Arumatia, a new genus of Diapheromerinae stick insects (Insecta, Phasmatodea) from Brazil, with the description of five new species and a reassessment of species misplaced in Australian genera Author Ghirotto, Victor Morais 905002F8-3DF8-4640-87C2-81C10F9CBC2B Museu de Zoologia, Universidade de São Paulo, São Paulo, Brazil. Projeto Phasma, São Paulo, Brazil. Departamento de Ciências Biológicas, Universidade Estadual Paulista “ Júlio de Mesquita Filho ”, Assis, Brazil. Departamento de Zoologia, Universidade de Brasília, Brasília, Brazil. Victorghirotto@hotmail.com Author Crispino, Edgar Blois 672BF116-8086-4783-9FB8-1149A99914D5 Museu de Zoologia, Universidade de São Paulo, São Paulo, Brazil. Projeto Phasma, São Paulo, Brazil. Departamento de Ciências Biológicas, Universidade Estadual Paulista “ Júlio de Mesquita Filho ”, Assis, Brazil. Departamento de Zoologia, Universidade de Brasília, Brasília, Brazil. edgarbcrispino@gmail.com Author Engelking, Phillip Watzke 64491A5C-6DFF-4E82-9F1B-F8BB1BDEB3FF engelking.phillip@gmail.com Author Neves, Pedro Alvaro Barbosa Aguiar 430B96C4-79F8-4997-AAA4-61289FC08E1D alvaro3dssa@gmail.com Author Góis, Júlia de BCF3039B-CE53-4119-AD3A-E9C93BA11A9D juliadegois@gmail.com Author Chiquetto-Machado, Pedro Ivo BD1800B1-06FA-493A-BB8D-9C0C371CC9E5 Museu de Zoologia, Universidade de São Paulo, São Paulo, Brazil. Projeto Phasma, São Paulo, Brazil. Departamento de Ciências Biológicas, Universidade Estadual Paulista “ Júlio de Mesquita Filho ”, Assis, Brazil. Departamento de Zoologia, Universidade de Brasília, Brasília, Brazil. pedroivocm@gmail.com text European Journal of Taxonomy 2022 2022-07-04 827 827 1 85 http://zoobank.org/8b6f1573-b627-4c62-94ca-db0f1146ed2c journal article 89522 10.5852/ejt.2022.827.1849 7cad31f6-9bbf-434c-a411-14cef4448ff0 2118-9773 6795618 8B6F1573-B627-4C62-94CA-DB0F1146ED2C Arumatia aramatia Ghirotto gen. et sp. nov. urn:lsid:zoobank.org:act: 404881CA-488A-40C9-9110-BDF60ABD5788 Figs 25–33 , 43 , 52 , 53F Diagnosis Females From Arumatia dubia gen. et comb. nov. and A. fulgens gen. et comb. nov. it differs by the more elongate head, the shorter median segment in relation to the metanotum, the longer terga VIII–X, the round posterior margin of tergum X, the slightly longer subgenital plate, the presence of a praeopercular organ and additionally from A. dubia the longer gonapophyses VIII. From A. anyami gen. et sp. nov. it differs by the more elongate head, the longer median segment in relation to the metanotum, the presence of stouter and spiniform setae at the apex of the carinae of the tibiae, the round posterior margin of tergum X, the slightly shorter subgenital plate, the presence of a praeopercular organ, the longer gonoplac and gonapophyses IX and shorter gonapophyses VIII. From A. crassicercata gen. et sp. nov. it differs by the more elongate head, the shorter median segment in relation to the metanotum, round posterior margin of tergum X, the longer cerci, and the longer gonapophyses IX. From A. motenata gen. et sp. nov. and A. diamante gen. et sp. nov. it differs by the less elongate head, the longer median segment in relation to the metanotum, the presence of stronger spiniform setae on the carinae of tarsi and on the apex of mid tibiae, the absence of an apical sessile spine on the apex of the ventral, antero- and posteroventral carinae of the tibiae, the longer cerci and subgenital plate and shorter gonapophyses VIII. Males From Arumatia motenata gen. et sp. nov. , the only other species with a known male, it differs by the head with convex sides in dorsal view rather than parallel to concave, the presence of one to few stouter spiniform setae at the end of all carinae, the absence of an apical sessile spine on the apex of ventral, antero- and posteroventral carinae of the tibiae, tergum IX longer than X, poculum elongate and conical, pointed in lateral view, tergum X without lateral expansions, and thorn pads restricted to the ventral area of the posterior margin of tergum X. Eggs Eggs of Arumatia aramatia gen. et sp. nov. can be differentiated from known eggs of all other species by the fewer distinct, slightly sinuous ridges of the capitulum forming a shape resembling a turtle shell as well as the large and not constricted opercular collar which is shared only with the eggs of A. diamante gen. et sp. nov. Etymology This species is named in reference to Aramatiá , a variant of the indigenous name for stick insects in Brazil , Arumatiá , which inspired the name of the new genus of which this is the type-species. To be treated as a noun in apposition. Material examined Holotype BRAZIL • ♀ ; Tocantins , Porto Nacional , Luzimangues district , at margins of Ribeirão Santa Luzia ; 10°03′40.1″ S , 48°25′10.1″ W ; 15 Aug. 2002 ; D. Pavan and J.P. Silva leg.; “vegetação fechada” [dense vegetation] ; campanha VII; gride C; MZUSP 1352 . Paratype BRAZIL • 1 ♂ ; same collection data as for holotype; 6–14 Dec. 2002 ; campanha IX; MZUSP 1322 . Remarks Eggs were obtained from inside the abdomen of the only known female and might differ in some details from laid eggs. For comparison, analysis of eggs of Arumatia dubia gen. et comb. nov. extracted from the same region of the abdomen revealed that in this species the capitulum and micropylar plate were already formed but the whitish dorsal stains were not present and the texture of the capsule was sometimes smooth, still not formed. Description Female ( holotype , MZUSP 1352) MEASUREMENTS (in mm). Body (without cerci) 86.1, head 5.0, antennae at least 12.0, pronotum 3.3, mesonotum 22.6, metanotum 7.0, median segment 5.2, abdomen (excluding median segment) 43.0, cercus 5.8, profemur 21.8, protibia 23.3, mesofemur 15.0, mesotibia 15.8, metafemur 20.0, metatibia at least 22.2. COLOUR ( Fig. 25 ). Known only from aged specimens preserved in alcohol. Entirely brown to yellowish beige, lighter in the legs. Body with scattered dark sprinkled stains and dorsal longitudinal medial dark line running across mesonotum, menatonum, median segment and sternum II. HEAD ( Fig. 26 ). Fairly elongate, smooth with sparse setae mostly on dorsal region, vertex flat but very gently convex at posterior margin to fit underneath the pronotum, frontal convexity developed, frontal suture round ( Fig. 26A–B ). Eyes small, slightly elongate, approximately 0.2 × as long as head. Cervix covering significantly more than half of head, cervical sclerites developed, gula bearing setae and covering less than half of cervix ( Fig. 26C ). Subgena fairly narrow with posterior projection ca height of middle of eye. Submentum narrow and slightly curved towards posterior, mentum simple and prementum somewhat wide. Glossa elongate and rounded, paraglossa larger, roundly falcate and reaching labrum. Lacinia with three distal teeth, one large medially and two smaller laterally, mesal edge bearing around 6 large setae. Galea slightly elongate, roundly elliptical, bearing long setae, apically with dense semicircular band of hairy and large microtrichia. Galealobulus present, small, widely round and basally fused to galea. Maxillary palpal segments cylindrical, labial slightly widened. Clypeus wide, approximately trapezoidal. Labrum strongly notched anteromedially, asymmetric. Antennae filiform, broken. Scapus ca 1.8 × as long as wide, basally and apically slightly constricted in dorsal view, slightly compressed dorsoventrally. Pedicellus subglobose, large and more than half length of scapus ( Fig. 26A ). THORAX ( Figs 25–26 , 27A ). Smooth with scattered setae. Pronotum longer than wide, slightly constricted pre-medially and slightly wider from transverse sulcus towards anterior margin, fairly flat in lateral view, transverse sulcus conspicuous and almost straight, longitudinal sulcus conspicuous and distinct. Paranota curved, slightly widened medially and longer than wide, procoxopleurite apically round and ventrally gently concave ( Fig. 26 ). Probasisternum tapering towards anterior, profurcasternum round ( Fig. 26C ). Mesothorax 4.8× as long as prothorax. Mesonotum with pair of weak lateral carinae, mesepisternum lanceolate and regularly widening posteriorly. Mesepimeron slightly elongate, pointing towards posterior and slightly exceeding end of mesothorax, mesocoxopleurite indistinct, mesofurca Y-shaped. Metathorax continuing pair of lateral carinae of mesonotum, metepisternum long and similar to mesepisternum, metepimeron extremely elongate, extending through the entire length of median segment, posteriorly Fig. 25. Arumatia aramatia Ghirotto gen. et sp. nov. , holotype, ♀ (MZUSP 1352). A . Dorsal view. B . Lateral view. Scale bar: 10 mm . pointing and slightly exceeding end of median segment ( Fig. 27A ). Metacoxopleurite very discrete, elongate, metafurca Y-shaped. Metanotum 1.2× as long as median segment ( Fig. 27A ). LEGS ( Figs 25 , 27B–F ). Slender. Hindlegs extending beyond epiproct, at least slightly shorter than anterior legs, midlegs distinctly shorter. Coxae smooth. Profemur with distinct basal curvature. Femora and tibiae with five carinae with few sparse setae among them ( Fig. 27B–F ). Carinae of profemora and protibiae distinctly keeled, mid and hind femora and tibiae with slightly less prominent keels. Antero- and posteroventral carinae of femora with round apical prominence. Carinae of all femora, tibiae and tarsi bearing row of short, stout setae. Last few setae of all carinae of protibiae and last five setae of ventral carinae, last three of antero- and posteroventral carinae and last two of antero- and postero-dorsal carinae of mesotibiae stouter and spiniform ( Fig. 27B–E ). Metatibia apex broken off ( Fig. 27F ). In tarsi, setae on carinae distributed across about three quarters to four fifths of length and on ventral and posteroventral carinae of probasitarsi longer and only slightly stouter while setae on ventral, antero- and posteroventral carinae of mesobasitarsi stouter (only slightly on anteroventral carinae) ( Fig. 27B–E ). Probasitarsi very elongate and significantly longer than respective following tarsomeres combined, Fig. 26. Arumatia aramatia Ghirotto gen. et sp. nov. , holotype, ♀ (MZUSP 1352), head and prothorax morphology. A . Dorsal view. B . Lateral view. C . Ventral view. Abbreviations: Ant =antennomere; BsIII=pro- and mesothoracic basisterna;Cly = clypeus; Csc= cervical sclerite; Cx =coxa;Cxp=coxopleurite; Eye= compound eye; Fc =frontal convexity; Fs =prothoracic furcasternite; Gu =gula; Lb=labrum; Md =mandible; Msn =mesonotum; Par= paranota; Pd =pedicellus; Plb =labial palpus; Pmx =maxillary palpus; Prn =pronotum; Sc =scapus; Sme =submentum. Scale bars: 1 mm . Fig. 27. Arumatia aramatia Ghirotto gen. et sp. nov. , holotype, ♀ (MZUSP 1352), thorax and leg morphology. A . Metathorax and median segment in dorsal view, arrows indicate the length of each segment. B . Left fore tarsus, ventral view. C . Detail of right fore tibia and tarsus (mirrored), prolateral view. D . Right mid tarsus (mirrored), prolateral view, arrows showing stronger apical spines. E . Detail of left mid tibia and tarsus, ventral view, arrow showing stronger apical spines. F . Detail of broken apex of left tibia, dorsal view. Abbreviations: Ar =arolium; Cda =dorsal anterior carina; Cdp=dorsal posterior carina; Cl =claw; Cva =ventral anterior carina; Cve =ventral carina; Cvp; ventral posterior carina; Epm =epimeron; Eps=episternum; FTa = fore tarsomere; Mds =median segment; Msn =mesonotum; MTa =mid tarsomere; Mtn =metanotum; Tg=tergum; Tib=tibia. Scale bars: 1 mm. mesobasitarsi as long as respective following tarsomeres combined ( Fig. 27B–E ), with hairy setae restricted to apical portion on ventrolateral patches ( Fig. 27B, D ). Remaining tarsomeres with setae on ventrolateral patches in portions not covered by euplantulae. Tarsomeres I–III with a discrete dorsal round apical projection. Arolium round and broad, bearing setae dorsally. Pretarsal claws symmetrical, dorsally and outwardly bearing setae. Euplantulae well developed in all tarsomeres, composed of two symmetrical pads separated by median groove in tarsomeres I–III, of single pointing domed pad in tarsomeres IV, and of single flattened lobed pad in tarsomeres V. Euplantulae present only apically at Fig. 28. Arumatia aramatia Ghirotto gen. et sp. nov. , holotype, ♀ (MZUSP 1352), terminalia morphology. A . Dorsal view. B . Lateral view. C . Ventral view. D–E . Detail of external genitalia, lateral view (D), ventral view (E). F . Detail of praeopercular organ, ventral view.Abbreviations: Ce=cercus; Ep=epiproct; Gap= gonapophysis; Gpl =gonoplac; Pre =praeopercular organ; Prp= paraproct; Sp=subgenital plate; St =sternum; Tg=tergum. Scale bars: 1 mm . tarsomeres I–II, covering ca half length of tarsomeres III, covering two thirds length of tarsomeres IV and covering almost entire ventral surface of tarsomeres V ( Fig. 27B ). ABDOMEN ( Figs 25 , 27A , 28 ). External surface as in thorax, bearing few setae across entire length. Median segment slightly shorter than metanotum ( Fig. 27A ). Combined length of segments II–X as long as combined length of head, thorax and median segment. Terga II–VII and sterna II–VIII bearing discrete lateral carinae near lateral margins. All segments longer than wide ( Fig. 25 ). Segment II ca as long as III and VII, segments III–V slightly increasing in size in relation to anterior segment, segment VI ca as long as V, tergum VII shorter than on preceding segment, and longer than VIII, terga X and IX same size, slightly shorter than VIII. Terga II–V around same width, wider than following terga, VI wider than following terga, VIII–X around same width ( Fig. 28A ). Tergum X somewhat conical in lateral view ( Fig. 28B ), in dorsal view almost parallel-sided but gently tapering towards posterior, posterior margin straight with round lateral margins ( Fig. 28A ). Epiproct domed in dorsal view, dorsoventrally flattened, distinctly prominent visible dorsally and laterally ( Fig. 28A–B ). Paraprocts elongate, posteriorly acute, straight and bearing setae on posterior margin, laterally bearing cerci, not concealing them from ventral or lateral view ( Fig. 28B–C ). Cerci extremely elongate, straight, gently laterally compressed, basally narrower fitting paraprocts, pointing to posterior, gradually tapering, slightly longer than terga IX, X and epiproct combined ( Fig. 28A–C ). Cerci bear four types of setae, shorter setae very discrete, round and not protruding. Praeopercular organ small, round, brownish and posteriorly with short longitudinal sulcus continuing across posterior margin of sternum VII ( Fig. 28B–C, F ). Subgenital plate lanceolate, fairly acute, reaching ⅔ of length of tergum X, bearing two parasagittal carinae beginning on anterior margin and running half length of segment before becoming flatter and more setose, gradually tapering towards posterior and totally covering gonapophyses and gonoplac ( Fig. 28B–C ). Gonapophyses and gonoplac flattened, dorsoventrally for gonapophyses VIII, lateroventrally for IX and laterally for Fig. 29. Arumatia aramatia Ghirotto gen. et sp. nov. , paratype, ♂ (MZUSP 1322). A . Dorsal view. B . Ventral view. Scale bar: 10 mm . gonoplac ( Fig. 28D–E ). Gonapophyses VIII longer than IX, VIII almost reaching tergum X, IX reaching ca ¾ of length of tergum IX, VIII linear to lorate, IX conical, tapering towards posterior ( Fig. 28D–E ). Gonapophyses IX ventrally folded to fit within gonapophyses VIII. Gonangulum distinctly reduced, flat, not lobed. Gonoplac significantly elongate, widened basally, subsequently lorate, bearing setae, exceeding both gonapophyses and exceeding anterior margin of tergum X ( Fig. 28D–E ). Male paratype (MZUSP 1322) MEASUREMENTS (in mm). Body 56.7, head 2.7, antennae at least 15.0, pronotum 2.2, mesonotum 13.8, metanotum 5.4, median segment 4.1, abdomen (excluding median segment) 28.5, cercus 1.0, profemur -, protibia -, mesofemur 11.8, mesotibia 13.1, metafemur 16.7, metatibia 19.8. COLOUR ( Fig. 29 ). Known only from aged specimen preserved in alcohol. Entirely dark brown to yellowish beige, lighter in legs, darker than female. Head, pronotum and mesonotum with two lateral dark lines ( Fig. 30B ). Fig. 30. Arumatia aramatia Ghirotto gen. et sp. nov. , paratype, ♂ (MZUSP 1322), head and prothorax morphology. A . Head and prothorax in dorsal view. B . Head and prothorax in lateral view. C . Head and prothorax in ventral view. Abbreviations: Ant = antennomere; BsI-II =pro- and mesothoracic basisterna; Cly =clypeus; Csc =cervical sclerite; Cx =coxa; Cxp =coxopleurite; Eye=compound eye; Fc =frontal convexity; Fs=prothoracic furcasternite; Gu =gula; Lb =labrum; Md=mandible; Msn =mesonotum; Par =paranota; Pd =pedicellus; Plb =labial palpus; Pmx=maxillary palpus; Prn =pronotum; Sc =scapus; Sg = subgena; Sme =submentum. Scale bars: 1 mm . HEAD ( Fig. 30 ). Similar to female except: eyes, scapus and pedicel larger, head slightly widening from posterior margin to behind eyes in dorsal view ( Fig. 30A ). Palpal segments larger, labial less widened. THORAX ( Figs 29 , 31A ). Similar to female except: significantly thinner, more elongate than that of female. Mesothorax 6.6× as long as prothorax. Mesocoxopleurite larger and triangular. LEGS ( Figs 29 , 31B–D ). Forelegs not present in paratype , the only known male of species. Similar to female except: legs longer and thinner than those of female ( Fig. 29 ). All setae generally longer than those of female ( Fig. 31B–D ). Setae on carinae of meso- and metafemora slightly sparser, longer, on carinae of meso- and metatibiae slightly longer, stouter, and apical spiniform setae of carinae of tibiae only slightly stouter than remaining setae ( Fig. 31D ). Meso- and metabasitarsi longer than respective following tarsomeres combined ( Fig. 31B–C ). Metabasitarsi with stouter setae on ventral, antero- and Fig. 31. Arumatia aramatia Ghirotto gen. et sp. nov. , paratype, ♂ (MZUSP 1322), thorax and leg morphology. A . Metathorax and median segment in dorsal view, arrows indicate the length of each segment. B . Right mid tarsus, ventral view. C . Right hind tarsus (mirrored), prolateral view. D . Detail of right hind tibia and tarsus (mirrored), latero-ventral view, arrow showing stronger apical spines. Abbreviations: Ar= arolium; Cda =dorsal anterior carina; Cl=claw; Cva =ventral anterior carina; Cve =ventral carina; Cvp; ventral posterior carina; Epm =epimeron; Eps= episternum; HTa =hind tarsomere; Mds =median segment; Msn =mesonotum; MTa =mid tarsomere; Mtn=metanotum; Tg= tergum; Tib, tibia. Scale bars: A–C= 1 mm ; D = 0.5 mm . posteroventral carinae, stronger on ventral carinae. Ventrolateral hairy setae of tarsomeres denser. Euplantulae shorter. ABDOMEN ( Figs 29 , 31A , 32 ). Thinner and more elongate than that of female. Median segment anteriorly with two parasagittal ovoid stains ( Fig. 31A ), lateral carinae absent. Terga II and VI about same length, both slightly shorter than III–V, tergum VI shorter than VII, tergum VII longer than VIII, terga VIII and IX about same length, X shorter. Terga II–V about same width, tergum VI slightly shorter, tergum VII anteriorly as wide as VI and slightly widened posteriorly, tergum VIII slightly wider than VII and IX ( Fig 32A ). Tergum X wider than IX, about as long as wide, slightly widened towards posterior, sides convex in dorsal view and with posterior margin emarginate forming two round edges ( Fig. 32A ). Thorn pads at ventral area of posterior margin of tergum X bearing ca 32 small to large inwardly curved short, sclerotized teeth ( Fig. 32C ). Tergum VIII and sternum VIII separated, not fused ( Fig. 32B, D ). Sternum VIII partly fused with sternum IX, border between both sterna shown as asymmetric sulcus curving towards posterior on right side ( Fig. 32E ). Sternum IX reduced with anterior portion indistinct; only shown as poculum. Poculum reaching around ¾ length of tergum IX, due to asymmetry of sulcus poculum Fig. 32. Arumatia aramatia Ghirotto gen. et sp. nov. , paratype, ♂ (MZUSP 1322), terminalia morphology. A . Dorsal view. B . Left side lateral view. C . Detail of thorn pads, ventro-posterior view. D . Right side lateral view. E . Ventral view. F . Detail of vomer, ventral view. Abbreviations: Ce=cercus; Ep=epiproct; Po= poculum; Prp=paraproct; St = sternum; Tg=tergum; Tp=thorn pads; Vo =vomer. Scale bars: A–B, D–E = 1 mm ; C, F = 0.5 mm . shorter at right side than left side ( Fig. 32B, D ). Poculum slightly elongate, conical in lateral view, round in ventral view, posterior margin medially with wide emarginate short curvature towards anterior ( Fig. 32B, D–E ). Cerci slightly shorter than tergum X, inwardly curved, with round apex ( Fig. 32B, D–E ). Epiproct small, hidden from dorsal and lateral view ( Fig 32F ). Vomer elongate, convex, terminal hook elongate and strongly sclerotized, acute, conical, single pointed and curving upwards ( Fig. 32F ). MALE GENITALIA ( Fig. 43 , left side). Male genitalia or phallic organ composed of dorsalmost sclerite (dorsal sclerite), wide lobe with fine granulation originating ventrally from dorsal sclerite (longitudinal lobe), and smaller and shorter lobe basally, slightly ventrally, on right side, the (right) basal lobe. Dorsal sclerite very elongate, somewhat hook-shaped, curved to left in dorsal view and penetrating body cavity. Anterior portion penetrating body narrower. Posterior portion wider, connecting to rest of genitalia, and apically further bending ventrally while continuing left curve of whole structure, basally near anterior portion very short, round projection pointing anteriorly. Longitudinal lobe bends accompanying dorsal sclerite, and forming apicalmost round lobe, small elongate and thin lobe at right side and central foldings. Longitudinal lobe basally bearing round weak internal sclerotization near dorsal sclerite ( Fig. 43E–H ). Basal lobe conical, flattened and densely granulated. Egg ( Fig. 33 ) Egg not fully formed, retrieved from inside female holotype . Measurements in mm (N = 3): length 2.5–2.6, height 1.9, width 1.5. Relatively small, ovoid in lateral view, not constricted at opercular Fig. 33. Arumatia aramatia Ghirotto gen. et sp. nov. , egg morphology from the holotype female. A–B . Lateral view, arrowhead pointing at bristles on opercular collar (A). C–D . Dorsal view. E . Lateral view, slightly tilted towards dorsal. F–G . Detail of operculum, anterior view. Abbreviations: Cap =capitulum; Mcup =micropylar cup; Mip=micropylar plate; MLi =median line; Opc =operculum. Scale bars: 0.5 mm . collar, gently compressed laterally and ca 1.6 × as long as wide and 1.4× as long as tall ( Fig. 33A–D ). Capsule surface smooth but with finely punctuated appearance ( Fig. 33B, D ). Colour yellowish orange. Micropylar plate very elongate, occupying large area of dorsal region, with round margins and almost parallel-sided ( Fig. 33C–D ). Micropylar cup small, rounded and only slightly elevated. Median line short and of same colour and elevation as elevated margin of micropylar plate, almost reaching polar area ( Fig. 33C–D ). Opercular collar very wide, as wide as surrounding capsule, smooth with minute and delicate long bristles along edge ( Fig. 33A ). Operculum elliptical, with sinuous and somewhat radial elevations with amber texture, in shape resembling a turtle shell ( Fig. 33A, F–G ). Distribution ( Fig. 52 ) Known only from the type locality, Luzimangues District, along Santa Luzia River, in Porto Nacional, Tocantins , Brazil . This area is in the Cerrado domain and the types were found in savannah formations ( Fig. 53F ).