Lecanicilliumcauligalbarum sp. nov. (Cordycipitaceae, Hypocreales), a novel fungus isolated from a stemborer in the Yao Ren National Forest Mountain Park, Guizhou Author Zhou, Ye-Ming Author Zhi, Jun-Rui Author Ye, Mao Author Zhang, Zhi-Yuan Author Yue, Wen-Bo Author Zou, Xiao text MycoKeys 2018 43 59 74 http://dx.doi.org/10.3897/mycokeys.43.30203 journal article http://dx.doi.org/10.3897/mycokeys.43.30203 1314-4049--59 Lecanicillium cauligalbarum X. Zou, J.R. Zhi & Y.M. Zhou sp. nov. Figure 3 Diagnosis. Characterised by phialides gradually tapering towards the apex, solitary or 2-3 whorls, 9-14.4 x 1.4-1.8 µm . Conidia cylindric, aseptate, 3.6-6.3 x 0.9-1.8 μm . Type. CHINA, Guizhou Province, Sandu county (107°53', 107°58'E; 24°54', 25°59'N, approximately 560-1365 m above sea level), September 2015, Yeming Zhou & Xiao Zou. Sequences from isolated strains (GZUIFRZHJ01 and GZUIFRZHJ02) have been deposited in GenBank (accession numbers to be provided). Description. Colony on PDA 15 mm in diameter after 7 days, 33 mm in diameter after 14 days at 25 °C, colony circular, white, cottony, umbonate, with radiating surface texture from above, with clear radial crack and primrose-yellow from reverse. Mycelium 0.9-1.8 μm wide, hyaline, smooth, septated, branched. Conidiophores usually arising from aerial hyphae, sporulate abundant. Phialides gradually tapering towards the apex, solitary or 2-3 whorls, 9-14.4 x 1.4-1.8 µm . Conidia cylindric, aseptate, 3.6-6.3 x 0.9-1.8 μm . In culture, both phialides and conidia are of similar general shape and size to those found on the host stemborer. Figure 3. Lecanicillium cauligalbarum . a Synnemata emerged from the corpse of a stemborer ( Lepidoptera ) b Culture plate, showing the front (upper) and the back (lower) of the colony, cultured on PDA medium c-e Phialides solitary or in 2-3 whorls f-g Conidia. Scale bars: 10 mm (b, c, e), 5 μm (d, f, g). Host . Stemborer ( Lepidoptera ) hidden amongst wooden sticks. Habitat and distribution. Hidden amongst pieces of wood in humid forests of southwest China. Etymology. The epithet ' cauligalbarum ' refers to the host (stemborer). Teleomorph. Not known. Remarks. With regard to phylogenetic relationships, L. cauligalbarum is closely related to the L. fungicola clade and L. fusisporum . The two strains (GZUIFRZHJ01 and GZUIFRZHJ02) formed a distinct lineage. All Lecanicillium species were included in the phylogenetic analysis except for L. evansii for which sequence data could not be located in public databases, although Zare and Gams (2001) published ITS sequences. The morphological features of L. evansii include brownish-cream to brown reverse, phialides solitary or up to 3-4 per node and two types of the conidia, slightly falcate with a pointed end macroconidia 4.5-7.5 x 0.8-1.2 µm and slightly curved microconidia 2.0-3.0 x 0.8-1.2 µm ( Zare and Gams 2001 ). L. evansii is distinct from L. cauligalbarum , which has conidia of 3.6-6.3 x 0.9-1.8 μm and 9-14.4 x 1.4-1.8 µm phialides. In morphology L. cauligalbarum is is similar to L. aphanocladii , L. attenuatum and L. nodulosum with regard to the short conidiogenous cell (Table 3). However, L. cauligalbarum is distinguished by the pattern of spore production and the frequency of the wheel structure. Table 3. Morphological comparison among Lecanicillium cauligalbarum and the other related species.

Species	Colonies	Conidiogenous cell	Conidia	Refrence
Lecanicillium acerosum	Zare and Gams 2001
L. antillanum	Zare and Gams 2001
L. aphanocladii	Zare and Gams 2001
L. aranearum	Zare and Gams 2001
L. araneicola	Sukarno et al. 2009
L. araneogenum	Chen et al. 2017
L. attenuatum	Zare and Gams 2001
L. cauligalbarum	This work
L. dimorphum	Zare and Gams 2001
L. evansii	Zare and Gams 2001
L. flavidum	Zare and Gams 2008
L. fungicola var. aleophilum	Zare and Gams 2008
L. fungicola var. fungicola	Zare and Gams 2008
L. fusisporum	Zare and Gams 2001
L. kalimantanense	Sukarno et al. 2009
L. lecanii	Zare and Gams 2001
L. longisporum	Zare and Gams 2001
L. muscarium	L. lecanii	Zare and Gams 2001
L. nodulosum	Zare and Gams 2001
L. pissodis	Kope and Leal 2006
L. primulinum	Kaifuchi et al. 2013
L. psalliotae	Zare and Gams 2001
L. restrictum	Crous et al. 2018
L. sabanense	Chirivi-Salomon et al. 2015
L. saksenae	Sukarno et al. 2009
L. subprimulinum	Huang et al. 2018
L. testudineum	Crous et al. 2018
L. tenuipes	Gams et al. 1984 Zare and Gams 2001
L. uredinophilum	Park et al. 2016
L. wallacei	Zare and Gams 2001 2008