Diversity of Manota Williston (Diptera, Mycetophilidae) in Ulu Temburong National Park, Brunei Author Sevcik, Jan Author Hippa, Heikki Author Wahab, Rodzay Abdul text ZooKeys 2014 428 57 77 http://dx.doi.org/10.3897/zookeys.428.7912 journal article http://dx.doi.org/10.3897/zookeys.428.7912 1313-2970-428-57 974398800B4745C9BFBCA60A08FE085B 974398800B4745C9BFBCA60A08FE085B Taxon classification Animalia Diptera Mycetophilidae Manota megachaeta sp. n. Fig. 6 Description. Male. Colour. Head brown, face and clypeus paler brown. Antenna brown, scapus and pedicellus paler brown. Mouthparts yellowish. Thorax pale brown, preepisternum 2 ventrally paler yellowish. Legs yellowish. Wing yellowish brown, halter pale brown with blackish knob. Abdomen pale brown, sternites paler than tergites. All setosity pale, yellowish or brownish. Head. Antennal flagellomere 4, Fig. 6A. Palpomere 3 of maxillary palpus with apicomesial thumb-like extension, with 5 apically expanded and curved sensilla; palpomere 4 with parasegment; palpomere 5 is 1.3 times as long as palpomere 4. Number of strong postocular setae ca 9. Thorax. Anepisternum setose, with 33 setae; anterior basalare non-setose; preepisternum 2 setose, with 14 setae, laterotergite non-setose; episternum 3 setose, with ca. 11 setae. Legs. Mid and hind tibial organs absent. Wing. R1 meeting C well on the basal half of the costal margin; the sclerotized part of M2 extending near to the level of the tip of R1; wing length 1.4 (1.5) mm. Hypopygium.Fig. 6 B-E : Sternite 9 about one half of ventral length of gonocoxa, with sharply delimited convex sides, posterior margin convex, anterior margin deeply incised, the setae posteriorly stronger than anteriorly. Ventral mesial margin of gonocoxa simple, concave. Parastylar lobe oblique, subtriangular, with 3 setae anteriorly (at apex). Paraapodemal lobe well exposed in ventral view. The dorsal mesial margin of gonocoxa simple, convex, postero-mesially with a weak setose lobe; posterior margin simple, at posterior margin on a more ventral level with a finger-like lobe bearing two apical and one sub-basal acute curved megasetae. Generally the dorsal setae of gonocoxa similar to the ventral ones. Two juxtagonostylar setae, both long curved acute megasetae arising from a common low inconspicuous basal body. Gonostylus oval, with moderately long setosity ventrally, the dorsal side non-setose except for a transverse row of megasetae on apical half, the megasetae acute and increasing in length towards the mesial margin. Aedeagus short subtriangular, with distinct lateral shoulders, the apex curved ventrad; in Fig. 6C the medially visible part is vas deferens. Hypoproct posteriorly extending to level of base of gonostylus, the ventral part divided into two elongate lobes (sternite 10) with 4 setae each, postero-dorsally with a few both fine and strong setae. Cerci mesially separate. Figure 6. Manota megachaeta sp. n. (holotype). A Antennal flagellomere 4, lateral view B Gonostylus, dorsal view C Aedeagus and hypoproct, ventral view D Hypopygium, ventral view E Hypopygium, dorsal view. Scale 0.1 mm. Female unknown. Discussion. By the following characters Manota megachaeta runs to couplet 65 in the key to Oriental and Palaearctic Manota ( Hippa 2011 ): anepisternum setose, preepisternum 2 setose, laterotergite non-setose, anterior basalare non-setose, juxtagonostylar setae/megasetae simple and not greatly expanded, the dorsal posterior margin of gonocoxa with a cylindrical, not flat, setigerous lobe. This couplet leads to Manota anceps Hippa & Sevcik , 2010, Manota duplex Hippa, 2006, Manota perpusilla Hippa, 2006, Manota vesicaria Hippa, 2009, Manota pellii Hippa, 2008 and Manota hexacatha Hippa & Sevcik , 2010. However, Manota megachaeta is not especially similar to any of them and is easily distinguished by the subapical transverse row of unusually strong megasetae dorsally on the gonostylus. Etymology . The name is a Latinized Greek noun, megachaeta, large seta, referring to the unusually long megasetae on the gonostylus. Types. Holotype. Male, Brunei, Ulu Temburong N. P., Kuala Belalong Field Studies Centre, 4°32'50"N , 115°09'28"E , 7-18.i.2014, primary lowland rainforest, Malaise trap 1 (DOP), J. Sevcik leg. (in UBDC).