3>4>5≈6>7. Mandibular cardo with ventral margin narrow. Ommatidial cluster well-developed, elliptical; ca 38 ommatidia in 6 rows. BODY RINGS . Collum with lateral lobes rounded, with ca 10 striae, strongly curved ectad ( Fig. 117A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae above ozopore in anterior body rings. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 172A ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) short (less than half of remaining podomere lengths), subtriangular, with the base arched and expanded, densely setose ( Fig. 118A ); prefemoral process ( prf ) about as wide as half of prefemur, subcylindrical, densely setose up to its median region ( Fig. 118B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) large and rounded; penis ( pn ) located at proximal region, rounded, not extended basally ( Fig. 118C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) elongated, twice longer than telopodite, with the base slightly arched; antero-posteriorly flattened ( Fig. 118D–F ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder ( sh ) long, subtriangular. Telopodite ( tp ) almost as wide as gcx ( Figs 118D , 217C , 219B ); solenomere ( sl ) with apicomesal process ( amp ) short, rounded; ectal process ( ep ) subtriangular, elongated, exceeding in length the amp ; sa located at mesal portion, nearly not visible apically. Internal branch ( ib ) subtriangular, narrow, surrounding basally tp as a shield; with torsion of 180° in the distal portion but without projection; ib with setae along its entire margin slightly exceeding apically seminal region of sl ( Fig. 118D–F ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 178K ); internal valve subtriangular; operculum narrow; external valve wide, subtriangular. Distribution The species is widely distributed in iron ore caves and surrounding forests in the central region of Minas Gerais State , Brazil ( Fig. 187 ).