Taxonomic review of Cerozodus Bigot, 1857 (Diptera: Asilidae: Asilinae) with the description of two new species Author Camargo, Alexssandro 0000-0001-7408-7223 Instituto Nacional de Pesquisas da Amazônia-INPA, Coordenação de Biodiversidade-COBIO, Manaus, Amazonas, Brazil. alexssandroecs @ gmail. com; https: // orcid. org / 0000 - 0001 - 7408 - 7223 alexssandroecs@gmail.com Author Vieira, Rodrigo Secretaria de Estado de Educação do Governo do Amazonas-SEDUC, Manaus, Amazonas, Brazil / Instituto Nacional de Pesquisas da Amazônia-INPA, Coordenação de Biodiversidade- COBIO, Manaus, Amazonas, Brazil. Author Rafael, José Albertino 0000-0002-0170-0514 Instituto Nacional de Pesquisas da Amazônia-INPA, Coordenação de Biodiversidade-COBIO, Manaus, Amazonas, Brazil. jarafael @ inpa. gov. br; https: // orcid. org / 0000 - 0002 - 0170 - 0514 jarafael@inpa.gov.br text Zootaxa 2022 2022-11-16 5209 2 151 186 journal article 188711 10.11646/zootaxa.5209.2.1 40304ff0-808f-4371-a80c-4f0b7c27232f 1175-5326 7325889 710CB5B2-3A7A-491D-A317-CC05419F7A6B Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira, 2013 ( Figs 1–4 , 23 ) Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira, 2013: 185 , figs. 14–24; Lamas & Camargo, 2021 (CTFB catalogue). Diagnosis. Face yellowish brown pruinose; mystical macrosetae yellowish with four dark brown macrosetae dorsally ( Fig. 1C–D ); scape yellow with apical dark ring; pedicel dark brown; postpedicel black, discoidal, laterally compressed; second article of stylus with a distinct, basal, rounded, laterally compressed, lobular projection, ventrally directed (in males) ( Fig. 2A–B ); thorax overall pruinosity yellow to dark brown( Fig.2E ); wings yellowish translucent ( Figs 1B , 2C ); legs dark yellow ( Fig. 1A ); terminalia dark brown; epandrial arms rounded distally, inwardly curved apically ( Figs 3B–C, G–H ); hypandrium rectangular with a short tongue-like mid-posterior projection bifid apically ( Fig. 3D ); gonocoxite tapering until apex strongly inwardly curved apically forming a rounded indentation on internal margin ( Fig. 4B–C ); gonostylus thicker with a slight concavity dorsally, sub-apically ( Fig. 4F ). Redescription. Holotype male ( Figs 1–4 ). Head ( Fig. 1C–D ): eyes black; face reddish brown with yellowish brown pruinosity; mystacal macrosetae and setae yellowish with four dark brown macroseta dorsally; gena black, yellowish pruinose; palpus dark yellow, apex dark brown, with yellow to dark brown setae, except at base ventrally with yellowish white setae, apical setae longer than others; proboscis black; labial setae yellowish and proboscial setae whitish; frons black, yellowish pruinose; orbital setae dark yellow, dark brown dorsally; ocellar tubercle black, sparsely yellowish pruinose with two proclinate black setae between posterior ocelli; vertex yellowish white pruinose, laterally yellowish pruinose with sparse yellowish setae; occiput whitish pruinose; 10–11 yellowish postocular macrosetae; occipital and lower occipital setae white. Antenna ( Fig. 2A–B ): scape yellow with a dark brown ring apically, yellow setose ventrally and black dorsally; pedicel dark brown with black setae; scape and pedicel light yellow pruinose; postpedicel black, discoidal, laterally compressed, broadly truncate apically, slightly shorter than scape in length with sparse golden brown pruinosity and 4–5 extremely short dark brown setae middorsally; stylus black, first article tubular, minute; second article with a basal, rounded, laterally compressed, lobular projection, ventrally directed with almost half the length of postpedicel, remainder of article thin, only slightly enlarged sub-apically and tapering apically, tip dark reddish yellow. Thorax ( Figs 1A–B , 2D–E ): dark reddish to black; paramedian stripes, pre and postsutural spots black pruinose; median stripe dark brown pruinose; scutum anterolaterally and laterally golden yellow pruinose; postalar wall and scutellum sparsely yellowish pruinose ( Fig. 2D ); postpronotal lobe dark reddish and yellowish white pruinose; mesothoracic spiracle whitish pruinose; pleura white pruinose, anterior half of anepisternum, anterior and ventral half of katepisternum and mid-ventral spot on metanepisternum with black tegument. Chaetotaxy: antepronotal macrosetae dark yellow with yellowish setae; 2 notopleural, 1 supra alar, 1 postalar and 2 pairs of postsutural dorsocentral black macrosetae; scutum with short, black acrostichal setae and thin pale yellowish setae laterally before postalar callus; scutellum with short, sparse, yellow discal scutellar setae ( Fig. 2D ); katatergite with yellow macrosetae and setae; anepisternum dorsally with short black setae before wing insertion and a few whitish setae posteroventrally; proepisternum sparsely whitish setose; remainder pleura with scattered whitish setae. Wings ( Figs 1B , 2C ): yellowish translucent; veins dark brown, except veins Sc and R 1 reddish brown and veins at base of wing reddish yellow; bifurcation of R 4 and R 5 beyond discal cell at a distance equivalent to the length of one crossvein r-m ; crossvein r-m in the middle of discal cell; cells m 3 and cua closed before wing margin. Halteres: yellow, base of stem and knob slightly darker. Legs ( Fig. 1A ): dark yellow, except coxae dark reddish to black, femora anterodorsally slightly darker, apex of hind tibia reddish, apex of tarsomeres of fore and mid tarsi and hind tarsus dark reddish, and tarsi with last tarsomeres black on apical half. Chaetotaxy: coxae with yellowish macrosetae and setae; hind trochanter with 3 posteroventral yellow macroseta; fore femur with 6–7 yellow ventral macrosetae; mid femur with 8–10 yellow ventral macrosetae, 4 anteroventral (basal one yellow), 3 anterior, 1 posterodorsal apical black macrosetae and 1 posterior yellow macrosetae; hind femur with 5 anteroventral (basal one yellow), 2 anterodorsal (basal one yellow), 1 pair dorsal preapical, 2 posterodorsal apical, 1 posterior (yellow) and 4 ventral (yellow) black macrosetae; fore femur with short dorsal black setae, other short setae on fore, mid and hind femora yellowish; tibiae with black macrosetae, black setae and with short posterodorsal yellowish setae; tarsi with black macrosetae and setae; brush setae golden yellow; pulvilli dark yellow; empodium reddish yellow; claws black, except basal extremity dark reddish. FIGURE 1. Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira, 2013 , holotype male. A. Habitus, lateral (insert: labels, not to scale); B. Same, dorsal; C. Head, frontal; D. Same, lateral. Scale bar 1 mm. FIGURE 2. Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira, 2013 , holotype male. A. Postpedicel and stylus, anterior; B. Same, dorsal; C. Wing, dorsal; D. Scutellum, dorsal; E. Head and thorax, dorsal; F. Abdomen, lateral; G. Same, dorsal. Abbreviations: f a styl: first article of stylus; ped: pedicel; pped: postpedicel; s a styl: second article of stylus. Scale bar 1 mm. FIGURE 3. Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira, 2013 , holotype male. A. Terminalia, lateral; B. Same, dorsal; C. Same, ventral; D. Hypandrium, ventral; E. Tergite 8, dorsal; F. Sternite 8, ventral; G. Epandrium, dorsal; H. Same, ventral; I. Same, lateral. Abbreviations: cerc: cercus; epand: epandrium; goncx: gonocoxite; gonst: gonostylus; hypd: hypandrium; subepand scl: subepandrial sclerite. Scale bar 1 mm. FIGURE 4. Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira, 2013 , holotype male. A. Terminalia, lateral; B. Gonopods, dorsal; C. Same, ventral; D. Same, lateral; E. Gonocoxite, lateral; F. Gonostylus, lateral; G. Phallus, lateral; H. Cercus and subepandrial sclerite, lateral; I. Same, ventral. Abbreviations: cerc: cercus; ej apod: ejaculatory apodeme; goncx: gonocoxite; goncx apod: gonocoxal apodeme; gonst: gonostylus; ph: phallus; subepand scl: subepandrial sclerite. Scale bar 1 mm. Abdomen ( Fig. 1F–G ): dark brown to almost black; T1 reddish on posterolateral corners; tergites with lateral margins reddish; tergites pruinosity obscured by grease; T1 with 6 yellow lateral marginal macrosetae and yellow setae laterally and at posterior margin; T2–3 with 5–6 lateral marginal yellow macrosetae; T4–8 with 4–5 lateral marginal yellow macrosetae; other short setae on tergites yellowish; sternites with sparse yellowish setae. Terminalia ( Figs 3–4 ): dark brown, except epandrium anterolaterally and laterally black; epandrium black setose, except anterolaterally yellowish setose; cercus dark brown setose; hypandrium yellowish setose with 3– 4 sub-marginal yellowish macrosetae at mid-posterior margin; hypandrium rectangular with a short tongue-like projection at mid-posterior margin, bifid distally ( Fig. 3D ); epandrial arms rounded distally in lateral view ( Figs 3A, 3I ), inwardly curved apically ( Figs 3B–C, 3G–H ); cercus conical; subepandrial sclerite conical with a small sub-triangular projection at base laterally with rounded edges ( Fig. 4H–I ); gonocoxite tapered until apex, strongly inwardly curved apically forming a rounded indentation on internal margin ( Fig. 4B–C ) and without an indentation sub-apically on ventral margin ( Fig. 4D–E ); gonostylus thicker with a slight concavity dorsally, sub-apically, apex truncate and with a very short (almost indistinguishable), comma-like projection mid-ventrally, sparsely covered with short setae on dorsal posterior half ( Fig. 4F ). Length. Body: 14.4 mm ; wing: 9.6 mm . Holotype condition. Right postpedicel ( Figs 1C , 2E ) and right mid leg missing ( Fig. 1A ); abdomen glued between T4 and 5 ( Fig. 2F–G ); right wing detached and mounted on microslide ( Fig. 2C ); terminalia placed in microvial with glycerin and pinned with the specimen. Female. Unknown. However, there is a single female specimen from the locality of Parque Nacional de Sete Cidades, Piauí state ( Fig. 23 (indicated by a question mark)), which could possibly belong to this species. This locality is approximately 400 km away in a straight line northeast of the type locality of C. brachylobus . Through morphological comparison we discarded the possibility of this female belonging to any of the other three species of Cerozodus , being most similar to C. brachylobus . However, only the male holotype specimen was available for study, and the pruinosity of the thorax and abdomen is partially masked by greasing in this specimen. Additionally, this female is slightly smaller in body size and has the colour of legs slightly darker. This kind of intraspecific variation was also observed in other Cerozodus species and in the paratype of C. brachylobus ( Vieira et al . 2013 ) . Consequently, the hypothesis of this female belonging to a new species must be taken into account. Since we could not confidently assign it to C. brachylobus , we decided to suggest that the existence of this female specimen is an incentive to further collections in the same locality to gather more specimens and solve this case. The label data on this specimen is: “ Brasil , Piauí , [Parque Nacional de] Sete Cidades [ 04°05′59″S 41°42′50″W ], Malaise Bananeira, 07–13.ii.2013 ( 1 ♀ INPA ) ”. Variation. Male paratype with postocular setae black; mesonotum golden to brown pruinose; mid and hind femora dark brown; body length 14.6 mm and wing length 9.5 mm . Paratype not examined in this study, information is based on Vieira et al . (2013) . Distribution. Brazil ( Maranhão ) ( Fig. 23 ). Material examined. Holotype . BRASIL , MA[ranhão], Mirador , Parque Est.[adual] Mirador , Base da Geraldina [ 06°37′25″S , 45°52′08″W ] / Armadilha Malaise, 18–23.ix.2006 , F. Limeira-de-Oliveira / Holótipo Cerozodus brachylobus Vieira, Rafael & Limeira-de-Oliveira ( Fig. 1A [insert]) ( ♂ INPA ) . Paratype . Same data, 23–28.ix.2006 {not examined} ( 1 ♂ CZMA ) .