Contributions to the knowledge of predaceous midges of the subgenus Eukraiohelea Ingram & Macfie of Stilobezzia Kieffer (Diptera: Ceratopogonidae), from Brazil Author Bauer, Maria Luiza Felippe text Zootaxa 2017 2017-09-28 4324 3 557 570 journal article 31930 10.11646/zootaxa.4324.3.9 b9d558ba-c967-4d3f-96fc-0714d2e9869b 1175-5326 998492 Af52E5F5-6C6A-4382-89C9-E2A71B7Db4Db Stilobezzia ( Eukraiohelea ) proxima Cazorla & Felippe-Bauer n. sp. ( Figs. 15 ¯28) Diagnosis. The only Neotropical species of Stilobezzia ( Eukraiohelea ) of large size (wing length 1.50 – 1.82 mm ) with pale yellowish coloration and the lateral margins of abdominal tergites with dark brown stripes. Male with stout gonocoxites; gonostyli short, swollen at mid portion with curved apex and pointed tip; apicolateral processes short, rounded and parameres with short, ventrally directed tip. Female with tarsomere 5 of fore and mid legs with 2 pairs of batonnets, tarsomere 5 of hind leg with 1 pair of batonnets and two ovoid spermathecae measuring 87–90 µ. Male ( Fig. 15 ). Head ( Fig. 16 ) dark brown. Antenna with flagellomeres 1–10 pale yellowish basally, light brown distally, flagellomeres 11–13 dark brown with light brown basal ring; flagellomere 13 2.5X longer than flagellomere 12; plume brown, poorly developed; antennal ratio 1.30. Palpus ( Fig. 17 ) with segments 3–5 dark brown, 1–2 light brown; third segment elongate with subapical sensory pit; palpal ratio 3.50 (n = 2). Thorax. Scutum brown (as Fig. 15 , female), two faint yellowish lateral vittae extending posteriorly from paler humeral pits; scutellum pale yellowish except narrow lateral margins brown with 4 stout setae, 2–3 thinner setae; postscutellum brown. Legs pale yellowish; basal 1/3 of mid tibia slightly darker; narrow apex of hind femur dark brown; basal 1/3, narrow apex of hind tibia dark brown; fore femur with 2–3 short stout ventral spines; tarsomere 1 of mid leg with slender, straight basal spine; tarsomere 1 of hind leg with stout, curved basal spine; hind tibial comb with 6 spines; fore leg tarsal ratio 2.41–2.45 (2.43, n = 2); mid leg tarsal ratio 2.78–2.90 (2.79, n = 2); hind leg tarsal ratio 2.69–2.88 (2.79, n = 2); tarsomere 5 of all legs with 1 pair of batonnets ( Fig. 18 ) and large claws with long basal tooth. Wing ( Fig. 19 ) membrane hyaline; r-m crossvein oblique; cubital fork distal to level of base of rm crossvein; wing length 1.50–1.63 (1.57, n = 2) mm, width 0.50 (n = 2) mm; costal ratio 0.72–0.75 (0.73, n= 2). Halter stem dark brown; basal half of knob dark brown, distal half pale. Abdomen. Pale yellowish, lateral margins of tergites brown. Genitalia ( Figs. 20 ¯22): tergite 9 extending slightly beyond apex of gonocoxites, distal margin rounded with small mesal notch, apicolateral processes short with rounded apices, located laterad to the slender cerci ( Fig. 22 ); sternite 9 2.50 X broader than long, posterior margin not clearly distinguishable, apparently straight. Gonocoxite stout, 2.0 X longer than greatest breadth, each with a hyaline membrane extending from the dorsal inner margin to nearly 2/3 of the length of parameres, lateral broad margins of the membrane sclerotized, folded, connected dorsally by a broad, sclerotized transversal bridge; gonostylus pale yellowish, 0.60 X the length of gonocoxite, swollen at mid portion, tip curved, pointed. Parameres separate, parallel; gonocoxal apodemes moderately sclerotized, broad, fused to dorsum of basal arms of parameres; basal arms stout, blade-like, apices rounded, divergent; distal portions slender, rod-like, closely approximated except on apices, which gradually taper to slightly broader ventrally recurved, pointed tips. Aedeagus represented by 2 arched sclerites; basal portion heavily sclerotized, recurved; subapical portion slightly swollen, more lightly sclerotized; distal portion pointed, tips overlapping. Female. Similar to male, with the following notable sexual differences: Head ( Fig. 23 ). Antenna with flagellomeres 1–8 pale yellowish basally, pale brown distally, flagellomeres 9–13 dark brown with pale basal ring; antennal ratio 1.73–1.92 (1.82, n = 2). Palpal ratio 3.50–3.75 (3.60, n = 2). Mandible with 7 – 8 teeth. Thorax. Legs ( Fig. 24 ): fore femur with 2 – 4 short, stout ventral spines; tarsomere 5 of fore and mid legs with 2 pairs of batonnets, tarsomere 5 of hind leg with 1 pair of batonnets ( Fig. 25 ); fore leg tarsal ratio 2.33– 2.44 (2.38, n = 2); mid leg tarsal ratio 2.65–2.72 (2.68, n = 2); hind leg tarsal ratio 2.75–2.86 (2.80, n = 2); claws slightly shorter than their respective tarsomeres 5, with moderately long basal tooth. Wing ( Fig. 26 ) length 1.75–1.82 (1.78, n = 2) mm, width 0.65–0.68 (0.66, n = 2) mm; costal ratio 0.76–0.77 (0.76, n = 2). Abdomen. ( Fig. 27 ). Broad lateral margins of segment 8 dark brown. Genitalia ( Fig. 28 ): sternite 8 pale, narrow, with U-shaped posteromedian excavation; sternite 9 halves heavily sclerotized, anterior margins straight, posterior margins curved, apices broader, decumbent; sternite 10 pale with 3–4 pairs of setae; cercus rounded, short. Two subequal, ovoid spermathecae with short, moderately wide necks, measuring 90 by 57 (n=2) µ and 87 by 57 (n = 2) µ; a small, spheroid rudimentary third spermatheca measuring 12–15 (13.5, n = 2) µ. Distribution . Brazil ( Rio de Janeiro ). Types . Holotype male, labeled “Holotype Stilobezzia ( Eukraiohelea ) próxima Cazorla and Felippe-Bauer , BRAZIL, Rio de Janeiro, Casimiro de Abreu, Union Biological Reserve (22°25’35” S 42°2’4” W), 07.XI – 13.XII.2013 , "Biota Diptera Fluminense " team col, Malaise trap, (CCER). Allotype female, 1 male paratype with same data as holotype (CCER); other paratypes, 1 male, 1 female with same data except 25.VIII–26.XI.2013 ”, (CCER). Etymology. The species name proxima (from Latin = near), refers to the resemblance of this new species with Stilobezzia ( E. ) elegantula and S ( E .) quasielegantula n. sp. described below. Discussion. Stilobezzia ( E .) proxima n. sp. greatly resembles S . ( E .) elegantula and S. ( E .) quasielegantula n. sp. by its overall pale yellowish coloration, but it readily differs from two dark brown species, S . amnigena and S . dorsofasciata . Females of S . proxima are distinguished from the above two pale species in the subgenus Eukraiohelea by several characters: the larger wing (female wing length 1.33–1.48 mm in elegantula and 1.30– 1.37 mm in S . quasielegantula ); larger spermathecae (spermathecae 50–58 mm long in S . elegantula and 48–65 mm long in quasielegantula ); the absence of dark marks on the abdominal tergites (dark brown L-shaped marks in S . elegantula , dark brown transverse bands with pale spots in S . quasielegantula ), and only one pair of batonnets on tarsomere 5 of the hind leg (two pairs of batonnets on tarsomere 5 of the hind leg in elegantula ). The males of this new species and those of S . quasielegantula have well developed apicolateral processes, which are apparently absent in S . elegantula ; the gonostylus is swollen at midportion, whereas it is subapically swollen in S . quasielegantula , and in S . elegantula is slender; finally, the apicolateral process are short and rounded (they are moderately elongate and lobe¯shaped in S . quasielegantula , but are absent in S . elegantula ).