Novel species of Cladosporium from environmental sources in Spain Author Iturrieta-Gonzalez, Isabel https://orcid.org/0000-0001-8426-9530 Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, 43201, Reus, Tarragona, Spain Author Garcia, Dania Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, 43201, Reus, Tarragona, Spain Author Gene, Josepa https://orcid.org/0000-0001-6195-9299 Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, 43201, Reus, Tarragona, Spain josepa.gene@urv.cat text MycoKeys 2021 2021-01-05 77 1 25 http://dx.doi.org/10.3897/mycokeys.77.60862 journal article http://dx.doi.org/10.3897/mycokeys.77.60862 1314-4049-77-1 F53D2641398253F988840BC0854594BB Cladosporium coprophilum Iturrieta-Gonzalez , Dania Garcia , Gene sp. nov. Fig. 4 Etymology. Name refers to the substrate where the species was isolated, unidentified herbivore dung (ancient Greek, kopros = dung + philos = loving). Type. Spain, Extremadura, Badajoz province, Granja de Torrehermosa, unidentified herbivore dung, Jan. 2017, J. Cano (holotype CBS H-24470; cultures ex-type FMR 16164, CBS 144919). Description. Mycelium in vitro superficial and immersed, composed of septate, branched, pale brown, smooth hyphae, 3-5 μm wide. Conidiophores macronematous, arising laterally or terminally from hyphae, erect to slightly flexuous, non-nodulose, septate, unbranched, up to 124 μm long, 3-4 μm wide, pale brown, smooth. Conidiogenous cells integrated, terminal, rarely intercalary, cylindrical, (7-)14-33 x (2-)3-4 μm , bearing up to 3 conidiogenous loci, slightly darkened and refractive. Ramoconidia 0(-1)-septate, subcylindrical to cylindrical, 9-19 x 3-5 μm [av. ( +/- SD) 12.3 ( +/- 2.8) x 3.9 ( +/- 0.54)], pale brown, smooth. Conidia forming branched chains, with up to five conidia in the terminal unbranched part, aseptate, pale brown, smooth to verruculose; small terminal conidia ellipsoidal to slightly obovoid, 4.5-7 x 2.5-4 μm [av. ( +/- SD) 6 ( +/- 0.64) x 3.1 ( +/- 0.31)]; intercalary conidia ellipsoidal, 6-10.5 x 2.5-4 μm [av. ( +/- SD) 7.7 ( +/- 1.32) x 3.3 ( +/- 0.37)]; secondary ramoconidia subcylindrical to cylindrical, 7-12.5 μm long x 3-5 μm [av. ( +/- SD) 9.6 ( +/- 1.7) x 4.2 ( +/- 0.51)]. Culture characteristics (14 d at 25 °C). Colonies on OA reaching 21-22 mm diam., olive (2F6) to black, dark green margin (30F4), flat, slightly dusty at the center, aerial mycelium scarce, margin regular; reverse dark green (30F8) to black. On PDA attaining 36-37 mm diam., olive (2F6/2E3), greenish gray margin, slightly depressed and irregularly folded at the center, velvety, aerial mycelium scarce, margin regular; reverse dark green (30F8/27F3). On SNA reaching 27-28 mm diam., olive (3F6/2F8), flat, slightly dusty, aerial mycelium scarce, margin regular; reverse dark green (30F8) to black. Cardinal temperature for growth. Optimum 20 °C, maximum 25 °C, minimum 5 °C. Distribution. Spain. Additional specimen examined. Spain, Extremadura, Badajoz province, Granja de Torrehermosa, unidentified herbivore dung, Mar. 2017, J. Cano (FMR 16101). Notes. Based on the multi-locus analysis (Fig. 1 ), C. coprophilum is allocated to a terminal low-supported clade together with C. chasmanthicola and C. sinuatum , species recently described from leaf spots of Chasmanthe aethiopica in South Africa ( Marin-Felix et al. 2017 ) and Alpine soil in China ( Ma et al. 2017 ), respectively. The new species is distinguished from C. chasmanthicola by the production of smooth hyphae (smooth to distinctly verrucose or irregularly rough-walled in C. chasmanthicola ), longer conidiogenous cells (up to 33 vs up to 24 μm ), shorter ramoconidia (9-19 vs 15-33 μm ) with fewer septa [(0(-1) vs 0-1(-3)-septate], and longer terminal conidia (4.5-7 vs 2.5-4.5 μm ) ( Marin-Felix et al. 2017 ). Cladosporium coprophilum differs from C. sinuatum by the production of aseptate intercalary conidia (0-1-septate in C. sinuatum ). In addition, C. sinuatum is characterized by distinctive geniculate-sinuous conidiophores and a rather fast growth on OA (40-45 mm vs 21-22 mm in C. coprophilum after 14 d at 25 °C) ( Ma et al. 2017 ). Figure 4. Cladosporium coprophilum (ex-type FMR 16164) a-c colonies on PDA, OA and SNA after 14 days at 25 °C d-e conidiophores f conidia. Scale bars: 10 mm ( a-c ); 10 μm ( d-f ).