Belgopeltula belgica (Vincx & Gourbault, 1992) gen. et comb. nov. and Mudwigglus micramphidium sp. nov. from the west coast of Sweden, and reappraisal of the genus Pseudaraeolaimus Chitwood, 1951 (Nematoda: Araeolaimida: Diplopeltidae) Author Holovachov, Oleksandr text European Journal of Taxonomy 2017 2017-12-19 383 1 21 journal article 21908 10.5852/ejt.2017.383 97b45cd3-b0b6-44ca-8c76-a7c81bf4b555 2118-9773 3839807 8D18E64D-6E01-43CC-9A97-BD5C4D2160E1 Key to genera of Diplopeltidae See Table 4 for additional diagnostic characters. 1. Amphidial fovea a transverse slit; cuticle with ten longitudinal ridges .... Edalonema Andrássy, 2007 – Amphidial fovea double-loop-shaped in male, unispiral in female .................. Belgopeltula gen. nov. – Amphidial fovea unispiral ................................................................................................................ 2 – Amphidial fovea loop-shaped ........................................................................................................... 4 2. Basal bulb absent; freshwater or terrestrial ........................................ Cylindrolaimus de Man, 1880 – Basal bulb present; marine ................................................................................................................ 3 3. Single anterior ovary ................................................................ Intasia Tchesunov & Miljutina, 2008 – Two ovaries (anterior and posterior) ....................................................... Southerniella Allgén, 1932 4. Oral opening shifted to the ventral body side ............................ Morlaxia Vincx & Gourbault, 1988 – Oral opening apical or shifted to the dorsal body side ..................................................................... 5 5. Basal pharyngeal bulb present; ovaries antidromously reflexed ............... Mudwigglus Leduc, 2013 – Basal pharyngeal bulb absent; ovaries outstretched ......................................................................... 6 6. Lateral alae present, in some species fused with the ventral limb of the amphid ............................... ................................................................................................................... Campylaimus Cobb, 1920 – Lateral alae absent ............................................................................................................................ 7 7. Amphid in a shape of rounded loop .................................................................................................. 8 – Amphid in a shape of elongated loop ............................................................................................. 10 8. Anterior body region abruptly narrowing; amphid at a distance equal to more than five labial region diameters from the anterior end ............................................. Metaraeolaimoides De Coninck, 1936 – Anterior body region cylindrical or subcylindrical; amphid at a distance equal to less than two labial region diameters from the anterior end ............................................................................................. 9 9. Buccal cavity cup-shaped; pronounced sexual dimorphism in the amphid size (large in males and small in females) .................................................................................. Pararaeolaimus Timm, 1961 – Buccal cavity conical; no sexual dimorphism in the amphid size .......... Araeolaimus de Man, 1888 Table 4. Selected diagnostic characters of genera of the family Diplopeltidae Filipjev, 1918 based on literature data supplemented with recent observations (modified after Tchesunov & Miljutina 2008 ).

Araeolaimus	Belgopeltula gen. nov.	Cylindrolaimus	Campylaimus	Diplopeltis	Diplopeltula	Edalonema
Cuticular ornamentation	absent	absent	absent	longitudinal striae present or absent	absent	longitudinal striae present or absent	ten longitudinal ridges
Oral opening	apical	subdorsal	apical	subdorsal	apical	apical or subdorsal	apical
Amphideal fovea shape	rounded loop	double loop in male, unispiral in female	unispiral	elongated loop	elongated loop	elongated loop	transverse slit
Subcuticular plate supporting amphid	absent	absent	absent	absent	present	present or absent	absent
Ocelli	present	absent	absent	absent	present	absent	absent
Cervical setae	present	absent	absent	absent	present	absent	absent
Buccal cavity	conical	minute	tubular	minute	minute	cylindrical	tubular
Pharynx	with swelling at anterior ⅓	with fusiform corpus and narrow postcorpus	cylindrical	cylindrical	cylindrical	cylindrical	cylindrical
Female gonad	didelphic	didelphic	didelphic or monodelphic-prodelphic	didelphic	didelphic	didelphic	monodelphic- prodelphic
Ovaries	outstretched	reflexed	outstretched	outstretched	outstretched	outstretched	outstretched
Caudal glands open via	spinneret	spinneret	spinneret	spinneret	spinneret	three pores	caudal glands absent
Intasia	Metaraeolaimoides	Morlaxia	Mudwigglus	Pararaeolaimus	Pseudaraeolaimus	Southerniella
Cuticular ornamentation	absent	absent	absent	absent	absent	absent	absent
Oral opening	apical	apical	subventral	apical	apical or subdorsal	apical	apical
Amphideal fovea shape	unispiral	rounded loop	rounded loop	elongated loop	rounded loop	elongated loop	unispiral
Subcuticular plate supporting amphid	absent	absent	absent	absent	absent	absent	absent
Ocelli	absent	present	absent	absent	absent	present or absent	absent
Cervical setae	absent	present	absent	absent	absent	present	absent
Buccal cavity	tubular	minute	minute	barrel-shape	cup-shaped	minute	tubular
Pharynx	with basal bulb	subcylindrical	with basal bulb	with basal bulb	cylindrical	glandular posteriorly	with basal bulb
Female gonad	monodelphic- prodelphic	didelphic	monodelphic-prodelphic	didelphic	didelphic	didelphic	didelphic
Ovaries	outstretched	outstretched	outstretched	reflexed	outstretched	outstretched	outstretched
Caudal glands open via	spinneret	?	spinneret	three pores or spinneret	three pores	spinneret	spinneret

10. Cervical (paramphideal) setae absent; caudal glands open via three separate pores .......................... ................................................................................................................. Diplopeltula Gerlach, 1950 – Cervical (paramphideal) setae present; caudal glands open via common spinneret ........................11 11. Amphid located on a well-developed refractive subcuticular plate .................................................... .......................................................................................... Diplopeltis Cobb in Stiles & Hassal, 1905 – Refractive subcuticular plate absent .......................................... Pseudaraeolaimus Chitwood, 1951