A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles roysnellingi Arias-Penna, sp. nov. Figs 195 , 196 Female. Body length 2.22 mm , antenna length 2.46 mm , fore wing length 2.27 mm . Type material. Holotype : COSTA RICA • 1♀ ; 08-SRNP-58202, DHJPAR0034197; Area de Conservacion Guanacaste , Guanacaste , Sector Nuevo Mundo , Camino Pozo Dos ; dry-rain intergrade forest; 728 m ; 10.77111 , -85.3607 ; 09.xi.2008 ; Daniel M. Acuna leg.; caterpillar collected in fifth instar; cocoons adhered to the larval cuticle and formed on 11.xi.2008 ; adult parasitoids emerged on 12.xi.2008 ; ( CNC ) . Paratypes . • 18 ( 3♀ , 4♂ ) (0 ♀ , 11♂ ); 08-SRNP-58202, DHJPAR0034197; same data as for holotype; ( CNC ) . Diagnosis. Ventral margin of fore telotarsus excavated with conspicuous curved seta over this excavation, almost same width throughout, mesoscutum distinctly punctate throughout ( Figs 195F , 196E ), fore wing with vein 2-1A present only proximally as tubular vein, vein 1 cu-a curved, r vein curved, outer side of junction of r and 2RS veins not forming a stub ( Figs 195L , 196K ), medioposterior band of scutellum only very partially overlapping the medioanterior pit of metanotum ( Figs 195G , 196F ), petiole on T1 finely sculptured nly laterally, distally with lateral margins convex ( Figs 195H, I , 196G, H ), surface of metasternum flat or nearly so, precoxal groove deep with lineate sculpture ( Figs 195A, J , 196A, I ), dorsal outer depression on hind coxa present ( Figs 195A, K , 196A, J ), inner margin of eyes diverging slightly at antennal sockets ( Figs 195B , 196B ), propodeum without median longitudinal carina ( Figs 195G , 196F ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Figs 195H, I , 196G, H ). Figure 195. Glyptapanteles roysnellingi sp. nov. female 08-SRNP-58202 DHJPAR0034197 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Genitalia: hypopygium, ovipositor, ovipositor sheaths, lateral view F Mesonotum, dorsal view G Scutellum, metanotum, propodeum, dorsal view H T1-3, dorsal view I, K Metasoma I Dorsal view K Lateral view J Mesosoma, lateral view L Fore and hind wings. Figure 196. Glyptapanteles roysnellingi sp. nov. male 08-SRNP-58202 DHJPAR0034197 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T1-3, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view K, L Wings K Fore L Hind. Coloration ( Fig. 195 A-L ). General body coloration black except scape and pedicel yellow-brown and outer edges both with a lateral brown band; first five-six proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown; labrum, mandibles, and tegulae yellow-brown; glossa, maxillary and labial palps ivory/pale yellow. Eyes and ocelli silver. Fore and middle legs yellow except light brown coxae and claws brown; hind legs yellow except black coxae only distally yellow-brown (coloration is more extensive on the inner side), femora yellow-brown/brown only distally yellow, 2/3 distal of tibiae and tarsomeres brown, although proximally basitarsus with a small yellow band. Petiole on T1 brown, but proximal half lighter than distal half, contours darkened and sublateral areas yellow-brown; T2 with median and adjacent areas brown together forming a rectangle-shaped area, and lateral ends yellow-brown; T3 medially brown forming a dark area coinciding with the width of median and adjacent areas of T2, and lateral ends yellow-brown; T4 and beyond brown; distally each tergum with a narrow hyaline band. In lateral view, T1-3 yellow; T4 and beyond brown. S1-3 yellow; S4 yellow, but medially brown; penultimate sternum and hypopygium brown. Description. Head ( Fig. 195 A-D ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.15:0.07, 0.16:0.07, 0.16:0.070), distal antennal flagellomere longer than penultimate (0.11:0.04, 0.08:0.04), antenna longer than body (2.46, 2.22); antennal scrobes-frons shallow. Face convex with dense fine punctations, interspaces smooth and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.95, 0.11). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma ( Fig. 195A, F, G, J ). Mesosoma dorsoventrally convex. Mesoscutum distal half with a central dent, punctation distinct throughout, interspaces smooth. Scutellum triangular, apex sloped and fused with BS , scutellar punctation distinct peripherally, absent centrally, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM ; ATS demilune with short stubs delineating the area; dorsal ATS groove smooth. Transscutal articulation with large and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM ; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum without median longitudinal carina, proximal half curved with medium-sized sculpture and distal half with a shallow dent at each side of nucha; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by faintly concave/wavy carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with fine rugae and dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove deep with transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus excavated with conspicuous curved seta over this excavation, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.11, 0.06). Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.21, 0.12), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.12, 0.10). Wings ( Fig. 195L ). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with a small smooth area; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A present only proximally as tubular vein; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma ( Fig. 195A, E, H, I, K ). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.28, maximum width 0.15, minimum width 0.09), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.12, length T2 0.12), edges of median area polished and lateral grooves deep, median area broader than long (length 0.12, maximum width 0.17, minimum width 0.06); T2 with scattered pubescence only distally. T3 longer than T2 (0.19, 0.12) and with scattered pubescence only distally. Pubescence on hypopygium scattered. Cocoons. Characteristics unknown. Cocoons adhered to the larval cuticle. Comments. The body is elongate and slim ( Fig. 195A ). Male ( Fig. 196 A-L ). Similar in coloration and shape to female. Etymology. Roy R. Snelling ( 30 September 1934 - 21 April 2008 ) was an internationally renowned American entomologist who studied Hymenoptera , mainly ants, wasps, and bees. He dedicated his professional life to making insect biodiversity better known and appreciated. Distribution. The parasitized caterpillar was collected in Costa Rica , ACG, Sector Nuevo Mundo (Camino Pozo Dos), during November 2008 at 728 m in dry-rain intergrade forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Undetermined species of Geometridae feeding on Bunchosia polystachia ( Malpighiaceae ). Caterpillar was collected in fifth instar.