Review of nematode interactions with hemp (Cannabis sativa) Author Bernard, Ernest C Entomology and Plant Pathology, The University of Tennessee, 370 Plant Biotechnology, Building, 2505 E J Chapman Drive, Knoxville, TN 37996 - 4560. ebernard@utk.edu Author Chaffin, Angel G Pope’s Plant Farm, Maryville, TN. Author Gwinn, Kimberly D Entomology and Plant Pathology, The University of Tennessee, 370 Plant Biotechnology, Building, 2505 E J Chapman Drive, Knoxville, TN 37996 - 4560. text Journal of Nematology 2022 2021-08-23 54 1 1 18 http://dx.doi.org/10.21307/jofnem-2022-002 journal article 298698 10.21307/jofnem-2022-002 c2d976d2-3b31-4091-802d-c11347478fda 2640-396X 11644649 Nematodes and Cannabis sativa secondary metabolites Inflorescences, leaves, and roots of Cannabis sativa contain a wealth of secondary metabolites that are bioactive and have potential as sources of biopesticides ( Andre et al., 2016 ). Cultivars of medicinal C. sativa , CBD-dominant and THC-dominant chemotypes, respectively, are cultivated primarily for the medicinal cannabinoids in the inflorescences (approximately 20% by weight); inflorescences also contain terpenoids and flavonoids but at lower concentrations. Leaves contain less than 10% of the cannabinoids and terpenoids in inflorescences but have higher concentrations of flavonoids ( Jin et al., 2020 ). In general, extracts from inflorescences had greater bioactivity against entomopathogenic nematodes than those from leaves or stems. Extracts of leaves from one THC-dominant chemotype and one CBD-dominant chemotype were attractive to Steinernema feltiae and S. carpocapsae , respectively, whereas extracts from another THCdominant chemotype and another CBD-dominant chemotype were weak repellents to S. carpocapsae ( Laznik et al., 2020 ) . Sterols and triterpenes are the primary components of stem barks and roots ( Jin et al., 2020 ), but other compounds such as aliphatics ( Kornpointner et al., 2021 ), amides ( Yamamoto et al., 1991 ), and cannabinoids ( Gul et al., 2018 ) have also been isolated from roots ( Fig. 3 ). Numerous root compounds impact nematode behavior and development and may influence many of the host-nematode interactions described above.