Two new Oriental species of Paramanota Tuomikoski (Diptera: Mycetophilidae), with DNA sequence data Author Hippa, Heikki Author Kaspřák, David Author Kahar, Siti Rafhiah Haji Abd Author Ševčík, Jan text Raffles Bulletin of Zoology 2016 2016-12-07 64 360 367 journal article 10.5281/zenodo.5355716 2345-7600 5355716 20D2CA85-5EC3-4145-8599-9AEEA565F07A Paramanota rodzayi , new species ( Figs. 1A–E ) Type material. Holotype . Male (in UBDC ). BRUNEI , Ulu Temburong N.P., Kuala Belalong Field Studies Centre [primary lowland rainforest], Malaise trap ID3, 4°33’N 115°10’E , coll. D. Kaspřák & M. Mantič, 25 January–7 February 2015 . Female. Unknown. Etymology. This species is named after Rodzay Abdul Wahab (Universiti Brunei Darussalam and Institute for Biodiversity and Environmental Research in Brunei ), who provided us field facilities to study Diptera in Brunei Darussalam and helped to arrange all the necessary permissions. Distribution. Brunei . This species represents the first record of the genus Paramanota from the island of Borneo. Concerning the subfamily Manotinae , only several species of Manota and Eumanota humeralis Edwards, 1933 had previously been recorded from Borneo (cf. Edwards, 1933 , Hippa & Ševčík, 2010 , Ševčík et al., 2014 ). Description. Male. Colour . Head yellowish, vertex and occiput darker brown, antennal flagellum becoming darker towards the apex; setae and other vestiture dark. Thorax yellowish, scutum, scutellum and the medial part of mediotergite brown, prothoracic pleura slightly darker than other pleural parts; setae dark. Legs yellowish, hind femur slightly infuscated ventrally near apex, the setae and other vestiture dark which makes the apical part of tibiae and all of tarsi seem dark under low magnification. Wing greyish brown; haltere yellowish with black knob. Abdomen grey brown, tergites and tergites concolorous, setae dark. Head. Similar to fig. 7a in Hippa et al. (2005) but the number of facial setae higher. Antennal flagellomere 4, Fig. 1E . The two basal palpomeres of maxillary palp similar to fig. 7a in Hippa et al. (2005) but the setae longer and stronger, the two apical segments broken off from the specimen. The number of strong postocular setae 6–7, occiput with a group of 4 strong setae latero-ventrally. Thorax similar to Fig. 8a in Hippa et al. (2005) . Legs . Front tibial organ not well visible in the specimen, apparently consisting of a transverse row of 3 setae. Wing similar to fig. 1F in Hippa (2010), stM and the basal part of M 1 and M 2 visible as shades. Wing length 1.7 (1.9) mm. Hypopygium , Figs. 1A–D : Gonocoxae ventrally separated by a membranous area, each side with a very large lobe which is posteriorly extending nearly as far as the gonostylus, with the posterior part simple, not divided into sub-lobes; the ventral surface of the lobe as well as the other ventral parts of gonocoxa evenly covered with strong setae, the dorsal surface of the lobe with a postero-mesial area of numerous megasetae subequal in size. Gonocoxa dorsally is simple with setae similar to those of the ventral side. Tergite 9 simple, with a few strong setae. Tergite 10 distinct, discernible as a separate sclerite, with a few lateral setae. Cercus simple. The hypoproct with three setae (one on the left side, two on the right side). Gonostylus with a dorsal lobe, a ventral lobe and a median lobe, each with difficultly observable largely membranous sub-lobes; the dorsal lobe in dorsal view subtriangular, with apical and mesial membranous part showing a striated or lamellar structure; the ventral lobe narrow, transverse, at anterior margin with a long comb-like row of narrow lamellae, the median lobe narrow, transverse, with an apical transverse comb-like row of narrow lamellae. Aedeagus subtriangular. Fig. 1. Paramanota rodzayi , new species (holotype). A, Hypopygium, dorsal view; B, Hypopygium, ventral view; C, Ventral and median lobe of gonostylus, anteroventral view; D, Aedeagus with associated structures, dorsal view; E, Antennal flagellomere 4, lateral view. Scale bar = 0.1 mm. cr = cercus, gs d = dorsal lobe of gonostylus, gs m = median lobe of gonostylus, gs v = ventral lobe of gonostylus, gx = gonocoxa, gx l = ventral gonocoxal lobe, hp = hypoproct, tg 9 = tergite 9, tg 10 = tergite 10. Table 1. GenBank accession numbers for the species included in the molecular analyses

Taxa	COI	GenBank accession numbers 12S	28S	BOLD process ID
Eumanota humeralis	KC435643	KC435536	KC435608	GBDP14447-13
Eumanota suthepensis	KC435644	KC435537	K C435609	GBDP14446-13
Manota mabokeensis	KC435647	KC435540	KC435612	GBDP14443-13
Manota mazumbaiensis	KC435648	KC435541	K C435613	GBDP14442-13
Manota unifurcata	KC435649	KC435542	KC435614	GBDP14441-13
Paramanota furcillata	KC435656	KC435550	KC 435622	GBDP14434-13
Paramanota orientalis	KC435657	KC435551	KC 435623	GBDP14433-13
Paramanota peninsulae	KX713156	KX713154	KX 713155	JSPAR003-16
Paramanota rodzayi	KU496908	KU496909	KU496910	JSPAR001-15
Promanota malaisei	KC435661	KC435557	KC435629	JSPAR002-15

Discussion. Possible relationships of the new species. In the key to the species of Paramanota (Hippa, 2010) , P. rodzayi fits couplet 1 including only P. orientalis because the ventral gonocoxal lobe is posteriorly simple, not divided into a more lateral and a more mesial sub-lobes. In this respect P. rodzayi is similar to P. trilobata , the other new species described in the present paper. Both these species are distinguished from P. orientalis by having all the megasetae on the dorsal side of the ventral gonocoxal lobe short, the longest ones at most one fourth the width of the lobe while in P. orientalis they are double of that length. Paramanota rodzayi is distinguished from all other known Paramanota by its very short antennal flagellomeres, being twice broader than long instead of being about as long as broad. In the mount of the holotype , tergite 10 is well visible as a separate sclerite even if it is partly fused with tergite 9. In the other cases we have seen only the lateral part has been discernible (as in Fig. 2A ) and have been described as posterolateral part of tergite 9. DNA sequences. GenBank accession numbers for two mitochondrial (12S, COI) and one nuclear (28S) gene markers are presented in Table 1 .