Phylogeny and taxonomy of two new species in Dictyosporiaceae (Pleosporales, Dothideomycetes) from Guizhou, China Author Feng, Yao 0000-0002-0888-8775 School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang, Guizhou 550025, China & Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550025, China Author Liu, Zuo-Yi 0000-0001-5348-8458 Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550025, China Author Chen, Xiao-Fang https://orcid.org/0009-0000-9962-7644 Bijie Medical College, Bijie, Guizhou 551700, China Author Yang, Mi-Lian 0000-0003-3511-3630 School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang, Guizhou 550025, China Author Zhang, Zhi-Yuan 0000-0003-2031-7518 School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang, Guizhou 550025, China Author Chen, Ya-Ya 0000-0002-8293-168X College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, Guizhou 550025, China & Institute of Crop Germplasm Resources, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550025, China text MycoKeys 2024 2024-06-27 106 251 264 journal article 299036 10.3897/mycokeys.106.125693 d6bdb1d6-d5c5-44b2-aadf-fd7979397734 Pseudocoleophoma paraphysoidea Y. Feng & Z. Y. Liu sp. nov. Fig. 3 Etymology. The epithet refers to the species having paraphyses. Holotype . GZAAS 21-0197 . Diagnosis. Saprobic on decaying wood in terrestrial habitats, and immersed in host epidermis. At maturity, the fruiting body breaks through host epidermis. Sexual morph : undetermined. Asexual morph: Conidiomata dark brown to black, pycnidial, solitary to gregarious, globose to subglobose, apapillate, ostiolate. Conidiomatal wall comprising several layers of cells of textura angularis , with inner layers comprising hyaline to dark brown and outer layers composed of dark brown to black cells. Conidiogenous cells 11–27 × 3–5 μm (av. 21 × 4 μm, n = 20), hyaline, enteroblastic, phialidic, with minute collarette, doliiform, ampulliform, arising from the innermost layer of the pycnidial wall, intermixed with hyaline, filamentous, septate paraphyses. Conidia 12–15 (- 23) × 2–4 μm (av. 14 × 3 μm, n = 30), hyaline, smooth, cylindrical to subcylindrical or fusiform, straight or slightly curved, aseptate, guttulate at both ends. Culture characteristics. Conidia germinating on WA within 12 h and germ tubes produced from the basal end. After transfer to the PDA , the colonies grew rapidly, reaching 5 cm diam. in 1 week at 25 ° C. Part of the mycelia grew on the surface of the medium, compact, violet, with a light-colored rim, and part of the mycelia remained immersed in the medium. The central area of the colony on the back was reddish-brown, the middle white, and the edge light-colored. Morphology of Pseudocoleophoma paraphysoidea ( GZAAS 21-0197 , holotype) A, B appearance of pycnidia on host C peridium D germinating conidium E paraphyses F, G culture H, I conidiogenous cells and conidia J – M conidia. Scale bars: 20 μm ( C ); 10 μm ( D, H, I ); 5 μm ( J – M ). Material examined. China , Guizhou Province , Guiyang City , Guizhou Academy of Agricultural Sciences , on dead culms of an unidentified plant , 18 June 2018 , Zuo-Peng Liu , NK- 1 ( holotype GZAAS 21-0197 , ex-type living culture GZCC 21-0711 ) . China , Guizhou Province , Xingyi City , on dead culms of an unidentified plant , 7 August 2019 , Yao Feng XY 19-13 ( paratype GZAAS 21-0198 , living culture GZCC 21-0712 ) . Notes. The multi-locus phylogenetic analyses showed that the new isolates GZCC 21-0711 and GZCC 21-0711 ( Pseudocoleophoma paraphysoidea ) formed a single clade and clustered together with high support value 81 / 0.94 (Fig. 1 ). Pseudocoleophoma paraphysoidea differs from P. bauhiniae in its conidiogenous cells intermixed with paraphyses, longer conidiogenous cells (11–27 × 3–5 μm vs. 2.5–5.5 × 2–3 µm) and larger conidia (12–15 × 2–4 μm vs. 7.5–11 × 2–3 µm) ( Jayasiri et al. 2019 ). In addition, they can be distinguished by their low sequence similarities. In a comparison of LSU , ITS , SSU , and tef 1 - α nucleotides, P. paraphysoidea (type strain GZCC 21-0711 ) has 99 %, 97 %, 99 % and 93 % similarity, in LSU (789 / 795 bp, no gap), ITS (490 / 503 bp, 2 gaps), SSU (1008 / 1010 bp, one gap), and tef 1 - α (815 / 878 bp, no gap), which is different from P. bauhiniae (Type strain MLFUCC 17-2586 ).