A review of Neotropical species in Thienemanniella Kieffer (Diptera, Chironomidae) Author Wiedenbrug, Sofia Author Lamas, Carlos E. Author Trivinho-Strixino, Susana text Zootaxa 2013 3670 2 215 237 journal article 10.11646/zootaxa.3670.2.7 ca02f198-cd57-4096-ad51-bc11ca524d95 1175-5326 215940 A83EF888-DBFF-4320-92D7-252A8DE34E85 Thienemanniella ubatuba sp. n. ( Figs 9–11 ) Type material. Holotype male with pupal and larval exuviae; BRAZIL , SP, Ubatuba , Sertão da Quina, Cachoeira da Renata, bridge, 23°31.231´S , 45°14.625´W , 33 m a.s.l., 24.I.2011 , S. Wiedenbrug. Paratypes : allotype female with pupal and larval exuviae; same data as holotype , except 8.I.2011 , UFSCar SW070. Two females with pupal and larval exuviae; same data as holotype , except one of them UFSCar SW071. One pharate male and three females, all with pupal and larval exuviae, two of them UFSCar 0 66 and 0 68, and one pharate female, UFSCar SW065, all same data as holotype , except 21.I.2011 . One male with pupal and larval exuviae, UFSCar SW069, same data as holotype , except 29.XII.2010 . Additional material examined. Two males, two pharate males and one pharate female, all with pupal exuviae, and three pupal exuviae; PR, São Luis do Purunã, encontro das águas, 25°27.099´S , 48°43.459´W , 10.I.2010 , S. Wiedenbrug. Four pupal exuviae; MG, Gonçalves, 22°38´37.50´´S , 45°51´27.40´´W , waterfall “Cachoeira das Andorinhas”, 14.I.2009 , S. Wiedenbrug. Three pupal exuviae; MG, Paraisópolis, 22°39´54.81´´S , 45°55´38.28´´W , 2nd-order stream, 1.370 m a.s.l., 11.X.2009 , S. Wiedenbrug. Diagnostic characters. The pupa of T. ubatuba is distinguished from other members of the group by sternite I with very fine shagreen, tergite VIII with shagreen with elongate median and posterior spinules, male sternite VIII with median short spines and posterior spinules stronger but not wide-based. The male of T. ubatuba can be distinguished from T. liae Paggi by abdominal tergite color, TII and III being whitish in T. ubatuba , brown in T. liae . The absence of oral projections on the transverse sternapodeme can help to separate T. ubatuba and T. sanctivincenta from T. sancticaroli . The male of T. ubatuba cannot be distinguished from T. spreta . The larva is very similar to T. sancticaroli , but the antenna/postmentum length ratio about 1.0 is diagnostic for T. ubatuba . Male (n = 1–3). Total length 1.21–1.60 mm . Wing length 0.63–0.76 mm . Color. Thorax brownish. Tergite I whitish with brownish oral borders; TII and III whitish with brownish median area; TIV–IX brown; TVI and TVII with a white posterior rectangle. Head. AR = 0.33–0.38. Antenna with 12 flagellomeres, apical flagellomere 100–120 µm long. Flagellomeres with more than one row of setae each. Eyes hairy. FIGURE 9. Thienemanniella ubatuba sp. n. , adult male. A . Fore trochanter. B . Hind trochanter. C–D . Apex of hind tibia in posterior (C) and anterior view (D). E . Wing. F . Hypopygium, general view. G. Hypopygium with tergite IX and gonostyli removed, internal sclerites hatched; left: ventral view, right: dorsal view. H. Gonostylus. Thorax. Antepronotal lobes tapering, dorsally parallel-sized. Wing. Clavus/wing length ratio 0.33–0.37. Anal lobe present ( Fig. 9 E). Legs. Fore and hind trochanter with dorsal keel ( Fig. 9 A, B). Hind tibial scale 5 µm long, with one short spur and one long, curved seta ( Fig. 9 C, D). Tergites. TI with 2 setae; TIII–VII with 5 setae; TVIII with 2 setae. Hypopygium ( Fig. 9 F, H). Tergite IX with 6–8 setae. Laterosternite IX with 1 seta. Superior volsella elongated with posterior margin at about 45° angle to median margin. Inferior volsella rounded, positioned apically on gonocoxite margin. Transverse sternapodeme 30–32 µm long, without lateral projections; phallapodeme curved to posterior. Gonostylus with a low crista dorsalis. For measurements, see Table 1 . Female (n = 1–2). Wing length 0.57–0.67 mm . Head. AR = 0.40. Antenna with 5 flagellomeres, apical flagellomere 40 µm long. Flagellomeres with one row of setae each. Eyes hairy. Wing. Clavus/wing length ratio 0.54. Anal lobe present ( Fig. 10 A). 6 setae at clavus in addition to marginal wing setae (not drawn). FIGURE 10 . Thienemanniella ubatuba sp. n. , adult female. A . Wing. B–C . Genitalia. B. Ventral view, C. Dorsal view, also showing coxosternapodemes and labia. Legs. Hind tibial scale 7 µm long, with one short spur and one long and curved seta. Hind Ta1 with 4–6 sensilla chaetica. Tergites II–V with 5 setae, VI with 3 or 5, VII with 3, VIII without setae. Genitalia ( Fig. 10 B, C). Tergite IX with 7 setae. Laterosternite IX with 1 seta. Two subequal seminal capsules, 50 μm long; spermathecal ducts join shortly before seminal eminence. Notum 40 μm long. Apodeme lobe approximately lozenge-shaped, evenly sclerotized, brown. Coxosternapodeme curved. Copulatory bursa with oral median invagination and sclerotized oral borders. Labia membranous, bare. Gonocoxapodeme slightly curved. Cercus 22 μm long. For measurements, see Table 2 . Pupa (n = 2–3). Total length 1.45–1.80 mm . Cephalothorax. Frontal apotome rugose. Thorax suture slightly rugose. Dc-setae in two pairs; Dc1 and Dc3-4 long and taeniate, Dc2 short and hair-like. Wing sheath without pearl rows. Abdomen ( Fig. 11 A, B). Tergite I bare; TII–V with fine shagreen, with posterior spinules larger; TVI–VIII with homogeneous shagreen, with oral median spinules elongated. Sternite I with very fine shagreen; SII with elongated spinules; SIII–VIII with fine homogeneous shagreen. Tergal conjunctives III/IV–VII/VIII and sternal conjunctives III/IV–VII/VIII with small spinules. Segment I with 1 L-seta, II with 3 and III–VIII with 4 long taeniate L-setae. Anal lobe rounded, with almost complete fringe, 3 taeniate macrosetae, and 1 taeniate median seta. Male genital sac with inner median margin oblique. For measurements, see Table 3 . Larva (n = 4). Head. Postmentum 132–140 µm long. Head capsule integument yellow and smooth. Mentum ( Fig. 11 C) with three median teeth, central tooth much smaller than adjacent teeth; first lateral teeth adpressed to outer median teeth. Antenna 135–140 µm long ( Fig. 11 D); segments two and three darker than first segment. Abdomen. Hair-like setae not modified. Posterior parapod with subbasal seta simple. For measurements, see Table 4. Remarks. See remarks on T. sancticaroli sp. n.