Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrus grandis cv. " Tomentosa " in China Author Liu, Jia-Wei Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China Author Manawasinghe, Ishara S. https://orcid.org/0000-0001-5730-3596 Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China ishara9017@gmail.com Author Liao, Xuan-Ni Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China Author Mao, Jin Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China Author Dong, Zhang-Yong Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China Author Jayawardena, Ruvishika S. https://orcid.org/0000-0001-7702-4885 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand Author Wanasinghe, Dhanushka N. https://orcid.org/0000-0003-1759-3933 School of Science, Mae Fah Luang University, Chiang Rai, Thailand Author Shu, Yong-Xin Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand Author Luo, Mei Innovative Institute for Plant Health / Key laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agricul-ture and Rural Affairs, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China 08luomei@163.com text MycoKeys 2023 2023-02-23 95 163 188 http://dx.doi.org/10.3897/mycokeys.95.87121 journal article http://dx.doi.org/10.3897/mycokeys.95.87121 1314-4049-95-163 9D55E0913AFE50E38B5E9A119F266467 Colletotrichum tomentosae J.W. Liu, Manawas. & M. Luo sp. nov. Fig. 2 Etymology. The epithet refers to the cultivar of the host plant - Citrus grandis cv. "Tomentosa" . Holotype. ZHKUCC 21-0103. Description. Endophytic in C. grandis cv. "Tomentosa" leaf. Sexual morph : not observed. Asexual morph : Conidiophores 20-40 x 3-5 μm (x- = 29.8 +/- 5.5 x 3.7 +/- 0.6 μm , n = 30), hyaline, cylindrical, 1-3-celled, unbranched or branched at the base. Conidia 10-20 x 3-6 μm (x- = 12.5 +/- 1.6 x 4.4 +/- 0.6 μm , n = 50), 1-2-guttulate, aseptate, straight, hyaline, smooth-walled, middle part cylindrical both ends obtuse, middle part occasionally shrinkage or bulging. Appressoria 5-15 x 5-10 μm (x- = 10 +/- 1.8 x 7 +/- 1.5 μm , n = 50) solitary or in loose groups, light brown to medium brown, Ellipsoidal to subcircular or irregular-shaped. Cultural characteristics. Colonies on PDA reach 70 mm diam. in seven days, with 10-11 mm/day (x- = 10 mm, n = 6) growth rate. Colonies flat with entire margin, floccose cottony, surface grey in the centre with glaucous margin. Reverse buff in the centre with off-white margin. Material examined. China , Guangdong Province , Huazhou , isolated from a healthy leaf of Citrus grandis cv. "Tomentosa" , May 2019 , Y.X. Shu , (dried cultures ZHKU 21-0088 holotype ); ex-type culture ZHKUCC 21-0103 (= CGMCC 3.24128), ex-isotype ZHKUCC 21-0104, ZHKUCC 22-0041 ). Notes. In the phylogenetic analysis of combined six genes, Colletotrichum tomentosae formed an independent clade (Fig. 1 ). This species is phylogenetically distinct from C. syzygicola . Morphologically, appressoria developed by C. syzygicola (DNCL021; Udayanga et al. (2013) ) are longer than C. tomentosae (5-15 x 18-24 μm vs. 18-24 μm ). Colletotrichum tomentosae has longer conidiophores (20-40 x 3-5 vs. 12-16 x 4-5 μm ). This species can be distinguished from C. syzygicola by 32 nucleotide differences (1/511 in the ITS region, 2/229 in the gapdh region, 7/242 in the act region and 22/906 in the gs region). The PHI test revealed no significant evidence for a recombination (p = 1.0) event amongst C. syzygicola and its closely-related taxa (Fig. 3 ). Therefore, we have described this fungus as a novel species. Figure 3. PHI analysis of combined ITS, gapdh , chs-1 , act and β- tubulin sequence data. PHI test result (Φw) <0.05 indicates significant recombination within the dataset.