Additions to Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) fungi: two new species and one new host record from China Author He, Yu-Ke https://orcid.org/0000-0003-3043-0493 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, 550025, China & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Yang, Qi Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, 550025, China Author Sun, Ya-Ru https://orcid.org/0000-0001-5549-1028 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Zeng, Xiang-Yu https://orcid.org/0000-0003-1341-1004 Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, 550025, China Author Jayawardena, Ruvishika S. https://orcid.org/0000-0001-7702-4885 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Hyde, Kevin D. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Wang, Yong Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, 550025, China yongwangbis@aliyun.com text Biodiversity Data Journal 2022 2022-09-28 10 90709 90709 http://dx.doi.org/10.3897/BDJ.10.e90709 journal article http://dx.doi.org/10.3897/BDJ.10.e90709 1314-2828-10-e90709 041DE2270BA253F597EF022F29F25211 Neopestalotiopsis samarangensis (Maharachch. & K.D. Hyde) Neopestalotiopsis samarangensis (Maharachch. & K.D. Hyde) Maharachch., K.D. Hyde & Crous in Maharachchikumbura, Hyde, Groenewald, Xu & Crous, Stud. Mycol. 79: 147 (2014) Materials Type status: Other material . Occurrence : recordedBy: Yu-ke He ; occurrenceID: GUCC 21003; Taxon : scientificName: Neopestalotiopsis samarangensis; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China ; stateProvince: Hainan ; locality: Haikou City , Xinglong Tropical Botanical Garden ; verbatimCoordinates: 110°11' E , 18°44' N ; Identification: identifiedBy: Yu-ke He ; dateIdentified: 2020; Record Level: collectionID: HGUP 10003 Description Associated with leaf spots of Salacca zalacca (Gaertn.) Voss. Disease symptom: a small oval scab, ring-like, the inner ring is light brown to dark brown and the outer ring is light brown, the boundary is obvious, dark brown. A few black, small, isolated and punctuate conidia irregularly distributed on the scab. Sexual state: unknown. Asexual morph (Fig. 4 ): Conidiomata is dark, oblate, scattered on the host scab, 70-180 μm . Conidiophores discrete to lageniform or globular, hyaline, smooth- and thin-walled, simple and short. Conidia 18-23 x 6-7.5 μm , fusiform to clavate, straight to slightly, 4-septate; basal cell obconic with a truncate base, hyaline or pale brown, smooth- and thin-walled, 3.5-5 μm long; three median cells 12.5-15 μm long, light brown or hyaline, septa and periclinal walls darker than rest of the cell, wall rugose; second cell 4.5-5.5 μm long; third cell 4-5.5 μm long; fourth cell 5-6 μm long; apical cell 3-4.5 μm long, hyaline, conic to acute, with 1-2 tubular appendages inserted at different loci, but in the same crest at the apex of the apical cell, unbranched, flexuous, 12-20 μm long. The spores have tubular appendages or single appendage, unbranched, centric, 3.5-6 μm long. Culture characteristics: Colonies on PDA medium reaching 4.5-5 cm diam. After 9 d at 24℃, odourless, without exudates, with black dots in the centre (conidiomata), the mycelium is white, soft and round with regular edges; reverse yellow to white. Under the surface of hyphal layer, releasing many conidia in a black, slimy mass. Notes Phylogenetically, isolated GUCC 21003 clustered with the ex-type strain of N. samarangensis (MFLUCC 12-0233). In morphology, our strain is very similar to N. samarangensis ( Maharachchikumbura et al. 2013 ). A comparison of DNA bases (Table 2 ) demonstrated that the differences between these two strains are minute. Therefore, we concluded that they are the same species, but occurring on different hosts ( N. samarangensis GUCC 21003 on leaf of Salacca zalacca vs. N. samarangensis MFLUCC 12-0233 on Syzygium samarangense ).