Three new species and one subspecies of the Amynthas corticis - group from Guangxi Zhuang Autonomous Region, China (Oligochaeta, Megascolecidae) Author Dong, Yan Author Law, Michelle Man Suet Author Jiang, Jibao B. Author Qiu, Jiangping P. text ZooKeys 2019 884 23 42 http://dx.doi.org/10.3897/zookeys.884.30988 journal article http://dx.doi.org/10.3897/zookeys.884.30988 1313-2970-884-23 F1B4B6A8288C4CE9AFEF7DE08DBE9CC0 6251848C807E5E0896A9C39CC22ECD19 Amynthas maximus Qiu & Dong sp. nov. Figure 2 , Table 2 Material. Holotype :1 clitellate (C-GX201304-01A): China, Guangxi Zhuang Autonomous Region, Shiwan Mountain Nature Reserve ( 21.50299°N , 107.3035°E ), 449 m asl, black sandy soil under bryophytes in a subtropical evergreen forest, 12 May 2013, JP Qiu, Y Hong, JB Jiang, LL Zhang, Y Dong legit. Paratypes : 8 clitellates (C-GX201304-01B): same date as for holotype. Diagnosis. Dimensions 145-170 mm by 5.8-6.2 mm at clitellum, clitellum taupe in 2/5 XIV-XVI , 78-101 segments. First dorsal pore in 13/14. Setae numbering 33-38 at III, 32-36 at V, 29-33 at VIII, 18-22 at XX, 50-65 at XXV; 9-13 between male pores; setae between spermathecal pores numbering 14-16 at VI, 10-14 at VII, and 18-22 at VIII. Four pairs of spermathecal pores ventrally in 5/6-8/9. Seven or eight (three specimens have seven papillae, and four specimens have eight papillae) postsetal genital papillae arranged in two rows in VI-IX , 0.33 circumference ventrally apart from each other. One pair of male pores in XVIII, each on the top of a central, round porophore surrounded by three or four circular ridges, with one presetal indented-topped genital papilla medial of each porophore. Ampulla elongate oval, stout duct as long as 3/5 ampulla. Diverticulum slightly shorter than main pouch, a little twist in the middle, terminal 2/5 dilated into a swollen, club-shaped seminal chamber. Prostate glands well developed. Figure 2. A Ventral view showing spermathecal pores, female pores and male pores of Amynthas maximus sp. nov. B spermathecae of Amynthas maximus sp. nov. C illustration of the details of the male pore region. Table 2. A comparison of characters of A. maximus sp. nov., A. dorsualis , 2013, A. carnosus , A. corticis and A. wulinensis .

Characteristics	A. maximus sp. nov.	A. dorsualis	A. carnosus	A. corticis	A. wulinensis
Body length (mm)	145-170	121-?	110-340	45-170	128-174
Body width (mm)	5.8-6.2	2.7-?	4.0-9.0	3.0-6.0	5.6-6.1
Pigment	-	-	-	-	-
dorsum	Light purple brown before clitellum, from light purple brown to brown after	Dark grey before clitellum, dark brown after	Dark brown or purple	Greenish brown	Whitish purple
ventrum	No pigment before clitellum, yellowish after	Light grey before VII, no pigment after	Dark brown or purple	No	Whitish gray
First dorsal pore	13/14	13/14	12/13	10/11 or 12/13, usually at 11/12	11/12
Clitellum locality	2/5 XIV-XVI	1/10XIV-7/10XVI	XIV-XVI	XIV-XVI , occasionally shorter	XIV-XVI
Spermathecal pores	4 pairs, in 5/6-8/9, 0.33C	4 pairs, in 5/6-8/9, dorsally, 0.6C	4 pairs in 5/6-8/9 or 3 pairs in 6/7-8/9, 0.33C	4 pairs, 5/6-8/9, 0.33C	4 pairs, 5/6-8/9, ventral, 0.29C
Male pores	Middle, round, surrounded by 3-4 circular ridges, 0.4C	Slightly raised, glandular, surrounded by 5-6 elliptic circular folds, 0.33C	Round or elliptic	Small, circular to transverse elliptical disc, 0.24-0.30C	Round or oval-shaped on setal line with depressed center, 2-3 circular folds, 0.24-0.28C
Papillae preclitellum	7-8 post-setal indented-topped genital papillae arranged in two rows in VI-IX	Invisible	8 papillae just overhead 8 spermathecal pores, 2 pairs preclitellar arranged on VIII and IX	Paired presetal and/or postsetal in some or all, near spermathecal pores	Absent
Papillae postclitellum	2 presetal indented-topped genital papillae medial of porophore	Invisible	2 paired presetal genital on XVIII and IX, 1 pair postsetal genital on XVIII	Present or absent, occasionally one or more near male pore	Oval-shaped, medial to male pore in each of XVII and XIX, occasionally XX
Prostate glands	XVII-XIX	XVI-XX	Well developed	XVII-XX , rudimentary or absent	XV-XX , racemose, follicular
Spermathecae	About 1.6 mm long, ampulla long-oval; duct as long as 3/5 ampulla	About 2.2 mm long, ampulla heart-shaped; duct as long as 2/5 ampulla	Ampulla oval or pear-shaped, duct equal to or slightly shorter than ampulla	Ampulla ovoid	Very short and stout stalk
Diverticulum	Shorter, lightly twist in middle, terminal 2/5, swollen, club-shaped seminal chamber	Shorter, terminal 1/5, ovoid plump seminal chamber	One-third to half of ampulla, slender stalk and a wider seminal chamber	Blunt ovioid, straight stalk	Oval, shining white seminal chamber, a slender and straight stalk
Accessory glands	1 or 2 stalked accessory glands observed near ventral median line in VI, VII, VIII, IX	Invisible	-	Stalked, coelomic, bound down to parietes or retained within body wall	Paired in XVII and XIX, sessile, flowery

Description. External characters : Light purple-brown pigment on pre-clitellum dorsum, no pigment on ventrum. Pigment from light purple-brown to brown on post-clitellum dorsum, light yellowish on ventrum. Clitellum taupe in 2/5 XIV-XVI . Dimensions 160 mm by 6.0 mm at clitellum, 92 segments. Prostomium 1/2 epilobous. First dorsal pore in 13/14. Setae numbering 36 at III, 34 at V, 31 at VIII, 20 at XX, 60 at XXV; 12 between male pores; Setae numbering 15 at VI, 12 at VII, 20 at VIII between spermathecal pores. Setae formula: AA = 1.1-1.4AB, ZZ = 1.2-2.0ZY. Clitellum annular, yellowish, in 2/5 XIV-XVI , setae not visible externally. Four pairs of spermathecal pores in 5/6-8/9, ventral, eye-like, 0.4 circumference ventrally apart from each other. Seven or eight (three specimens have seven papillae, and four specimens have eight papillae) postsetal genital papillae arranged in two rows in VI-X , 0.33 circumference ventrally apart from each other. One pair of male pores in XVIII, 0.4 circumference apart ventrally, each on the top of a central, round porophore surrounded by three or four circular ridges, with one presetal indented-topped genital papilla medial of each porophore ( Figure 1A ). Single female pore in XIV, ovoid. Internal characters . Septa 5/6-7/8, 10/11-13/14 thick and muscular, 8/9-9/10 absent. Gizzard bucket-shaped, in VIII-X . Intestine enlarged distinctly from XV onwards. Intestinal caeca paired in XXVII, extending anteriorly to XXII, transition state, ventral margin smooth, four pointed saccules in dorsal margin. Four pairs of esophageal hearts in X-XIII , developed. Ovaries in XIII. Four pairs of spermathecae in VI-IX , short, approx. 1.6 mm long, ampulla elongate-oval; duct as long as 3/5 ampulla. Diverticulum slightly shorter than main pouch (ampulla and duct), a little twist in middle, terminal 2/5 dilated into a swollen, club-shaped seminal chamber ( Figure 1B ). One or two stalked accessory glands observed near ventral median line in VI-IX . Holandric: two pairs of testis sacs in X and XI, separated from each other, well developed. Two pairs of seminal vesicles in XI and XII, developed. Prostate glands undeveloped, inserting in XVIII and extending from XVII-XIX , coarsely lobate, prostatic duct I-shaped, of uniform thickness. No accessory glands observed in male pore region. Etymology. The species is named after its large accessory glands observed in the spermathecal area. Remarks. Amynthas maximus sp. nov. keys to the corticis -group in Sims and Easton (1972) with four pairs of spermathecal pores intersegmentally in 5/6-8/9. Amynthas maximus sp. nov. is similar to Amynthas carnosus (Goto & Hatai, 1899) as re-described by Chang et al. (2016) with respect to body size, the distance between spermathecal pores and male pores, shorter diverticulum than main spermathecal axis, and no accessary glands near prostates. In contrast, the pigment on its ventrum is lighter than A. carnosus and other differences include the first dorsal pore, clitellum location, spermathecal pores, and male pores characters. The first dorsal pore in A. maximus sp. nov. is located in 13/14, versus 12/13 in A. carnosus ; the clitellum occupies less than three segments; four pairs of spermathecal pores while sometimes three pairs in A. carnosus ; the porophore is surrounded by three or four circular ridges, but no ridges are present in A. carnosus ; several accessory glands observed in the spermathecal region in the new species but none in A. carnosus . We also compare the new species with Amynthas corticis (Kinberg, 1867) which has been recognized as the typical species in the corticis -group. They share several common characters such as body size, pigment, clitellum extent, setal number, and both have stalked accessory glands. Other than that, the first dorsal pore in the new species is in 13/14, but in 10/11 or 12/13, usually in 11/12 in A. corticis . The diverticulum of A. maximus sp. nov. has a small twist in the middle compared with A. corticis , which has a long stalk. Moreover, A. maximus sp. nov. always exhibits genital markings in the male pore region, whereas in A. corticis , these markings are occasionally absent. We further compare the new species with another species Amynthas dorsualis Sun & Qiu, 2013 described from Hainan, China, and its clitellum also occupies fewer than three segments. In our results, we find the two species share several common characters, including the first dorsal pore location, setal formula, male pore characters, and in the diverticulum being shorter than the main pouch. However, the morphological dissimilarity of the two species is substantial. For instance, the locations of spermathecal pores are different between A. dorsualis and A. maximus sp. nov. In A. dorsualis , the spermathecal pores are located on the dorsum, while the pores are located on the ventrum in A. maximus sp. nov. The distance between male pore is shorter on the ventral side in A. dorsualis than Amynthas maximus sp. nov. In addition, A. dorsualis has no genital markings and no accessory glands, whereas A. maximus sp. nov. exhibits genital markings near the spermathecal pores and the male pores region, and stalked accessory glands are present in spermathecal pores region. The body size of A. maximus sp. nov. is similar to A. wulinensis described from Taiwan Island. But the other characters of A. maximus sp. nov. differ from A. wulinensis . Amynthas maximus sp. nov. has no pigment before clitellum, yellowish after clitellum on dorsum, first dorsal pore in 13/14, 0.33C between spermathecal pores, seven or eight indented-topped genital papillae in VI-IX and accessory glands in spermathecal pores region. In contrast, A. wulinensis has whitish gray on dorsum, first dorsal pore in 11/12, 0.29C between spermathecal pores, no genital papillae observed in spermathecal pores region, and accessory glands observed in male pores region. Table 2 shows the comparison of characters of A. maximus sp. nov. with A. dorsualis , A. carnosus , A. corticis and A. wulinensis .