Taxonomy on crane flies in family Tipulidae and Limoniidae (Diptera: Tipuloidea) from Yaoluoping National Nature Reserve, Anhui, China Author Men, Qiulei Author Lu, Zhiyong Author Sheng, Pingping Author Gui, Xiang Author Wang, Zhizhong Author Mu, Dan text Zoological Systematics 2017 42 4 476 489 journal article 10.11865/zs.201720 2095-6827 5366761 965EE80F-11BE-430D-91CA-325C66A9FD1D Nephrotoma impigra anqingensis Men , subsp. nov. ( Figs 16–30 ) Diagnosis. The new subspecies has brown occipital mark. Prescutum is yellow with three black stripes. Wings are transparent, yellowish-gray with stigma light brown. Abdominal tergites are yellow, have brown stripes, tergites six and seven of which are almost wholly covered with brown spot. Tergite nine is produced into a slender, straight, nail-like projection on lateral angle, the posterior margin of which is densely covered with black points. Description. Male body length 9.1–9.5 mm , wing 9.7–8.0 mm, antenna 4.0– 4.2 mm . Head. Rostrum yellow with nasus black. Nasus densely covered with black long hairs. Antennae with scape elongated, yellowish-brown, expanded apically; pedicel very short, pot-shaped, light brown; flagellum black, each flagellomere enlarged at base, with black verticils distinctly shorter than the flagellomeres from which they arise. Palpi brownish-black, the outer two segments somewhat paler. Head yellow, occipital mark brown, widened basally and acute apically, the point extending to the base of vertical tubercle ( Fig. 16 ). Thorax. Pronotum yellow medially, black laterally, and then changing to brown basally ( Figs 17–18 ). Prescutum yellow with three black stripes, the median one broadened apically and gradually narrowed to base ( Fig. 18 ). Scutum yellow, lobes variegated by black stripes ( Fig. 17 ). Scutellum yellow, almost wholly covered with a black spot ( Fig. 17 ). Postnotum yellow with a brown median area ( Fig. 17 ). Pleura yellow, with yellowish-brown areas on anepisternum, caudal margins of anepisternum, katepisternum and ventral meron ( Fig. 18 ). Halteres with stem yellowish-brown, knob dark brown. Legs with coxae and trochanters yellowish-brown; femora dark brown except the restricted paler base; tibiae and tarsi dark brown. Wing transparent, yellowish-gray; stigma oval, light brown; Rs very short, subequal to basal part of R 4+5 , cell r 1 without stigmal trichia, cell m 1 short-petiolate ( Fig. 19 ). Abdominal tergites yellow, with brown stripes medially, tergites six and seven almost wholly covered with brown spot, lateral stripe lacking ( Fig. 20 ). Sternites one to seven yellow, sternite eight brown. Hypopygium yellowish-brown. Ninth tergite produced into a slender, straight, nail-like projection on lateral angle, each with a series of four black points ( Figs 23–25 ). Posterior margin of ninth tergite with an incision medially, densely covered with small black points ( Figs 24–25 ). Outer gonostylus spindle-shaped ( Figs 23–24, 28 ). Inner gonostylus with two black beaks ( Figs 23–24, 26–28 ). Sternite eight with posterior margin transverse, unarmed ( Fig. 26 ). Semen pump with compressor apodeme V-shaped, each arm expended apically ( Fig. 30 ). Posterior immovable apodeme narrow, shorter than compressor apodeme ( Fig. 29 ). Anterior immovable apodeme with arms acute apically, shorter than compressor apodeme ( Fig. 29 ). Aedeagus relatively thick, tubular, at least 3.5 times longer than semen pump ( Fig. 29 ). Female body length 10.2–10.5 mm , wing 10.5–10.8 mm , antenna 4.0– 4.2 mm . Figures 8–15. Holorusia henana . 8. Hypopygium, lateral view. 9. Hypopygium, dorsal view. 10. Hypopygium, ventral view. 11. Inner gonostylus, inner lateral view. 12. Inner gonostylus, outer lateral view. 13. Inner gonostylus, dorsal view. 14. Semen pump, lateral view. 15. Compressor apodeme, dorsal view. Scale bars: 8–10= 2.0 mm; 11–14 = 0.5 mm; 15= 0.25 mm. Figures 16–22. Nephrotoma impigra anqingensis Men , subsp. nov. 16. Head, dorsal view. 17. Thorax, dorsal view. 18. Thorax, lateral view. 19. Wing. 20. Abdomen (male), lateral view. 21. Ovipositor, lateral view. 22. Ovipositor, dorsal view. Scale bars: 16 = 0.5 mm; 17–22= 1.0 mm. Colors of head, thorax and abdomen similar to those of male. Ovipositor yellow. Sternite ten slightly shorter than cerci, parallel in lateral margins in dorsal view ( Figs 21–22 ). Cerci long, acinacifoliate, surpassing the end of hypovalva ( Figs 21–22 ). Hypovalva simple, extending to nearly three quarters length of cerci ( Figs 21–22 ). Material examined. Holotype . ♂ . China , Anhui , Yaoluoping National Nature Reserve , Yaoluoping Village ( 30°59'N , 116°04'E ), 26 August 2016 , coll. Qiulei Men , Zhizhong Wang. Paratypes . 18♀ 25♂ , Anhui , Yaoluoping National Nature Reserve , Yaoluoping Village ( 30°59'N , 116°04'E ), 26 August 2016 , coll. Qiulei Men. 2♀ 3♂ , Anhui , Yaoluoping National Nature Reserve , Duozhijian ( 30°58'N , 116°06'E ), 24 August 2016 , coll. Qiulei Men. Distribution. China ( Anhui ). Etymology. The specific epithet is a noun ‘ anqing ’ with Latin suffix ‘ ensis ’, referring to the type locality of the new subspecies. Remarks. The new subspecies differs from the nominal form by venation with cell m 1 short-petiolate (long in nominal subspecies as described by Alexander, 1935b: 138 ), differs from another subspecies, Nephrotoma impigra fulvovittata ( Savchenko, 1964 ) by smaller size, black stripes on prescutum and dark brown femora (stripes on prescutum reddish brown, femora reddish in N. i. fulvovittata as described in Savchenko, 1964: 892 ).