Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy Author Andersen, Trine Bundgaard Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark Author Rasmussen, Silas Anselm Author Christensen, Søren Brøgger Author Simonsen, Henrik Toft text Phytochemistry 2019 2019-01-31 157 168 174 http://dx.doi.org/10.1016/j.phytochem.2018.10.027 journal article 10.1016/j.phytochem.2018.10.027 1873-3700 10481193 2.2. Characterization of TlTPS820 expressed in N. benthamiana Leaves from N. benthamiana expressing Tl TPS820 were extracted with hexane and analyzed by GC-MS. The analysis yielded a sesquiterpenoid profile identical to Tg TPS2 ( Fig. 1 ). The main product of Tg TPS2 is epikunzeaol ( 1 ) ( Fig. 2 ), thus Tl TPS820 is also an epikunzeaol synthase and an orthologue of Tg TPS2. As described previously, the high injection port temperature at 250 ̊C lead to cope-rearrangement of 1 ( Andersen et al., 2017 ). Thus, 1 was partly degraded to two unknown alcohols .