Two new species of Zhuliangomyces (Amanitaceae) from Hainan Island, China Author Huang, Ting 0000-0001-6171-3803 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China & 1668947367 @ qq. com; https: // orcid. org / 0000 - 0001 - 6171 - 3803 1668947367@qq.com Author Zhang, Wen-Hao 0000-0002-8983-7346 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China & 1163545854 @ qq. com; https: // orcid. org / 0000 - 0002 - 8983 - 7346 1163545854@qq.com Author Huang, Hong-Yan 0000-0003-4028-3124 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China & 932235661 @ qq. com; https: // orcid. org / 0000 - 0003 - 4028 - 3124 932235661@qq.com Author Gu, Yan-Ming 0000-0001-8796-0925 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China & 1569130659 @ qq. com; https: // orcid. org / 0000 - 0001 - 8796 - 0925 1569130659@qq.com Author Tang, Li-Ping 0000-0002-6905-3157 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, China & lipingtang 11 @ qq. com; https: // orcid. org / 0000 - 0002 - 6905 - 3157 lipingtang11@qq.com text Phytotaxa 2022 2022-12-06 575 1 57 67 http://dx.doi.org/10.1094/PDIS-04-22-0755-PDN journal article 203363 10.11646/phytotaxa.575.1.3 fb752bfc-f5bf-4065-b7f4-a45f90e8aa24 1179-3163 7403206 Zhuliangomyces terrus T. Huang & L.P. Tang , sp. nov. ( Figs. 3 , 5 & 6c–d ). MycoBank:—MB 839039. Diagnosis:— Zhuliangomyces terrus is similar to Z. ochraceoluteus but differing in its chocolate or dark brown pileus when young, non-striate margin, mostly broadly ellipsoid basidiospores (4–5 × 3–4.5 μm, Q m = 1.24), and fruiting in soil. Holotype :— CHINA . HAINAN PROVINCE : Ledong Li Autonomous County , Jianfengling National Forest Park , solitary or scattered on soil in a tropical broad-leaved forest, 18°44’16”N , 108°50’11”E , elev. 810 m , 10 th August 2020 , M. Mu 647 ( MHKMU M. Mu 647 ！ ). Etymology:—“ terrus ” refers to the fact that the species fruits on soil. Gene sequences ex-holotype:— MW732472 (ITS) and MW732413 (LSU). Description:— Basidiomata small-sized. Pileus 40 mm diam., hemispherical at first, soon becoming convex, then plano-convex or plane, often with a relatively low, broad umbo, chocolate or deep coffee or dark brown (5F7–5F8), particularly obvious when young, pale toward margin, pale brown to brown (5B5–5B6); surface smooth, strongly viscid; margin non-striate; context thin, white (1A1), unchanging. Lamellae free, up to 2 mm in width, white to cream (1A1), close to crowded, L = 150–160, l = 1–2. Stipe 60–65 × 4–5 mm , central, cylindrical, slightly attenuate upwards, light brown to brown (5B5–5B6), glabrous, viscid; context white, unchanging, soft to partially hollow in the center; stipe base subcylindrical. Annulus apical, brown (5B5–5B6), strongly viscid. Odor indistinct. Taste not recorded. FIGURE 5. Microscopic features of Zhuliangomyces terrus (MHKMU M. Mu 647, holotype). a . Basidia; b . Basidiospores; c . Pileipellis; d . Stipe trama. Bars: a = 6 μm, b = 2 μm, c = 15 μm, d = 20 μm. Basidiospores [80/4/2] (3) 4.0–5.0 × 3.0–4.5 (5) μm, Q = 1.15–1.28, Q m = 1.24 ± 0.03 (under SEM), predominantly broadly ellipsoid, inamyloid, colorless, hyaline, thin-walled, smooth under the light microscope, but verruculose under SEM. Basidia 22–30 × 5–8 μm, clavate, 4-spored; sterigmata 3–5 μm long. Cystidia absent. Lamella trama bilateral. Mediostratum 20–50 μm wide, composed of abundant, ellipsoid to clavate inflated cells 30–100 × 10–19 μm; filamentous hyphae 2–9 μm diam., abundant; vascular hyphae rare. Lateral stratum composed of abundant, ellipsoid to clavate inflated cells 35–80 × 11–20 μm; filamentous hyphae 5–13 μm diam. Subhymenium 25–50 μm thick, with 2–3 layers of subglobose, ovoid to ellipsoid or irregular cells 10–20 × 5–15 μm. Pileipellis 100–140 μm thick, an ixotrichoderm composed of vertically arranged terminal cells, 35–70 × 2–5 μm, cylindrical to narrowly clavate. Stipe trama composed of longitudinally arranged terminal cells 72–170 × 16–54 μm, slightly thick-walled, ellipsoid to very narrowly clavate; filamentous hyphae 4–13 μm diam., slightly thick-walled; vascular hyphae rare. Clamp connections abundant, present in all parts of basidiomata. Habitat :— Solitary or scattered on ground in a tropical broad-leaved forest; fruiting in August. Distribution:— Hainan Province , China. Additional specimen examined:— CHINA . HAINAN PROVINCE , Ledong Li Autonomous County , Jianfengling National Forest Park , solitary or scattered on soil in a tropical broad-leaved forest, 18°44′33″N , 108°50′32″E , elev. 1000 m , 10 th August 2020 , T . Huang 345 ( MHKMU T . Huang 345) .