Multigene phylogeny and morphology reveal a new species, Ophiocordyceps tettigonia, from Guizhou Province, China Author Wen, Ting-Chi Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, Guizhou Province, China Author Xiao, Yuan-Pin Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, Guizhou Province, China & Center of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Zha, Ling-Sheng Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, Guizhou Province, China & Center of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Hyde, Kevin D. Center of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Kang, Ji-Chuan Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, Guizhou Province, China text Phytotaxa 2016 2016-10-19 280 2 141 151 http://dx.doi.org/10.11646/phytotaxa.280.2.4 journal article 10.11646/phytotaxa.280.2.4 1179-3163 13645883 Ophiocordyceps tettigonia T.C. Wen, Y.P. Xiao & K. D. Hyde , sp. nov. ( Fig. 2 ) Index Fungorum number : IF551451; Facesoffungi number : FoF01020. Differs from related Ophiocordyceps species mainly by its unique host, and wide secondary ascospores. Type:— CHINA . Guizhou Province : Chishui City, Shizhangdong scenic spot, Chishui Danxia natural world heritage site, on adult of Tettigonia in the soil, 9 June 2014 , Ting-Chi Wen CS14062709 (GZUHCS14062709, holotype ). FIGURE 2. Ophiocordyceps tettigonia (holotype). a. Overview of stromata and the host. b. Pale, superficial ascomata on stroma. c. Cross section showing the complete stroma and perithecia. d–f. Sections of ascomata. g, h. Part of peridium. i–k. Asci. l. Ascus with apical cap. m. Ascus with apical cap stained by iodine solution. n. Ascus stained by iodine solution. o. Secondary ascospores. p. Secondary ascospores stained by iodine solution. Scale bars: d = 1000 μm, e, f = 200 μm, g, h = 30 μm, i–k = 100 μm, l, m, o, p = 10 μm, n = 20 μm. Parasitic on an adult of Tettigonia . Host covered with white, dense mycelium, substratum buried in the soil, red to red-brown. Sexual morph: Stromata 35–40 mm long, 2–2.5 mm wide, growing from the head, with two branches. Stipe 32.5–37.5 mm long, 2 mm wide, flexuous, white. Fertile head 2–2.5 mm wide, globose, differentiated from stipe. Ascomata 520–680 × 205–275 μm ( x = 599 × 242, n = 30), completely immersed, elongated or ampuliform, with the ostioles opening on the surface of the head. Peridium 22.8–35.0 μm ( x = 28.9, n = 30) wide, comprised of three layers. Asci 530–615 × 6.5–9.3 μm ( x = 573.3 × 7.9, n = 30), 8-spored, hyaline, cylindrical, possessing a prominent apical cap. Apical cap 3.9–4.8 μm wide ( x = 4.35, n = 30). Ascospores 350–465 × 1.5–2.2 μm ( x = 407 × 1.8, n = 60), fasciculate, thread-like, slender and long, breaking into secondary ascospores when mature. Secondary ascospores 6.7–9.4 × 1.5–2.3 μm ( x = 8.1 × 1.9, n = 60), hyaline, cylindrical. Asexual morph: Undetermined. Etymology:— Refers to the unique host insect, Tettigonia . Distribution:— Chishui City, Guizhou Province , China . Host:— On adult of Tettigonia living in the soil.