Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations Author Chakraborty, Dyutiparna Author Vizzini, Alfredo Author Das, Kanad text MycoKeys 2018 33 103 124 http://dx.doi.org/10.3897/mycokeys.33.23703 journal article http://dx.doi.org/10.3897/mycokeys.33.23703 1314-4049--103 Tylopilus neofelleus Hongo, J. Jpn. Bot. 42: 154 (1967) Figs 7, 8 Description. Pileus 70-120 mm broad, convex to broadly convex; surface distinctly tomentose to subvelvety, dry, subviscid when wet; reddish-brown (8-9F4) when young, rosewood (9C5) to vinaceous-brown (16C5) with maturity, fawn (7E4) towards margin. Pores yellowish-white or cream with a pinkish tinge, orange-grey (6B2) with age; pore 2-3/mm, rounded. Tubes 10-15 mm, adnate-sinuate, white in colour, yellow-brown or orange white with maturity. Stipe 60-100 x 18-22 mm, cylindrical, solid, surface dry, glabrous to subvelutinous, typically distinctly reticulate at apex, reticulation greyish-ruby (12 C-D 4) to dark ruby (12F5); surface pinkish brown to vinaceous or violaceous, dark brown to reddish-brown with maturity. Context chalky white, but pinkish-brown when exposed. Spore print not obtained. Basidiospores 10 -11.5- 13.5 x 4 -4.6- 5.2 µm (n = 30, Q = 2.05 -2.48- 2.76), ellipsoid to narrowly subfusoid, inequilateral, smooth, thin-walled. Basidia 30-36 x 10-11 µm , 4-spored, clavate, thin-walled, hyaline or pale grey in KOH. Pleurocystidia 35 -66 x 14-24 µm , scattering and numerous, fusoid-ventricose or subclavate, with orange brown contents. Cheilocystidia 33-38 x 9-12 µm , ventricose to fusoid, shorter and smaller than pleurocystidia thin-walled, with orange brown contents. Pileipellis 100-150 µm thick, an ixotrichoderm of suberect, branched, septate hyphae; terminal elements ventricose to fusoid, vaculolated, 28-50 x 12-14 µm , with granular yellowish to brown orange contents in KOH; subterminal elements mostly with incrustations. Stipitipellis 35-65 µm , fertile, composed of basidia and cystidia. Caulocystidia 52-63 x 8-13 µm , fusoid to subfusoid, ventricose to ventricose-rostrate or narrowly cylindrical, content granular. Clamp connection absent in all tissues. Figure 7. Tylopilus neofelleus (DC 16-63). a, b Fresh basidiomata in the field and in basecamp c Stipe surface with reticulation d Pileipellis e Hymenial layer showing basidia and pleurocystidia f Tube edge g Basidiospores. Scale bars: 50 μm (d, f); 10 μm (e, g). Figure 8. Tylopilus neofelleus (DC 16-63). a Basidiospores b Pleurocystidia c Caulocystidia d Pileipellis. Scale bars: 10 μm ( a-d ). Habitat. Under Castanopsis sp. in temperate broadleaf forest. Known distribution. Japan ( Kawamura 1954 ; Hongo 1960 ; Imazeki et al. 1970 , 1988 ; Takahashi 1986 ; Gelardi et al. 2015 ), China ( Ying and Zang 1994 ; Li and Song 2000 ; Wang et al. 2004 ; Fu et al. 2006 ; Wu et al. 2011 ; Gelardi et al. 2015 ), Russia ( Vasil'jeva 1978 ) and New Guinea ( Hongo 1973 ). Specimens examined. India. Sikkim: East district, Fambonglo WLS, 2021 m alt., N27°21'47.5" E88°34'13.2" , 26 Aug 2016, D. Chakraborty & K. Das, DC 16-63 (CAL); ibid., D. Chakraborty & K. Das, DC 16-64 (CAL).