Ontogenetic Instars Of Phyllhermannia Bimaculata Hammer, 1979 (Acari, Oribatida, Hermanniidae) Author Ermilov, Sergey G. Author Salavatulin, Vladimir M. text Acta Zoologica Academiae Scientiarum Hungaricae 2023 2023-02-28 69 1 11 24 http://dx.doi.org/10.17109/azh.69.1.11.2023 journal article 10.17109/AZH.69.1.11.2023 2064-2474 7710008 Phyllhermannia bimaculata Hammer, 1979 ( Figs 1–8 ) Main diagnostic traits of adult – ( Figs 1A–D ; 2A–C ; based on our data and data from HAMMER (1979) and LUXTON (1991)) . Body length: 540–700. Body color brown, covered by thick layer of gel-like cerotegument, partially forming tubercles on dorsal side and foveate-reticulate ornamentation on epimeres I–III and leg segments; cuticle densely porose; epimeral border IV with some tubercles. Rostrum rounded. Dorsal part of prodorsum with two longitudinal, arch-like (concave in median part) ridges; interbothridial part of prodorsum with two oval, convex structures having one posterior tubercle; lateral longitudinal ridge present, extending basally into lateral (anterior) tubercle (opposite posterior lateral tubercle absent). Rostral and lamellar setae short, setiform, barbed; interlamellar seta medium-sized, broadly phylliform, barbed; bothridial seta long, bacilliform proximally and gradually dilating in distal half to well visible, flattened head having microscales; dorsal exobothridial seta spiniform; bs> in> ro = le> exd . Anterodorsal part of notogaster with median longitudinal and one pair of lateral semicircular band-like furrows resembling the shape of two large cells. Notogastral setae medium-sized, broadly phylliform, barbed. Subcapitular setae a and h setiform, roughened, m minute, simple ( m 2 absent); a> h> m ; adoral seta or 1 dilated and truncate distally, or 2 bacilliform. Palp with setation 0–1–1–3–9(+ω). Chelicera with two setiform, barbed setae; cha> chb . Epimeral setal formula: 3–1–3(4)–5(6); lateral setae of epimere III medium-sized, other setae short, setiform, all barbed. Genital, aggenital, adanal, and anal setae setiform, roughened or barbed. Adanal lyrifissure diagonal. Formulas of leg setation and solenidia (see Table 1 ): I (1–6–5–5–20) [1–2–1], II (1–6–5–5–15) [1–1–1], III (2–3–4–4–14) [1–1–0], IV (1–3–4–4–14) [0–1–0]; tc’ elongate phylliform on tarsi III, IV. Description of juvenile instars ( Figs 2D ; 3A, B ; 4A–E ; 5A, B ; 6A, B ; 7A–D ; 8A–D ) – Measurements. Total length of larva: 270–285 (n = 16), protonymph: 330–345 (n = 8), deutonymph: 345–375 (n = 9), tritonymph: 435–510 (n = 4). Total width of larva: 120–135 (n = 16), protonymph: 150–173 (n = 8), deutonymph: 165–180 (n = 9), tritonymph: 225–240 (n = 4). Integument . Body cuticle colorless (larva and protonymph) to light brownish (deuto- and tritonymph). Cuticle porose. Prodorsum with rare ridges; two of them well developed, diagonal, convergent, connected or separated medially. Interlamellar (between bothridium and prodorsal median ridge) and posterolateral gastronotic regions, and lateral parts of epimeres slightly tuberculate-reticulate in nymphal instars (versus indistinct in larva). Gastronotic and anogenital regions folded. Genital valves longitudinally striate. Subcapitular mentum and leg segments partially foveate-reticulate in nymphal instars. Prodorsum . Triangular, about 2/3 length of gastronotic region. Rostrum rounded. Rostral seta (La: 5; Pn, Dn: 9–11; Tn: 15) setiform, erect, slightly barbed, divergent, located on tubercle. Lamellar seta (La: 5; Pn, Dn: 7–9; Tn: 9–11) similar in morphology to rostral setae in larva and protonymph versus slightly thickened or indistinctly phylliform in deuto-and tritonymph. Interlamellar seta (La: 5–7; Pn: 9–11; Dn: 15–22; Tn: 19–26) broadly phylliform, barbed. Bothridial seta (La: 60–64; Pn: 71–75; Dn: 82–86; Tn: 90–94) bacilliform proximally and gradually dilating in distal half to well visible, flattened head having microscales. Dorsal exobothridial seta (La, Pn, Dn: 7; Tn: 7–9) spiniform, smooth, located very close to bothridium. Ventral exobothridial seta represented by alveolus. Fig. 1. Phyllhermannia bimaculata Hammer, 1979 , adult, SEM micrographs: A, B = dorsal view; C = ventral view; D = ventrolateral view Gastronotic region . Prodorsum and gastronotum separated by folds. Posterior part rounded. Longitudinal ecdysial cleavage line well visible (transverse line not observed). Larva with 13 pairs of setae; h 3 vestigial, h 2 (13–15) setiform, erect, roughened, other setae (7) broadly phylliform, barbed. Nymphal instars with 16 pairs (Pn: p 1 , p 2 : 7–9, others: 9–11; Dn: p 1 , p 2 : 9–11, others: 15–22; Tn: p 1 , p 2 : 11, others: 19–26) of broadly phylliform, barbed setae (except p 2 and p 3 setiform, roughened in protonymph). Cupules ia and im and opisthonotal gland opening (near seta f 2 ) visible, cupule ip not observed. Gnathosoma . Subcapitulum size: La: 52–56 × 52–56; Pn: 71 × 64; Dn: 90 × 79; Tn: 97 × 86. Subcapitular setae a and h (La: a : 15–19, h : 9–11; Pn: a : 19, h : 11; Dn, Tn: a : 22–26, h : 15) setiform, roughened; m vestigial. Two pairs of adoral setae present; adoral seta or 1 (La: 7–9; Pn: 9; Dn, Tn: 11) dilated and truncate distally, or 2 (La: 7–9; Pn: 9; Dn, Tn: 11) setiform. Palp notogastral cerotegument; D = dorsal view Fig. 2. Phyllhermannia bimaculata Hammer, 1979 , adult (A–C) and protonymph (D), SEM micrographs: A = dorsoanterior view; B = right lateral view; C = notogastral seta e 1 and (La: 30; Pn: 37; Dn: 45; Tn: 52) setation: 0–1–1–3–9(+ω). Postpalpal seta (length: La: 15–19; Pn, Dn, Tn: 15) setiform, blunt-ended, erect, roughened. Chelicera (length: La: 64; Pn: 79; Dn: 101; Tn: 112) with two setiform, barbed setae ( cha : La: 15–19; Pn: 22; Dn: 30; Tn: 34) distinctly barbed; chb : La: 9–11; Pn: 13; Dn: 17–19; Tn: 19–22) barbed. Epimeral and lateral podosomal regions . Setal formulas for epimeres: La: 3–1–2 (third seta of first epimere forms protective scale over respective Claparède’s organ); Pn: 3–1–2–1; Dn: 3–1–2(sometimes 3)–3; Tn: 3–1–3–4. Epimeral setae (La, Pn: 7; Dn, Tn: 9–11) setiform, erect, roughened but setae 1a , 2a and 3a distally bifurcate in larval instar. Anogenital region . Ontogeny of genital, aggenital, adanal, and anal setal formulas, larva to tritonymph, 0–1–4–7 (Pn: 11; Dn: one pair: 19–26, others: 7–11; Tn: one pair: 26–33, others: 9–11), 0–0–1–2 (Dn, Tn: 9–11), 0–0–3–3 (Dn, Tn: 9–11), 0–0–0–2 (Tn: 9–11), respectively. All setae setiform, roughened to barbed. Cupules ih , ips , iad appearing in normal ontogenetic pattern; becoming inconspicuous when shifted away from anal valves. Legs . Claw of each leg strong, slightly barbed on dorsal side. Femora I–IV and trochanters III, IV with poorly visible ventroparaxial porose area. Formulas of leg setation and solenidia (see Table 1 for designations): La: I (0–2–3–4–16) [1–1–1], II (0–2–3–4–13) [1–1–1], III (0–2–2–3–12) [1–1–0]; Pn: I (0–4–4–5–16) [1–1–1], II (0–4–4–4–13) [1–1–1], III (1–2–2–3– 12) [1–1–0], IV (0–0–1–0–7) [0–0–0]; Dn: I (1–5–4–5–16) [1–2–1], II (1–5–4–5–13) [1–1–1], III (2–3–2–3–12) [1–1–0], IV (1–2–2–3–12) [0–1–0]; Tn: I (1–5–5–5–18) [1–2–1], II (1–5–5–5–13) [1–1–1], III (2–3–3–4–12) [1–1–0], IV (1–3–3–4–12) [0–1–0]. Tibiae I–IV and genua I–III with seta d coupled with respective solenidion; it” , it’ absent on all tarsi; tc’ elongate phylliform on tarsi III, IV; ft’ absent on tarsus III.