A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene buhrnheimi Schubart, 1960 Figs 28D , 53–55 , 163D , 165E , 167D , 177E , 181 ; Supp. file 4: Figs 200E , 202F , 214B , 217A , 219D Pseudonannolene buhrnheimi Schubart, 1960: 79 . Pseudonannolene buhrnheimi – Jeekel 2004: 88 . Diagnosis Pseudonannolene buhrnheimi resembles P. granulata sp. nov. by having metazonites granulated ( Fig. 53 ) and epiproct with subtriangular process ( Figs 53B , 54 ), but differs by the absence of proximal projections on the stipes ( Fig. 167D ). Etymology Patronym honoring the collector Paulo Bührnheim ( Schubart 1960 ). Material examined Holotype BRAZIL • ♂ [gonopods and first leg-pair on microscope slides]; Rio de Janeiro , Santa Teresa ; [ -22.942260 , -43.212212 ]; 16 Oct. 1960 ; O. Schubart , J. Schubart and P. Bührnheim leg.; MZSP . Paratypes (total: 6 ♂♂ , 3 ♀♀ , 2 immatures ) BRAZIL • 6 ♂♂ , 3 ♀♀ , 2 immatures ; same collection data as for holotype; MZSP . Other material (total: 4 ♂♂ , 7 ♀♀ , 3 immatures ) BRAZIL – Rio de Janeiro • 1 ♂ ; Cachoeiras do Macacu , Reserva Ecológica Guapiassú ; [ -22.452806 , -42.770293 ]; 34 m a.s.l. ; 8–12 Oct. 2001 ; Equipe Biota leg.; IBSP 2402 • 1 ♀ ; same collection data as for preceding; IBSP 2406 • 1 ♀ ; same collection data as for preceding; IBSP 2403 • 1 ♀ ; same collection data as for preceding; IBSP 2397 • 1 ♀ , 1 ♀ immature; same collection data as for preceding; IBSP 2404 ; • 1 ♂ , 1 ♀ ; same collection data as for preceding; IBSP 2385 • 1 ♂ , 1 ♀ immature; same collection data as for preceding; IBSP 2399 • 1 ♀ , 1 ♂ immature; same collection data as for preceding; IBSP 2384 • 1 ♂ , 1 ♀ ; Rio de Janeiro , Santa Teresa ; [ -22.942260 , -43.212212 ]; 16 Oct. 1960 ; O. Schubart , J. Schubart and P. Bührnheim leg.; MZSP . Descriptive notes MEASUREMENTS . 54–60 body rings (1–2 apodous + telson). Males: body length 55.9–69.4 mm ; maximum midbody diameter 3–3.9 mm . Females: body length 67.6–76.6 mm ; maximum midbody diameter 3.9– 4.8 mm . COLOR . Body color brownish; collum darker; prozonites greyish anteriorly; metazonites with a medial brown band and a reddish posterior band; antennae and legs lighter brown. HEAD . Antennae long ( Fig. 163D ), just reaching back to end of ring 6 when extended dorsally; antennomeres elongated; relative antennomere lengths 1<2<3>4>5=6>7. Mandibular cardo with ventral margin narrow. Ommatidial cluster well-developed, elliptical; ca 40 ommatidia in 5 rows. BODY RINGS . Collum with lateral lobes rounded, with ca 9 striae, slightly curved ectad ( Fig. 53A ). Well demarcated constriction between prozonite and metazonite ( Fig. 53B ); prozonites smooth; metazonites granulated and laterally with transverse striae above ozopore. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 167D ). Epiproct with a long triangular process ( Fig. 54 ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) short (less than half of remaining podomere lengths), subtriangular, with the base arched, densely setose mainly on distal region ( Fig. 55A ); prefemoral process ( prf ) short (less than half of prefemur), subcylindrical, densely setose up to its median region ( Fig. 55B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) large and rounded; penis ( pn ) located at proximal region, circle-shaped ( Fig. 55C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) elongated, almost twice as long as telopodite, with the base arched; slightly flattened antero-posteriorly ( Fig. 55D–F ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder ( sh ) rounded. Telopodite ( tp ) almost as wide as gcx ( Fig. 55D ); solenomere ( sl ) with apicomesal process ( amp ) subtriangular, larger; ectal process ( ep ) subtriangular, separating from amp by shallow notch; sa located at mesal portion, slightly visible apically. Internal branch ( ib ) subtriangular, narrow, slightly curved ectad at midlength, surrounding base of tp as a shield; ib with setae along its entire margin exceeding apically seminal region of sl ( Fig. 55D–F ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 177E ); internal valve subtriangular, with mesal region rounded; operculum narrow; external valve wide, subtriangular. Distribution Known from the Atlantic Forest in the southern and central regions of Rio de Janeiro State , Brazil ( Fig. 181 ).