A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene sebastianus Brölemann, 1902 Figs 31F , 121–122 , 172B , 178L , 187 ; Supp. file 4: Fig. 201C Pseudonannolene longicornis var. sebastianus Brölemann, 1902a: 126 , pl. vi figs 128–133. Pseudonannolene longicornis var. sebastianus – Brölemann 1909: 57 . Pseudonannolene sebastiana – Verhoeff 1943: 269 , figs 23–27 (misidentified specimens from Viçosa, Minas Gerais , Brazil ). — Jeekel 2004: 91 . Pseudonannolene sebastianus – Mauriès 1987: 173 , figs 6–8 ( lectotype and paralectotype designations). — Gallo & Bichuette 2020: 36 . Diagnosis Males of P. sebastianus resemble those of P. halophila , P. maritima , P. patagonica , and P. insularis by having large and subrectangular coxae on the first leg-pair ( Fig. 122A ) and a suboval penis ( Fig. 122C ), but differing by having the solenomere rounded, seminal apophysis located medially ( Fig. 122D ), and internal branch without horizontal plate ( Fig. 122D–F ). Etymology Although unspecified, the name is evidently an adjective referring to the locality where the type material was found, Ilhabela (formerly Ilha de São Sebastião). Material examined Paralectotypes (total: 2 ♂♂, 2 ♀♀, 1 immature) BRAZIL • 2 ♂♂, 2 ♀♀, 1 ♀ immature ; São Paulo , Ilhabela (formerly Ilha de São Sebastião ); [ -23.812818 , -45.362573 ]; 1 m a.s.l. ; Sep. 1896 ; MZSP . Other material (total: 18 ♂♂ , 26 ♀♀ , 62 immatures ) BRAZIL – São Paulo • 9 ♂♂ , 5 ♀♀ , 1 ♀ immature; Ilhabela ; [ -23.818664 , -45.368161 ]; 1 m a.s.l. ; Aug. 1950 ; H. Urban leg.; IBSP 7902 • 1 ♂ ; same collection data as for preceding; IBSP 7905 • 1 ♂ ; same locality data as for preceding; 9–15 Oct. 2001 ; Equipe Biota leg.; IBSP 1389 • 1 ♀ ; same collection data as for preceding; IBSP 7890 • 1 ♀ ; same collection data as for preceding; IBSP 1393 • 2 ♀♀ , 1 immature ; same collection data as for preceding; IBSP 1386 • 1 ♂ ; same collection data as for preceding; IBSP 1390 • 1 ♂ ; same collection data as for preceding; IBSP 1396 • 1 ♀ immature; same collection data as for preceding; IBSP 7789 • 1 ♀ , 1 ♀ immature; same collection data as for preceding; IBSP 1391 • 1 ♀ ; same collection data as for preceding; IBSP 1392 • 1 ♀ ; same collection data as for preceding; IBSP 7891 • 1 ♂ ; Ubatuba , Fazenda Angelim ; [ -23.433713 , -45.083857 ]; 5 m a.s.l. ; Dec. 2003 ; IBSP 3651 • 1 ♂ , 6 ♀♀ , 4 immatures ; Ilha de Dentro ; 19 Jun. 1994 ; C.F. Vieira and A. Eterovic leg.; IBSP 1110 • 2 ♀♀ , 4 ♂♂ immatures, 10 ♀♀ immatures; Ilha da Pesca ; 2–10 Sep. 1994 ; C.F. Vieira and A. Eterovic leg.; IBSP 1117 • 2 ♂♂ immatures, 1 ♀ immature; Ilha Anchieta ; [ -23.550426 , -45.066637 ]; 162 m a.s.l. ; 23–30 Jul. 2001 ; Equipe Biota leg.; IBSP 1424 • 4 ♂♂ immatures, 3 ♀♀ immatures; same collection data as for preceding; IBSP 1435 • 2 ♂♂ immatures, 5 ♀♀ immatures; same collection data as for preceding; IBSP 7904 • 1 ♀ , 4 ♀♀ immatures; same collection data as for preceding; IBSP 1404 • 1 ♂ immature; same collection data as for preceding; IBSP 1416 • 1 ♀ , 1♀ immature; same collection data as for preceding; IBSP 1420 • 1 ♂ immature; same collection data as for preceding; IBSP 1425 • 1 ♀ immature; same collection data as for preceding; IBSP 1421 • 1 ♂ immature; same collection data as for preceding; IBSP 1411 • 1 ♂ immature; same collection data as for preceding; IBSP 1406 • 1 ♂ immature; same collection data as for preceding; IBSP 1419 • 1 ♀ immature; same collection data as for preceding; IBSP 1428 • 1 ♂ immature, 1 ♀ immature; same collection data as for preceding; IBSP 1415 • 1 ♂ immature; same collection data as for preceding; IBSP 7903 • 1 ♀ , 2 ♀♀ immatures; same collection data as for preceding; IBSP 1432 • 1 ♂ immature; same collection data as for preceding; IBSP 1427 • 1 ♂ immature, 1 ♀ immature; same collection data as for preceding; IBSP 1403 • 3 ♂♂ immatures; same collection data as for preceding; IBSP 1405 • 2 ♂♂ , 2 ♀♀ ; Santos ; [ -23.967882 , -46.328886 ]; 6 m a.s.l. ; Sep. 1896 ; MZSP • 1 ♀ ; Cubatão ; [ -23.894019 , -46.424589 ]; 6 m a.s.l. ; 1990; MZSP • 1 ♂ ; São Paulo , Belém ; [ -23.547131 , -46.591176 ]; 750 m a.s.l. ; 1990; MZSP . Descriptive notes MEASUREMENTS . 55–58 body rings (1 apodous + telson). Males: body length 49.5–63.5 mm ; maximum midbody diameter 3–3.3 mm . Females: body length 59–90 mm ; maximum midbody diameter 3.4– 4.8 mm . COLOR . Body color brownish grey; head and collum darker; antennae greyish; prozonites anteriorly greyish; metazonites with a medial darker band and a posterior brownish; legs brownish. HEAD . Antennae short ( Fig. 121A ), just reaching back to end of ring 5 when extended dorsally; relative antennomere lengths 1<2≈3>4>5≈6>7. Mandibular cardo with ventral margin narrow. Ommatidial cluster well-developed, elliptical; ca 35 ommatidia in 5 rows. BODY RINGS . Collum with lateral lobes broadly rounded, with ca 9 deep striae ( Fig. 121A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae above ozopore in anterior body rings. Anterior sterna in midbody rings subrectangular, with 8–9 transverse striae ( Fig. 172B ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) elongated (as long as the sum of remaining podomere lengths), subrectangular, with the base arched, densely setose ( Fig. 122A ); prefemoral process ( prf ) elongated and as wide as half of prefemur, subcylindrical, densely setose along the entire ventral region ( Fig. 122B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) as long as the sum of remaining podomere lengths, rounded; penis ( pn ) located at proximal region, rounded, extended basally ( Fig. 122C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) elongated, almost twice as long as telopodite, with the base arched; antero-posteriorly flattened ( Fig. 122D–F ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder ( sh ) rounded. Telopodite ( tp ) almost as wide as gcx ( Fig. 122D ), with short laterad projection; solenomere ( sl ) rounded, with apicomesal process ( amp ) subtriangular; ectal process absent; sa located at medial portion, visible apically. Internal branch ( ib ) subtriangular, narrow, surrounding basally tp as a shield; ib with setae along its entire margin nearly exceeding apically seminal region of sl ( Fig. 122D–F ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 178L ); internal valve subtriangular, with mesal region rounded; operculum narrow, slightly curved ectad; external valve wide, subtriangular. Distribution The species is widely distributed in the Atlantic Forest of the coastal region of São Paulo State , Brazil , including some continental islands ( Fig. 187 ). Comments The lectotype and paralectotypes ( two males ) from Ilha de São Sebastião deposited at the Muséum national d’histoire naturelle, Paris, France (MNHN), were not examined during this study.