On the identity of Trichogramma demoraesi Nagaraja (Hymenoptera: Trichogrammatidae), with a checklist and a key to Trichogramma species associated with Erinnyis ello (L.) (Lepidoptera, Sphingidae) in Brazil Author Vieira, Jaci M. Departamento de Entomologia e Acarologia, Escola Superior de Agricultura Luiz de Queiroz, 13418 - 900 Piracicaba, São Paulo, Brazil. E-mail: jacimvieira @ yahoo. com. br; razucchi @ usp. br jacimvieira@yahoo.com.br Author Querino, Ranyse B. Empresa Brasileira de Pesquisa Agropecuária, Embrapa Meio-Norte, 64006 - 220 Teresina, Piauí, Brazil. Author Zucchi, Roberto A. Departamento de Entomologia e Acarologia, Escola Superior de Agricultura Luiz de Queiroz, 13418 - 900 Piracicaba, São Paulo, Brazil. E-mail: jacimvieira @ yahoo. com. br; razucchi @ usp. br jacimvieira@yahoo.com.br text Zootaxa 2014 2014-09-29 3869 1 83 89 journal article 5339 10.11646/zootaxa.3869.1.8 fb7791ce-b3d7-4169-ba8d-249672e855ca 1175-5326 4947175 465BD523-EF94-4C86-925C-F119B7402A67 Trichogramma marandobai Brun, Moraes & Soares, 1986 Trichogramma demoraesi : Zucchi et al . 1991 (checklist); Zucchi & Monteiro 1994: 52 (record); Zucchi & Monteiro 1997: 53 (catalog); Querino & Zucchi 2005: 51 (key); Ronchi-Teles & Querino 2005: 515 (record); Zucchi et al . 2010: 222 (catalog); Querino & Zucchi 2011: 38 (identification guide). FIGURE 2. Genital capsule (male). A, B. Trichogramma manicobai ; C, D. Trichogramma marandobai (A, C ventral view; B, D dorsal view, 40x). FIGURE 3. Genital capsule (male). A, B. Trichogramma atopovirilia ; C, D. Trichogramma pretiosum (A,C ventral view; B,D, dorsal view, 40x) Diagnosis. Genital capsule long; dorsal lamina tapering from apex to base, not extending beyond apex of volsellae; dorsal lamina with narrow posterior extension and rounded apex at same level as intervolsellar process; intervolsellar process long and stout; ventral carina not extending beyond midlength of genital capsule ( Fig. 2 C, D ). Antennae with flagelliform setae relatively short, tapering at apex ( Fig. 4 B ). Type material . Holotype and paratype were deposited in the ICB/UFMG collection. However, these types are no longer at UFMG (Prof. Pedro M. Linardi, pers. comm.). Examined material. BRAZIL , Minas Gerais : Janaúba, 9.iii.2010 15 ♂ (J.M. Vieira) ( ESALQ ). Espírito Santo : Itapemirim, 1.vii.1985 , 1 ♂ , ex Erinnyis ello eggs (identified as T. demoraesi ) ( ESALQ ). Minas Gerais : Lavras, 15.i.2001 , 1 ♂ , 20.xii.2000 , 1 ♂ , ex Erinnyis ello eggs (identified as T. demoraesi ) ( ESALQ ). Paraná : Londrina, 18.iii.1986 , 1 ♂ , ex Erinnyis ello eggs (identified as T. demoraesi ) ( ESALQ ). São Paulo : Atibaia (Experimental Station of the Biological Institute), 5 ♂ ; Piracicaba, 1.vii.1999 , 15 ♂ , ex Erinnyis ello eggs (identified as T. demoraesi ) ( ESALQ ). PERU , locality unknown, 1.v.1999 , 15 ♂ , ex Erinnyis ello eggs (identified as T. demoraesi ). Loreto : Estación Jenaro Herrera, 13.i.2011 , 10 ♂ , ex Erinnyis ello eggs ( ESALQ ). Distribution. Brazil : Amazonas, Minas Gerais , Espírito Santo , Paraná , and São Paulo ; Peru : Loreto . Associated plant. Manihot esculenta Crantz. Habitat. Farm. Host insect. Erinnyis ello (L., 1758). FIGURE 4. Antenna (male). A. Trichogramma manicobai ; B. Trichogramma marandobai ; C. Trichogramma atopovirilia ; D. Trichogramma pretiosum (10x). TABLE 1. Morphological distinction between Trichogramma demoraesi and T. marandobai .

Characters	T. demoraesi (paratype)	T. marandobai (ESALQ collection)
Flagelliform setae	Long (0.08 mm)	Short (0.05 mm)
Flagelliform setae length/width	2.05	1.4
Basiconic sensillae	1-1-2-0-1-1	1-2-2-0(1)-1-1
Ventral carina length/basal distance	0.39	0.39
Dorsal opening	Very deep	Not deep
Dorsal opening length/dorsal lamina length	2.18	1.43
Ventral processes	Close to the intervolsellar process base	Close to the intervolsellar process base
Length of the posterior row of the fore wing	Half of the wing length (9 setae)	Half of the wing length (6–11 setae)
Intervolsellar process	Narrow/slender	Robust
Width of the posterior extension of the dorsal lamina at base	Narrow (18 µm)	Wide (34 µm)

Comments. The comparative morphological analysis of the paratype and the specimens previously identified as T. demoraesi from eggs of E. ello in cassava, revealed that these specimens belong to T. marandobai ( Table 1 ). Trichogramma demoraesi differs from T. marandobai by having longer flagelliform setae, narrower dorsal lamina at the base of the posterior extension (fork-shaped), a deeper dorsal opening, and a long stout intervolsellar process with the apex truncated or pointed ( Table 1 ). Therefore, T. demoraesi is associated only with G. bipennaria in forest habitats in Brazil . Trichogramma marandobai has previously been recorded as a parasitoid of E. ello eggs.