Two new Cordyceps - like species, Perennicordyceps zongqii sp. nov. (Polycephalomycetaceae) and Purpureocillium zongqii sp. nov. (Ophiocordycipitaceae), in Hypocreales from karst region of China Author Chen, Wan-Hao 0000-0001-7240-6841 Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China & Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China & Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou, China Author Li, Dan Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China Author Liang, Jian-Dong 0000-0002-3939-3900 Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China & Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province, Guiyang 550025, Guizhou, China Author Ren, Xiu-Xiu Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China Author Zhao, Jie-Hong Center for Mycomedicine Research, Basic Medical School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China Author Han, Yan-Feng 0000-0002-8646-3975 Institute of Fungus Resources, Department of Ecology, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China text MycoKeys 2024 2024-11-07 110 141 158 journal article 305025 10.3897/mycokeys.110.135724 542f0541-3372-42a2-8390-27c20bf68355 Purpureocillium zongqii W. H. Chen, Y. F. Han & J. D. Liang sp. nov. Fig. 3 Etymology. In honor of Prof. Zongqi Liang, for his support and guidance in arthropod pathogenic fungi research. Figure 3. Purpureocillium zongqii A , B colony on PDA ( A obverse, B reverse) C – J Phialides and conidia. Scale bars: 10 mm ( A , B ); 10 μm ( C – J ). Type. China • Guizhou Province , Guiyang , Kaiyang County , Monkey-Ear Tiankeng ( 27°5'12.138"N , 107°0'48.42"E ), soil , 19 July 2023 , Wanhao Chen , GZAC TK 04 (dried holotype ), ex-type TK 041 . Description. Colonies on PDA , attaining a diameter of 23–25 mm after 14 days at 25 ° C, white, consisting of a basal felt, floccose hyphal overgrowth, white; reverse yellowish. Hyphae septate, hyaline, smooth-walled, 1.1–1.9 μm wide. Conidiophores 9.3–12.7 × 2.1–2.4 μm, erect, hyaline, verticillately branched, with 1–4 phialides. Phialides 6.8–11.7 × 2.6–4.0 μm, hyaline, cylindrical at base, gradually tapering near the apex. Conidia 2.7–4.2 × 2.0–2.4 μm, hyaline, smooth-walled, thin-walled, ellipsoidal, unicellular, acuminate, arranged in chains not observed. Substrate. Soil. Distribution. Kaiyang County, Guizhou Province , China . Additional strain examined. China • Guizhou Province , Guiyang , Kaiyang County , Monkey-Ear Tiankeng ( 27°5'12.138"N , 107°0'48.42"E ), soil , 19 July 2023 , Wanhao Chen , TK 042 (living culture) . Notes. Purpureocillium zongqii was easily identified as Purpureocillium , based on the BLASTn result in NCBI . Phylogenetic analyses show that P. zongqii has a close relationship to P. lilacinum (Fig. 1 ). However, P. zongqii was easily distinguished from P. lilacinum (conidiophores: 4–6 × 3–4 μm; conidia: ellipsoidal to fusiform, 2–3 × 2–4 μm; phialide, 6–9 × 2.5–3 μm; purple colony) ( Luangsa-ard et al. 2011 ) by its larger conidiophores, larger ellipsoidal conidia, larger phialide and white colony. Thus, the morphological characteristics and molecular phylogenetic results support P. zongqii as a new species.