Thirteen new species of Agromyzidae (Diptera) from the United States, with new host and distribution records for 32 additional species Author Eiseman, Charles S. Author Lonsdale, Owen 0000-0001-7034-3330 onsdale@agr.gc.ca Author Linden, John Van Der 0000-0003-2651-0634 johnbvdl@gmail.com Author Feldman, Tracy S. 0000-0001-5939-6810 feldmants@sa.edu Author Palmer, Michael W. 0000-0003-1452-2152 mike.palmer@okstate.edu text Zootaxa 2021 2021-02-17 4931 1 1 68 journal article 7424 10.11646/zootaxa.4931.1.1 d63cab47-63f1-4fdc-9209-26a256c91ccb 1175-5326 4545337 88CF2B0D-E02B-46E1-9F52-1B95F717FC8F Phytomyza krygeri Hering Material examined. MASSACHUSETTS : Franklin Co. , Northfield , 42.647283 , -72.424726 , 20.vi.2017 , em. 26.iv–6.v.2018 , C.S. Eiseman , ex Aquilegia canadensis seedpods, # CSE4441 , CNC1135721–1135728 (5♁ 3♀ ) ; WISCONSIN : Vernon Co. , Battle Bluff Prairie State Natural Area , 43°18′5.04″N 91°48′6.60″W , 13.vi.2017 , em. spring 2018, J. van der Linden , ex Aquilegia canadensis , # CSE4947 , CNC1288661–1288662 ( 2♀ ) . Hosts. Ranunculaceae : Aquilegia * canadensis L. , A. vulgaris L. ( Lonsdale 2017b ). Larval biology. Larvae feed in developing seed pods, which become distorted and discolored brown. Puparium. Formed within the seed pod; subshining and dark brown, almost black; similar in size and appearance to the remains of the slightly paler brown seeds damaged by larval feeding ( Lonsdale 2017b ). Phenology and voltinism. This species is univoltine, overwintering as pupae. In Ottawa, adults are present in May and puparia are formed by the end of June ( Lonsdale 2017b ). Distribution. * USA : [IA], MA, WI; Canada : ON; Europe ( Lonsdale 2017b ). Comments. This European species was discovered ovipositing in flowers of cultivated European columbine in Ottawa, Ontario in the spring of 2015 ( Lonsdale 2017b ). The new rearing records, along with larvae and puparia we observed in northeastern Iowa in June 2018 , indicate that it is already widespread in the USA and utilizing native columbines .