A revision of the minor species group in the millipede genus Nannaria Chamberlin, 1918 (Diplopoda, Polydesmida, Xystodesmidae) Author Means, Jackson C. https://orcid.org/0000-0001-7377-0696 Virginia Tech, Department of Entomology, Blacksburg, Virginia 24061, USA mjacks4@vt.edu Author Hennen, Derek A. https://orcid.org/0000-0001-7005-1151 Virginia Tech, Department of Entomology, Blacksburg, Virginia 24061, USA Author Marek, Paul E. https://orcid.org/0000-0002-7048-2514 Virginia Tech, Department of Entomology, Blacksburg, Virginia 24061, USA text ZooKeys 2021 2021-04-13 1030 1 180 http://dx.doi.org/10.3897/zookeys.1030.62544 journal article http://dx.doi.org/10.3897/zookeys.1030.62544 1313-2970-1030-1 875199397EEE5F7898EA1DB25DA62D25 Nannaria cingulata sp. nov. Figs 45 , 46 , 47 Vernacular name: "The Swamp-Dwelling Twisted-Claw Millipede" Material examined. Holotype : United States - Virginia • ♂ ; Shenandoah County , Massanutten Trail nr. Signal Knob parking off Route 678; 38.9332°N , - 78.3207°W ; elev. 250 m ; 24 Feb. 2017 ; hand collected; C. Harden leg.; VTEC MPE02324 . Paratypes : United States - Virginia • 11 ♂♂ ; same collection data as holotype; VTEC MPE02325 -2335 • 11 ♂♂ ; same collection data as holotype; VMNH MPE02336 -45, 3668 • 1 ♀ ; Clarke County , nr. Mount Weather ; 39.0717°N , - 77.9120°W ; elev. 326 m ; 22 June 2017 ; hand collected; J. Means , D. Hennen leg.; VTEC MPE01881 . Other material. United States - Virginia • 1 ♂ ; Chesterfield County , Pocahontas State Park ; 37.3679°N , - 77.5755°W ; 11 May 2002 ; A. Evans leg.; VMNH NAN0221 • 1 ♂ ; Clarke County , 1.7 miles by road N of Ashby Gap , U.S. Rt. 50 crossing of Blue Ridge; 39.0133°N , - 77.9622°W ; 10 May 1958 ; W. Highton leg.; VMNH NAN0227 • 2 ♂ ; Fairfax County , Hemlock Overlook Regional Park challenge course; 38.7666°N , - 77.4104°W ; elev. 101 m ; 8 Apr. 2017 ; hand collected; P. Marek , C. Hall leg.; VTEC MPE02438 , 2447 • 2 ♂ ; Fauquier County , Sky Meadows State park , near junction of North Ridge Trail and Appalachian Trail ; 38.9850°N , - 77.9900°W ; elev. 549 m ; 21 Apr. 2017 ; hand collected; C. Harden leg.; VTEC MPE02473 , 77 • 2 ♂♂ ; Frederick County , Albin , ca. 3 mi. W of Winchester on VA. Hy 679; 39.2216°N , - 78.1988°W ; 10 Apr. 1990 ; LFCC class leg.; VMNH NAN0225 • 1 ♂ ; Prince Edward County , Hampden-Sydney College ; 37.2381°N , - 78.4606°W ; 20 April 1983 ; J. White leg.; VMNH NAN0041 • 3 ♂♂ ; NAN0230 Prince George County , Lanham ; 38.9669°N , - 76.8622°W ; 1 June ; H. Loomis leg.; VMNH NAN0230 • 1 ♂ ; Shenandoah County , Massanuten Trail ca. 2.5 mi from Signal Knob parking lot by Rte. 698; 38.9324°N , - 78.3249°W ; elev. 260 m ; 24 Feb. 2017 ; hand collected; C. Harden , G. Chapman leg.; VTEC MPE02348 • 2 ♂♂ ; Shenandoah County , George Washington National Forest near Eliz. Furn. Group Cmpgrd ; 38.9310°N , - 78.3220°W ; elev. 233 m ; 26 Feb. 2017 ; hand collected; C. Harden leg.; VTEC MPE03437 , 03438 • 3 ♂♂ ; Shenandoah County , George Washington National Forest , Botts Trail , south of Elizabeth Furnace ; 38.9240°N , - 78.3280°W ; elev. 250 m ; 21 Apr. 2017 ; hand collected; C. Harden leg.; VTEC MPE02475 , 76, 3673 • 2 ♂♂ ; Shenandoah County , dry creak near swamp just past Elizabeth Furnace Campground ; 38.9122°N , - 78.3326°W ; elev. 248 m ; 22 Apr. 2018 ; hand collected; J. Means , N. Larson leg.; VTEC MPE03899 , 03900 • 1 ♀ ; same collection data as preceding; VTEC, MPE03901 • 1 ♂ ; Suffolk County , Magnolia, US Hys. 58-460; 36.7458°N , - 76.5408°W ; 23 Feb. 1954 ; R. Ragot leg.; VMNH NAN0229 • 1 ♂ ; Warren County , Smithsonian Conservation Biology Institute , High Knob Mountain ; 38.8930°N , - 78.1370°W ; elev. 396 m ; 13 Mar. 2017 ; hand collected; C. Harden leg.; VTEC MPE02463 • 6 ♂♂ ; Warren County , Racetrack Hill , gravel road/field; 38.8915°N , - 78.1680°W ; elev. 305 m ; 4 Apr. 2017 ; hand collected; C. Harden leg.; VTEC MPE02453 -56, 2786, 2787 • 1 ♂ ; Warren County , SCBI, High Knob Mountain ; 38.8930°N , - 78.1370°W ; elev. 390 m ; 6 Mar. 2017 ; hand collected; C. Harden leg.; VTEC MPE03744 • 1 ♂ ; Warren County , upper reaches of Stokes Branch 0.17 mi N of dirt forest road terminus, off Rte 613 nr jct w/608; 38.9156°N , - 78.2994°W ; elev. 265 m ; 12 Feb. 2018 ; hand collected; C. Harden leg.; VTEC MPE03885 • 26 ♂♂ ; Warren County , 2 mi SE Front Royal , DF site at NZP-CRC, oak woods on hillside; 38.8975°N , - 78.1683°W ; 1 May 1996 ; VMNH Survey leg.; VMNH NAN0232 • 4 ♂ ; York County , Cheatham Annex NSD Jones Mill Pond ; 37.2850°N , - 76.6416°W ; 17 Nov. 1989 ; Buhlmann , Pague leg.; VMNH NAN0222 • 1 ♂ ; same collection data as preceding; 2 Feb. 1997 ; S. Roble leg.; VMNH NAN0223 • 1 ♂ ; same collection data as preceding; 2 Nov. 1989 ; K. Buhlmann leg.; VMNH, NAN0224 • 2 ♂♂ ; same collection data as preceding; 20 Feb. 1990 ; VDNH survey leg.; VMNH NAN0228 • 1 ♂ ; same collection data as preceding; 17 Nov. 1989 ; K. Buhlmann leg.; VMNH NAN0359; SCAU - West Virginia • 2 ♂♂ , 2 ♀♀ ; Berkeley County , Sleepy Creek Hunt Area ; 39.5057°N , - 78.1695°W ; 6 May 1968 ; P. Martinat leg.; NCSM NAN0449, 450. For detailed collection data see Suppl. material 7 . Diagnosis. Adult males of Nannaria cingulata sp. nov. are distinct from other Nannaria and the sympatric N. shenandoa , based on the following combination of characters: Gonopods . Gonopodal acropodite gently curving medially before bending dorsomedially, not ventromedially as in N. paupertas sp. nov. and N. shenandoa . Distal zone short, bent dorsomedially at 45°, not bent dorsomedially at 90° as in N. paupertas sp. nov., or curving posterolaterally as in N. shenandoa . Acropodite tip blunt with small, lobed lateral flange (Fig. 45C , red arrow), not with large, pointed flange as in N. shenandoa . Telopodite basal zone height <1/2 length of acropodite with lateral bulge (Fig. 45A , red triangle), not greatly reduced as in N. shenandoa , or lacking lateral bulge as in N. paupertas sp. nov. Acropodite shaft swollen before apex, with cingulum (Fig. 45A , red rectangle), not simple as in N. shenandoa , or with medial flange as in N. paupertas sp. nov. Prefemur with prefemoral process straight for first 3/4, and bent ventrally at 90° in last 1/4, not bent ventromedially as in N. paupertas sp. nov., or curving laterally as in N. shenandoa . Prefemoral process ca. 1/2 length of acropodite, not 2/3 length as in N. paupertas sp. nov. or N. shenandoa . Prefemoral spine greatly reduced to small, tooth-like projection (Fig. 45C , red oval), not prominent, acicular as in N. paupertas sp. nov., or absent as in N. shenandoa . Color . Tergites with orange paranotal spots (Fig. 46A ) and occasionally faint orange stripes (Fig. 46B ). Dark to light brown background. Dorsum of collum smooth with orange margin. Figure 45. Nannaria cingulata sp. nov. holotype ♂ (VTEC, MPE02324) left gonopod A anterior view; red triangle indicates basal lateral bulge; red rectangle indicates cingulum B medial view C posterior view; red arrow indicates small lateral flange on acropodite tip; red oval indicates reduced prefemoral spine. Scale bar: 0.5 mm. Figure 46. Nannaria cingulata sp. nov. coloration A holotype ♂ (VTEC, MPE02324) orange paranota B non-type ♀ (VTEC, MPE01881) faint orange stripes. Scale bars: 4.0 mm. Measurements. ♂ holotype (VTEC, MPE02324): BL = 24.7, CW = 3.9, IW = 2.1, ISW = 0.8, B11W = 4.4, B11H = 2.9. ♀ paratype (VTEC, MPE01881): BL = 28.6, CW = 3.9, IW = 2.7, ISW = 0.9, B11W = 4.8, B11H = 3.3. Variation. The only Nannaria cingulata sp. nov. individual known from Prince Edward Co., Virginia has a cephalically directed prefemoral process and a pronounced prefemoral spine (Fig. 47 , red arrow). Figure 47. Nannaria cingulata sp. nov. (VMNH, NAN0041) from Prince Edward Co., Virginia. Red arrow indicates projected prefemoral spine. Scale bar: 0.5 mm. Distribution. Nannaria cingulata sp. nov. has a more extensive range than most species in the Nannaria minor species group and can be found from eastern West Virginia south to central and southeastern Virginia, and Maryland in the Washington D.C. metropolitan area (West Virginia: Berkley County; Virginia: Frederick, Clarke, Shenandoah, Warren, Fairfax, Chesterfield, Prince Edward, Gloucester counties, and the City of Suffolk; Suppl. material 7; Fig. 128 ). Distribution area: 28,825 km2; status: WRE. Ecology. The majority of Nannaria cingulata sp. nov. specimens collected by C. Harden have been taken from mesic hardwood forests at night and were found walking on top of leaf litter and on man-made paths, or were collected using pitfall traps. Several museum specimens of N. cingulata sp. nov. lack ecological notes. Etymology. This species is named for the presence of a cingulum, a linear groove and possible point of flexion, on its gonopod, a characteristic unique within Nannaria . The specific name is a feminized adjective derived from the Latin cingulatus for belted. Type locality. United States, Virginia, Shenandoah County, Massanutten Trail nr. Signal Knob parking off Route 678, 38.9332°N , - 78.3207°W .