Streptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges Author Li, Lei Author Wang, Jie Author Zhou, Yong-jun Author Lin, Hou-wen Author Lu, Yan-hua text International Journal of Systematic and Evolutionary Microbiology 2019 2019-01-01 69 1 99 104 http://dx.doi.org/10.1099/ijsem.0.003109 journal article 20676 10.1099/ijsem.0.003109 c3dc5f9c-9f76-4923-8268-e788461dedd5 1466-5034 6048642 DESCRIPTION OF STREPTOMYCES RENIOCHALINAE SP. NOV. Streptomyces reniochalinae (re.ni.o.cha′ li.nae. N.L. gen. n. reniochalinae of the marine sponge Reniochalina stalagmitis ). Aerobic, Gram-stain-positive, non-motile, non-acid–alcohol-fast actinomycete that forms extensively branched substrate mycelia and white aerial hyphae which differentiated into chains of smooth-surfaced spores. Growths occurs at 20–40 Ǫ C and at pH 6.0–11.0 with 0–15% NaCl. Gelatin, and Tweens 40 and 60 are degraded, but CMC, aesculin and Tween 80 are not. Degradation of Tween 20 and starch are weakly positive. Milk coagulation, peptonization and nitrate reduction are positive. Utilizes L- arabinose, D- arabinose, cellobiose, D- fructose, D- galactose, D- glucose, glycerol, lactose, maltose, D- mannitol, D- mannose, melibiose, L- rhamnose, D-ribose, trehalose, D- xylose, sodium acetate, sodium pyruvate and sodium citrate as sole carbon sources; weakly utilizes inositol and raffinose, but not dulcitol, D- sorbitol, sucrose or D- xylitol. Utilizes L- glutamine, L- lysine, L- proline, L- serine and L- threoninexanthine as sole nitrogen sources; weakly utilizes L- alanine, L- arginine, L- asparagine, glycine, L- histidine, hypoxanthine, L- hydroxyproline and L- leucine, but not adenine, L- aspartic acid, L- cystenine, L- glutamic acid, L-methionine, L- phenylalanine, L- tryptophan, L- tyrosine or L-valine. The cell wall contains LL- diaminopimelic acid and the whole-cell sugars are galactose, glucose and ribose. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol are the predominant polar lipids. The menaquinone system contains MK-9(H 6 ) as a major component, and MK-9(H 4 ) and MK-9(H 8 ) as minorities. The major fatty acids are iso-C 16: 0 , anteiso-C 15: 0 and anteiso-C 17: 0 . The G+C content is 71.9 mol% of the 7.6 Mb draft genome. Fig. 3. Neighbour-joining tree based on five-gene concatenated sequences ( atpD , gyrB , recA , rpoB and trpB , 2454 nt) showing the relationships between strains LHW50302 T , LHW51701 T and related members of the genus Streptomyces . Kitasatospora aburaviensis NRRL B-2218 T was used as an outgroup. Numbers at nodes indicate levels of bootstrap support (%) based on neighbour-joining analysis of 1000 resampled datasets; only values above 50 % are shown. Asterisks (*) and crosses (‡) indicate branches that were also found using the maximum-likelihood method and the maximum-parsimony method, respectively. Bar, 0.02 substitutions per site. The type strain, LHW50302 T (=DSM 106194 T =CCTCC AA 2018013 T ), was isolated from a Reniochalina stalagmitis marine sponge collected in the South China Sea. The GenBank accession number for the 16S rRNA gene sequence and the draft genome sequence of the type strain are KX347891 and QOIM00000000, respectively .