Madagascaromyces cannae sp. nov. (Mycosphaerellaceae) on Canna indica (Cannaceae) from Guangdong, China Author Chen, Jingwen 0000-0003-3048-1653 Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, P. R. China & Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, P. R. China & Jingwen 075 @ 163. com; https: // orcid. org / 0000 - 0003 - 3048 - 1653 Author Manawasinghe, Ishara S. 0000-0001-5730-3596 Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, P. R. China & Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, P. R. China & ishara 9017 @ gmail. com; https: // orcid. org / 0000 - 0001 - 5730 - 3596 ishara9017@gmail.com Author Lin, Jieying 0000-0003-0328-4523 Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, P. R. China & Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, P. R. China & linjieying 178 @ 163. com; https: // orcid. org / 0000 - 0003 - 0328 - 4523 Author Lin, Yanhong 0000-0001-6415-6467 Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, P. R. China & Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, P. R. China & yanhong 1624 @ 163. com; https: // orcid. org / 0000 - 0001 - 6415 - 6467 Author Wanasinghe, Dhanushka N. 0000-0003-1759-3933 Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe 654400, P. R. China & dnadeeshan @ gmail. com; https: // orcid. org / 0000 - 0003 - 1759 - 3933 dnadeeshan@gmail.com Author Zhang, Yunxia 0000-0002-2815-2554 Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, P. R. China & Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, P. R. China & yx _ zhang 08 @ 163. com; https: // orcid. org / 0000 - 0002 - 2815 - 2554 text Phytotaxa 2022 2022-09-06 561 1 53 64 http://dx.doi.org/10.11646/phytotaxa.561.1.5 journal article 10.11646/phytotaxa.561.1.5 1179-3163 7052843 Madagascaromyces cannae Y.X. Zhang, J.W. Chen & Manawas. sp. nov. ( FIGURE 2 ) Index Fungorµm number: IF 559623 Facesoffungi number: FoF 10814 Etymology: Epithet refers to the host genus from which the fungus was isolated Holotype : ZHKU 220029 FIGURE 2. Madagascaromyces cannae ( ZHKU 220029 Holotype ): a, b. Specimen observed. c, d. Colonies on MEA at 25 °C (b from the bottom). e. Spermatogonium forming on OA. f. Spermatia. g, h. Conidiophores. i, j. Conidiogenous cells. k–p. Conidia. Scale bars = 10 µm. Associated with leaf spot of Canna indica . Sexual morph : not observed. Asexual morph : Conidiophores (18–)20– 40(–78) × 2–4(–5) µm ( x = 31 × 3 µm, n = 50), 0–4 septate, pale brown. Conidiogenous cells (4–)7–15(–20) × (2–)4–5 µm ( x = 10 × 4 µm, n = 50), pale brown, terminal and lateral. Conidia (17–)30–50(–70) × 3–5 µm (n = 50), pale brown, clavate, guttulate, apex subobtuse, base long obconically subtruncate, 0–3(–6) septa (5 septa not observed), the septa are slightly constricted, some are slightly curved. Spermatogonia forming on OA, 510–770 × 540–770 µm ( x = 648 × 653 µm, n = 20), black dots, semi-immersed, globose. Spermatia 2.5–4.5 × 1.5–2 µm ( x = 3 × 2 µm, n = 50), smooth, nearly cylindrical. Cultural characteristics : Colonies on MEA reaching 27 mm diam after one month, flourishes aerial mycelium and smooth, surface white-grey; reverse dark green. Colonies on OA reaching 25 mm diam after one month at 25 ˚C, spreading with moderate aerial mycelium and smooth, surface Brown, reverse olivaceous-grey. Material examined : CHINA , Guangdong Province , Guangzhou , Haizhu National Wetland Park , on fresh leaves of Canna indica , 4 October 2021 , Jieying Lin , (dried culture ZHKU 220029 holotype ; ZHKU 220030 , ZHKU 220031 paratype ), living cultures ZHKUCC 220038, ex-type; ZHKUCC 220039, ZHKUCC 220040 ex-paratype . Notes : Three isolates obtained in the present study clustered with Madagascaromyces species in the phylogenetic analysis ( FIGURE 1 ). Our isolate differs from Madagascaromyces intermedius by the size of the conidia and the number of septa ( TABLE 3 ). Madagascaromyces intermedius develop conidia with 1–8 septa, while our isolate Madagascaromyces cannae has 0–6 septa (5 septa not observed), mostly 0–3 septa. Conidia sizes of our isolate (22–45 × 4 µm) are shorter and wider than Madagascaromyces intermedius [(35–)50–75(–100) × (2.5–)3 µm]. Based on a polyphasic approach, M . intermedius is identified as a new species.